Description
Categories of SUIT protocols group SUIT protocols according to all ETS substrate types involved in a protocol, independent of titrations of inhibitors which block the oxidation of the substrates present. ROX states may or may not be included in a SUIT protocol, which does not change its category. Β» MiPNet article
Abbreviation: SUIT-Catg
Reference: MiPNet21.06 SUIT-RP
MitoPedia concepts:
MiP concept,
SUIT concept
Categorization of SUIT protocols: ETS substrate types
Gnaiger E (2016) Categorization of SUIT protocols: ETS substrate types. MiPNet 2016-03-20. |
Abstract: There are many ways to define groups of SUIT protocols. The complexity of SUIT protocols is primarily determined by the large number of possible substrate control states, compared to only three well defined coupling control states. Therefore, a relevant type of categories of SUIT protocols considers the ETS substrate types involved. Whereas the SUIT protocol names include all specific substrates applied in the SUIT protocol, the categories of SUIT protocols reduce this diversity to ETS substrate types
β’ O2k-Network Lab: AT Innsbruck OROBOROS
Labels: MiParea: Instruments;methods
HRR: Theory
Towards a library of SUIT protocols
- At the present stage of development of the 'library of SUIT protocols' as part of the MitoFit Quality Control System, five ETS substrate types are considered.
- N and F on the pathway level of converging NADH- and FADH2-linked dehydrogenases, including the TCA cycle and beta-oxidation.
- S and Gp on the pathway level of electron transfer complexes converging at the Q-junction.
- Tm on the single step level of cytochrome c oxidase (CIV), the terminal step in the aerobic electron transfer system. Tm can be included or excluded at the end of a SUIT protocol. To simplify the categorization, Tm is not considered in this system of SUIT protocols.
- At the present stage of development of the 'library of SUIT protocols' as part of the MitoFit Quality Control System, five ETS substrate types are considered.
SUIT-Catg: single substrate type
- N - ETS-level 4: NADH-linked substrates (CI-linked)
- F - ETS-level 4: FADH2-linked substrates (FAO)
- S - ETS-level 3: Succinate (CII-linked)
- Gp - ETS-level 3: Glycerophosphate (CGpDH-linked)
SUIT-Catg: multiple ETS substrate types with NS
NS
NFS
NSGp
NFSGp
SUIT-Catg: multiple ETS substrate types with N (without S)
NF
NGp
NFGp
SUIT-Catg: multiple ETS substrate types without N
- F and S without N are problematic substrate states due to accumulation of Oxa or Acetyl-CoA. Therefore, addition of malate alone (M without P or G) is not considered as substrate type N. However, high mt-malic enzyme activity requires a change of this concept, when M alone represents an ETS ccompetent substrate state. Low concentration of M may be used to support FAO, whereas a higher concentration of M may be required for N-linked respiratory capacity to override FAO capacity; this needs corresponding kinetic analyses (SUIT test protocols).