Ascorbate: Difference between revisions
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{{MitoPedia | {{MitoPedia | ||
|abbr=As | |abbr=As | ||
|description=In respiratory assays for cytochrome c oxidase (COX; | |description=In respiratory assays for cytochrome c oxidase (COX; complex CIV) activity, '''ascorbate''' is added as regenerating system for reduced [[TMPD|TMPD]]. | ||
|info=MiPNet06.06 | |info=MiPNet06.06 | ||
}} | }} |
Revision as of 09:02, 16 May 2012
Description
In respiratory assays for cytochrome c oxidase (COX; complex CIV) activity, ascorbate is added as regenerating system for reduced TMPD.
Abbreviation: As
Reference: MiPNet06.06
MitoPedia topics:
Substrate and metabolite
Application in HRR
As: Ascorbate sodium salt (Succinate disodium salt, hexahydrate, C6H706Na); Sigma, A 4034, 100 g, store at R.T.): FW = 198.1
Caution: Light sensitive (store solution in a dark glass vial).
Preparation of 0.8 M stock solution (dissolved in H2O):
- 1) weigh 1.584 g of Ascorbate sodium salt and dissolve in 10 ml H2O
- 2) To prevent autooxidation, adjust pH to ca 6 with ascorbic acid (a 137.6 mg ml-1 solution of pH ca 2).
- 3) Divide into 0.2 ml portions.
- 4) Store frozen at -20 °C.
Oxygraph-2k Manual Titrations: MiPNet09.12