Azide: Difference between revisions

Revision as of 10:12, 11 September 2020

high-resolution terminology - matching measurements at high-resolution

Azide

Description

Sodium azide is an inhbitor of cytochrome c oxidase (COX, CcO).

Abbreviation: Azd

Reference: MiPNet03.02

Application in HRR

Azd: Azide (Sodium azide, NaN₃); Sigma S2002, SDS 25 g, store commercial stock at RT; FW = 65.01.

Caution: Very toxic!

Preparation of 4 M storage and stock solution (dissolved in H₂O):

Weigh 260 mg of sodium azide.
Dissolve in 1 mL H₂0.
Divide into 0.5 mL portions.
Store at -20 °C.

»O2k manual titrations MiPNet09.12 O2k-Titrations

Titration volume: ≥50 µL using a 100 µL syringe (2 mL O2k-Chamber).
Final concentration: ≥100 mM.

MitoPedia topics: Inhibitor

@@ Line 9: / Line 9: @@
 == Application in [[HRR]] ==
-'''Azd: Azide''' (Sodium azide, NaN<sub>3</sub>); [http://www.sigmaaldrich.com/catalog/product/sial/s2002?lang=en&region=GB Sigma S2002], [http://www.sigmaaldrich.com/MSDS/MSDS/DisplayMSDSPage.do?country=GB&language=en&productNumber=S2002&brand=SIAL&PageToGoToURL=http%3A%2F%2Fwww.sigmaaldrich.com%2Fcatalog%2Fproduct%2Fsial%2Fs2002%3Flang%3Den SDS] 25 g, store commercial stock at RT; FW = 65.01.
+:::'''Azd: Azide''' (Sodium azide, NaN<sub>3</sub>); [http://www.sigmaaldrich.com/catalog/product/sial/s2002?lang=en&region=GB Sigma S2002], [http://www.sigmaaldrich.com/MSDS/MSDS/DisplayMSDSPage.do?country=GB&language=en&productNumber=S2002&brand=SIAL&PageToGoToURL=http%3A%2F%2Fwww.sigmaaldrich.com%2Fcatalog%2Fproduct%2Fsial%2Fs2002%3Flang%3Den SDS] 25 g, store commercial stock at RT; FW = 65.01.
-<span style="color:#8B008B"> '''Caution:''' Very toxic!</span>
+::::<span style="color:#8B008B"> '''Caution:''' Very toxic!</span>
-'''Preparation of 4 M storage and stock solution''' (dissolved in H<sub>2</sub>O):
+::::'''Preparation of 4 M storage and stock solution''' (dissolved in H<sub>2</sub>O):
-::1) Weigh 260 mg of sodium azide.
+::::# Weigh 260 mg of sodium azide.
-::2) Dissolve in 1 mL H<sub>2</sub>0.
+::::# Dissolve in 1 mL H<sub>2</sub>0.
-::3) Divide into 0.5 mL portions.
+::::# Divide into 0.5 mL portions.
-::4) Store at -20 °C.
+::::# Store at -20 °C.
-'''O2k manual titrations'''  [[MiPNet09.12 O2k-Titrations]]
+:::»'''O2k manual titrations'''  [[MiPNet09.12 O2k-Titrations]]
-::* Titration volume: ≥50 µL using a 100 µL syringe (2 mL O2k-Chamber).
+::::* Titration volume: ≥50 µL using a 100 µL syringe (2 mL O2k-Chamber).
-::* Final concentration: ≥100 mM.
+::::* Final concentration: ≥100 mM.