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Belosludtseva 2022 Membranes (Basel) - Revision history
2024-03-28T22:58:22Z
Revision history for this page on the wiki
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Plangger Mario at 14:35, 8 August 2022
2022-08-08T14:35:58Z
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<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 14:35, 8 August 2022</td>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|area=Respiration</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|area=Respiration</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>|instruments=Oxygraph-2k</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">|organism=Rat</ins></div></td></tr>
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<tr><td colspan="2"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">|preparations=Isolated mitochondria</ins></div></td></tr>
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Plangger Mario
https://wiki.oroboros.at/index.php?title=Belosludtseva_2022_Membranes_(Basel)&diff=231048&oldid=prev
Plangger Mario at 14:06, 8 August 2022
2022-08-08T14:06:37Z
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<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 14:06, 8 August 2022</td>
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<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>|title=Belosludtseva NV, Pavlik LL, Belosludtsev KN, Saris NL, Shigaeva MI, Mironova GD (2022) The short-term opening of cyclosporin A-independent palmitate/Sr <sup>2+</sup>-induced pore can underlie ion efflux in the oscillatory mode of functioning of rat liver mitochondria.</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>|title=Belosludtseva NV, Pavlik LL, Belosludtsev KN, Saris NL, Shigaeva MI, Mironova GD (2022) The short-term opening of cyclosporin A-independent palmitate/Sr <sup>2+</sup>-induced pore can underlie ion efflux in the oscillatory mode of functioning of rat liver mitochondria. <ins style="font-weight: bold; text-decoration: none;">https://doi.org/10.3390/membranes12070667</ins></div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|info=Membranes (Basel) 12:667. [https://www.ncbi.nlm.nih.gov/pubmed/35877870 PMID: 35877870 Open Access]</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|info=Membranes (Basel) 12:667. [https://www.ncbi.nlm.nih.gov/pubmed/35877870 PMID: 35877870 Open Access]</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|authors=Belosludtseva Natalia V, Pavlik Lyubov L, Belosludtsev Konstantin N, Saris Nils-Erik L, Shigaeva Maria I, Mironova Galina D</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|authors=Belosludtseva Natalia V, Pavlik Lyubov L, Belosludtsev Konstantin N, Saris Nils-Erik L, Shigaeva Maria I, Mironova Galina D</div></td></tr>
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Plangger Mario
https://wiki.oroboros.at/index.php?title=Belosludtseva_2022_Membranes_(Basel)&diff=231047&oldid=prev
Plangger Mario at 14:05, 8 August 2022
2022-08-08T14:05:10Z
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<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 14:05, 8 August 2022</td>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|year=2022</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|year=2022</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|journal=Membranes (Basel)</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|journal=Membranes (Basel)</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>|abstract=Mitochondria are capable of synchronized oscillations in many variables, but the underlying mechanisms are still unclear. In this study, we demonstrated that rat liver mitochondria, when exposed to a pulse of <del style="font-weight: bold; text-decoration: none;">Sr2</del>+ ions in the presence of valinomycin (a potassium ionophore) and cyclosporin A (a specific inhibitor of the permeability transition pore complex) under hypotonia, showed prolonged oscillations in K+ and <del style="font-weight: bold; text-decoration: none;">Sr2</del>+ fluxes, membrane potential, pH, matrix volume, rates of oxygen consumption and <del style="font-weight: bold; text-decoration: none;">H2O2 </del>formation. The dynamic changes in the rate of <del style="font-weight: bold; text-decoration: none;">H2O2 </del>production were in a reciprocal relationship with the respiration rate and in a direct relationship with the mitochondrial membrane potential and other indicators studied. The pre-incubation of mitochondria with <del style="font-weight: bold; text-decoration: none;">Ca2</del>+(<del style="font-weight: bold; text-decoration: none;">Sr2</del>+)-dependent phospholipase <del style="font-weight: bold; text-decoration: none;">A2 </del>inhibitors considerably suppressed the accumulation of free fatty acids, including palmitic and stearic acids, and all spontaneous <del style="font-weight: bold; text-decoration: none;">Sr2</del>+-induced cyclic changes. These data suggest that the mechanism of ion efflux from mitochondria is related to the opening of short-living pores, which can be caused by the formation of complexes between <del style="font-weight: bold; text-decoration: none;">Sr2</del>+(<del style="font-weight: bold; text-decoration: none;">Ca2</del>+) and endogenous long-chain saturated fatty acids (mainly, palmitic acid) that accumulate due to the activation of phospholipase <del style="font-weight: bold; text-decoration: none;">A2 </del>by the ions. A possible role for transient palmitate/<del style="font-weight: bold; text-decoration: none;">Ca2</del>+(<del style="font-weight: bold; text-decoration: none;">Sr2</del>+)-induced pores in the maintenance of ion homeostasis and the prevention of calcium overload in mitochondria under pathophysiological conditions is discussed.</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>|abstract=Mitochondria are capable of synchronized oscillations in many variables, but the underlying mechanisms are still unclear. In this study, we demonstrated that rat liver mitochondria, when exposed to a pulse of <ins style="font-weight: bold; text-decoration: none;">Sr<sup>2</ins>+<ins style="font-weight: bold; text-decoration: none;"></sup> </ins>ions in the presence of valinomycin (a potassium ionophore) and cyclosporin A (a specific inhibitor of the permeability transition pore complex) under hypotonia, showed prolonged oscillations in K<ins style="font-weight: bold; text-decoration: none;"><sup></ins>+<ins style="font-weight: bold; text-decoration: none;"></sup> </ins>and <ins style="font-weight: bold; text-decoration: none;">Sr<sup>2</ins>+<ins style="font-weight: bold; text-decoration: none;"></sup> </ins>fluxes, membrane potential, pH, matrix volume, rates of oxygen consumption and <ins style="font-weight: bold; text-decoration: none;">H<sub>2</sub>O<sub>2</sub> </ins>formation. The dynamic changes in the rate of <ins style="font-weight: bold; text-decoration: none;">H<sub>2</sub>O<sub>2</sub> </ins>production were in a reciprocal relationship with the respiration rate and in a direct relationship with the mitochondrial membrane potential and other indicators studied. The pre-incubation of mitochondria with <ins style="font-weight: bold; text-decoration: none;">Ca<sup>2</ins>+<ins style="font-weight: bold; text-decoration: none;"></sup></ins>(<ins style="font-weight: bold; text-decoration: none;">Sr<sup>2</ins>+<ins style="font-weight: bold; text-decoration: none;"></sup></ins>)-dependent phospholipase <ins style="font-weight: bold; text-decoration: none;">A<sub>2</sub> </ins>inhibitors considerably suppressed the accumulation of free fatty acids, including palmitic and stearic acids, and all spontaneous <ins style="font-weight: bold; text-decoration: none;">Sr<sup>2</ins>+<ins style="font-weight: bold; text-decoration: none;"></sup></ins>-induced cyclic changes. These data suggest that the mechanism of ion efflux from mitochondria is related to the opening of short-living pores, which can be caused by the formation of complexes between <ins style="font-weight: bold; text-decoration: none;">Sr<sup>2</ins>+<ins style="font-weight: bold; text-decoration: none;"></sup></ins>(<ins style="font-weight: bold; text-decoration: none;">Ca<sup>2</ins>+<ins style="font-weight: bold; text-decoration: none;"></sup></ins>) and endogenous long-chain saturated fatty acids (mainly, palmitic acid) that accumulate due to the activation of phospholipase <ins style="font-weight: bold; text-decoration: none;">A<sub>2</sub> </ins>by the ions. A possible role for transient palmitate/<ins style="font-weight: bold; text-decoration: none;">Ca<sup>2</ins>+<ins style="font-weight: bold; text-decoration: none;"></sup></ins>(<ins style="font-weight: bold; text-decoration: none;">Sr<sup>2</ins>+<ins style="font-weight: bold; text-decoration: none;"></sup></ins>)-induced pores in the maintenance of ion homeostasis and the prevention of calcium overload in mitochondria under pathophysiological conditions is discussed.</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|keywords=Cyclosporin A, Cyclosporin A-independent palmitate/Ca2+-induced permeability transition pore, Ion oscillations, Lipid pore, Mitochondria, Mitochondrial permeability transition, Palmitic acid, Phospholipase A2</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|keywords=Cyclosporin A, Cyclosporin A-independent palmitate/Ca2+-induced permeability transition pore, Ion oscillations, Lipid pore, Mitochondria, Mitochondrial permeability transition, Palmitic acid, Phospholipase A2</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|editor=[[Plangger M]]</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|editor=[[Plangger M]]</div></td></tr>
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Plangger Mario
https://wiki.oroboros.at/index.php?title=Belosludtseva_2022_Membranes_(Basel)&diff=231046&oldid=prev
Plangger Mario at 14:01, 8 August 2022
2022-08-08T14:01:49Z
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<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 14:01, 8 August 2022</td>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|title=Belosludtseva NV, Pavlik LL, Belosludtsev KN, Saris NL, Shigaeva MI, Mironova GD (2022) The short-term opening of cyclosporin A-independent palmitate/Sr <sup>2+</sup>-induced pore can underlie ion efflux in the oscillatory mode of functioning of rat liver mitochondria.</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|title=Belosludtseva NV, Pavlik LL, Belosludtsev KN, Saris NL, Shigaeva MI, Mironova GD (2022) The short-term opening of cyclosporin A-independent palmitate/Sr <sup>2+</sup>-induced pore can underlie ion efflux in the oscillatory mode of functioning of rat liver mitochondria.</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|info=Membranes (Basel) 12:667. [https://www.ncbi.nlm.nih.gov/pubmed/35877870 PMID: 35877870 Open Access]</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|info=Membranes (Basel) 12:667. [https://www.ncbi.nlm.nih.gov/pubmed/35877870 PMID: 35877870 Open Access]</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>|authors=Belosludtseva <del style="font-weight: bold; text-decoration: none;">NV</del>, Pavlik <del style="font-weight: bold; text-decoration: none;">LL</del>, Belosludtsev <del style="font-weight: bold; text-decoration: none;">KN</del>, Saris <del style="font-weight: bold; text-decoration: none;">NL</del>, Shigaeva <del style="font-weight: bold; text-decoration: none;">MI</del>, Mironova <del style="font-weight: bold; text-decoration: none;">GD</del></div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>|authors=Belosludtseva <ins style="font-weight: bold; text-decoration: none;">Natalia V</ins>, Pavlik <ins style="font-weight: bold; text-decoration: none;">Lyubov L</ins>, Belosludtsev <ins style="font-weight: bold; text-decoration: none;">Konstantin N</ins>, Saris <ins style="font-weight: bold; text-decoration: none;">Nils-Erik L</ins>, Shigaeva <ins style="font-weight: bold; text-decoration: none;">Maria I</ins>, Mironova <ins style="font-weight: bold; text-decoration: none;">Galina D</ins></div></td></tr>
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Plangger Mario
https://wiki.oroboros.at/index.php?title=Belosludtseva_2022_Membranes_(Basel)&diff=231045&oldid=prev
Plangger Mario at 14:00, 8 August 2022
2022-08-08T14:00:25Z
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<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">← Older revision</td>
<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 14:00, 8 August 2022</td>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|journal=Membranes (Basel)</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|journal=Membranes (Basel)</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|abstract=Mitochondria are capable of synchronized oscillations in many variables, but the underlying mechanisms are still unclear. In this study, we demonstrated that rat liver mitochondria, when exposed to a pulse of Sr2+ ions in the presence of valinomycin (a potassium ionophore) and cyclosporin A (a specific inhibitor of the permeability transition pore complex) under hypotonia, showed prolonged oscillations in K+ and Sr2+ fluxes, membrane potential, pH, matrix volume, rates of oxygen consumption and H2O2 formation. The dynamic changes in the rate of H2O2 production were in a reciprocal relationship with the respiration rate and in a direct relationship with the mitochondrial membrane potential and other indicators studied. The pre-incubation of mitochondria with Ca2+(Sr2+)-dependent phospholipase A2 inhibitors considerably suppressed the accumulation of free fatty acids, including palmitic and stearic acids, and all spontaneous Sr2+-induced cyclic changes. These data suggest that the mechanism of ion efflux from mitochondria is related to the opening of short-living pores, which can be caused by the formation of complexes between Sr2+(Ca2+) and endogenous long-chain saturated fatty acids (mainly, palmitic acid) that accumulate due to the activation of phospholipase A2 by the ions. A possible role for transient palmitate/Ca2+(Sr2+)-induced pores in the maintenance of ion homeostasis and the prevention of calcium overload in mitochondria under pathophysiological conditions is discussed.</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|abstract=Mitochondria are capable of synchronized oscillations in many variables, but the underlying mechanisms are still unclear. In this study, we demonstrated that rat liver mitochondria, when exposed to a pulse of Sr2+ ions in the presence of valinomycin (a potassium ionophore) and cyclosporin A (a specific inhibitor of the permeability transition pore complex) under hypotonia, showed prolonged oscillations in K+ and Sr2+ fluxes, membrane potential, pH, matrix volume, rates of oxygen consumption and H2O2 formation. The dynamic changes in the rate of H2O2 production were in a reciprocal relationship with the respiration rate and in a direct relationship with the mitochondrial membrane potential and other indicators studied. The pre-incubation of mitochondria with Ca2+(Sr2+)-dependent phospholipase A2 inhibitors considerably suppressed the accumulation of free fatty acids, including palmitic and stearic acids, and all spontaneous Sr2+-induced cyclic changes. These data suggest that the mechanism of ion efflux from mitochondria is related to the opening of short-living pores, which can be caused by the formation of complexes between Sr2+(Ca2+) and endogenous long-chain saturated fatty acids (mainly, palmitic acid) that accumulate due to the activation of phospholipase A2 by the ions. A possible role for transient palmitate/Ca2+(Sr2+)-induced pores in the maintenance of ion homeostasis and the prevention of calcium overload in mitochondria under pathophysiological conditions is discussed.</div></td></tr>
<tr><td colspan="2"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">|keywords=Cyclosporin A, Cyclosporin A-independent palmitate/Ca2+-induced permeability transition pore, Ion oscillations, Lipid pore, Mitochondria, Mitochondrial permeability transition, Palmitic acid, Phospholipase A2</ins></div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|editor=[[Plangger M]]</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|editor=[[Plangger M]]</div></td></tr>
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Plangger Mario
https://wiki.oroboros.at/index.php?title=Belosludtseva_2022_Membranes_(Basel)&diff=231044&oldid=prev
Plangger Mario: Created page with "{{Publication |title=Belosludtseva NV, Pavlik LL, Belosludtsev KN, Saris NL, Shigaeva MI, Mironova GD (2022) The short-term opening of cyclosporin A-independent palmitate/Sr <..."
2022-08-08T13:58:46Z
<p>Created page with "{{Publication |title=Belosludtseva NV, Pavlik LL, Belosludtsev KN, Saris NL, Shigaeva MI, Mironova GD (2022) The short-term opening of cyclosporin A-independent palmitate/Sr <..."</p>
<p><b>New page</b></p><div>{{Publication<br />
|title=Belosludtseva NV, Pavlik LL, Belosludtsev KN, Saris NL, Shigaeva MI, Mironova GD (2022) The short-term opening of cyclosporin A-independent palmitate/Sr <sup>2+</sup>-induced pore can underlie ion efflux in the oscillatory mode of functioning of rat liver mitochondria.<br />
|info=Membranes (Basel) 12:667. [https://www.ncbi.nlm.nih.gov/pubmed/35877870 PMID: 35877870 Open Access]<br />
|authors=Belosludtseva NV, Pavlik LL, Belosludtsev KN, Saris NL, Shigaeva MI, Mironova GD<br />
|year=2022<br />
|journal=Membranes (Basel)<br />
|abstract=Mitochondria are capable of synchronized oscillations in many variables, but the underlying mechanisms are still unclear. In this study, we demonstrated that rat liver mitochondria, when exposed to a pulse of Sr2+ ions in the presence of valinomycin (a potassium ionophore) and cyclosporin A (a specific inhibitor of the permeability transition pore complex) under hypotonia, showed prolonged oscillations in K+ and Sr2+ fluxes, membrane potential, pH, matrix volume, rates of oxygen consumption and H2O2 formation. The dynamic changes in the rate of H2O2 production were in a reciprocal relationship with the respiration rate and in a direct relationship with the mitochondrial membrane potential and other indicators studied. The pre-incubation of mitochondria with Ca2+(Sr2+)-dependent phospholipase A2 inhibitors considerably suppressed the accumulation of free fatty acids, including palmitic and stearic acids, and all spontaneous Sr2+-induced cyclic changes. These data suggest that the mechanism of ion efflux from mitochondria is related to the opening of short-living pores, which can be caused by the formation of complexes between Sr2+(Ca2+) and endogenous long-chain saturated fatty acids (mainly, palmitic acid) that accumulate due to the activation of phospholipase A2 by the ions. A possible role for transient palmitate/Ca2+(Sr2+)-induced pores in the maintenance of ion homeostasis and the prevention of calcium overload in mitochondria under pathophysiological conditions is discussed.<br />
|editor=[[Plangger M]]<br />
}}<br />
{{Labeling<br />
|area=Respiration<br />
|instruments=Oxygraph-2k<br />
|additional=2022-08<br />
}}</div>
Plangger Mario