Bioblast alert 2015

Bioblast alert

Bioblast alert - in preparation

Bioblast alert #02

O2k-Workshop IOC64 - Centre for Cellular and Molecular Biology, Hyderabad, India: 2011-12-08.

O2k-Publication: Heart failure and mitochondrial dysfunction. Increased OXPHOS capacity after cold preservation of the human heart – a paradox resolved by a dyscoupling mechanism.

Lemieux H, Semsroth S, Antretter H, Hoefer D, Gnaiger E (2011) Mitochondrial respiratory control and early defects of oxidative phosphorylation in the failing human heart. Int. J. Biochem. Cell Biol. 43: 1729–1738. -- Discussion: Complementary results on the PGC-1a pathway.

Scientific service: Tissue storage on ice is possible for limited periods of time (several hours) with preservation of mitochondrial respiratory function, as evaluated with SUIT protocols.

Term of the week: Uncoupled, non-coupled, dyscoupled - a hallmark of high-resolution respirometry is a matching high-resolution terminology.

Evaluate an example of low-resolution terminology: Uncoupling was studied in stressed cells by measurement of coupled respiration, evaluation of uncoupled respiration after inhibition with oligomycin, and FCCP titration to quantify uncoupled respiration.

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O2k-Publication: Protocols for HRR: from intact cells to permeabilized muscle fibres

Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopisies of human muscle. Methods Mol. Biol. 810: 25-58.

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O2k-Publication: Fluorescence in the O2k

Hickey AJ, Renshaw GM, Speers-Roesch B, Richards JG, Wang Y, Farrell AP, Brauner CJ (2011) A radical approach to beating hypoxia: depressed free radical release from heart fibres of the hypoxia-tolerant epaulette shark (Hemiscyllum ocellatum). J. Comp. Physiol. B.[Epub ahead of print]

Anderson EJ, Rodriguez E, Anderson CA, Thayne K, Chitwood WR, Kypson AP (2011) Increased propensity for cell death in diabetic human heart is mediated by mitochondrial-dependent pathways. Am. J. Physiol. Heart Circ. Physiol. 300: H118-124.

MiPNet Reference Laboratory: NZ Auckland Hickey AJ - 6 O2k, guest lecturer at IOC66, joint project (Marsden), guest tutor at IOC59.

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O2k-Publication: Efficiency, basal respiration, and oxygen kinetics in human muscle or Mitochondrial efficiency and P/O flux ratios: new perspectives in mitochondrial physiology (see Journal club);

Larsen FJ, Schiffer TA, Borniquel S, Sahlin K, Ekblom B, Lundberg JO, Weitzberg E (2011) Dietary inorganic nitrate improves mitochondrial efficiency in humans. Cell Metab. 13: 149-159.

Larsen FJ, Schiffer TA, Sahlin K, Ekblom B, Weitzberg E, Lundberg JO (2011) Mitochondrial oxygen affinity predicts basal metabolic rate in humans. FASEB J. 25: 2843-2852.

Before alert, ask to join as MiPNet Reference Lab, and present new MiPNet Reference Lab
Term: efficiency versus P/O ratio

Bioblast alert - previous

Bioblast alert #01 (2011-11-11)

O2k-Fluorimetry Workshop IOC66: 2012-03-15 to 16. OROBOROS INSTRUMENTS, Innsbruck, Austria. The first series of the O2k-Fluorescence Module will be delivered in April 2012, for orders received by end of January 2012.
O2k-Publication: A key question on the separation of qualitative versus quantitative change of mitochondrial function is addressed in a new O2k-Publication. Does training induce mild uncoupling? Are sedentary humans healthy controls?

Pesta D, Hoppel F, Macek C, Messner H, Faulhaber M, Kobel C, Parson W, Burtscher M, Schocke M, Gnaiger E (2011) Similar qualitative and quantitative changes of mitochondrial respiration following strength and endurance training in normoxia and hypoxia in sedentary humans. Am. J. Physiol. Regul. Integr. Comp. Physiol. 301: R1078–R1087.

Scientific service: ETS capacity plays a key role in such analyses, which cannot be achieved quantitatively without careful uncoupler titrations.
Term of the month: Basal respiration - a hallmark of high-resolution respirometry is a matching high-resolution terminology.