Bioblast alert 2015

Bioblast alert

Bioblast alert messages are prepared by the Bioblast editorial board to draw attention to specific topics on the Bioblast wiki. Bioblast alert is for information purposes only, it is not intended to be an advertisement. Topics submitted by the general scientific community are very welcome.

Bioblast alert 2011(04)

Best Bioblast-wishes for a fulfilling, high-quality New Year 2012
Bioblast editorial board - Why a wiki editorial board?
O2k-Publication: SUIT Protocols for HRR: from intact cells to permeabilized muscle fibres

Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopisies of human muscle. Methods Mol Biol 810: 25-58.

Bioblast alert 2011(03): 2011-11-24

Fluorometry combined with high-resolution respirometry: Light guide inserted into the chamber through a black PEEK stopper^[1], or optical probe operated through the O2k-glass window^[2].

Fluorometry versus fluorimetry - term of the week. >>>New List of fluorometry terms - high-resolution terminology.

MiPNet Laboratory of the week - Auckland, New Zealand: Anthony J Hickey.

Bioblast alert 2011(02): 2011-11-17

O2k-Workshop IOC64 - Centre for Cellular and Molecular Biology, Hyderabad, India: 2011-12-08.

O2k-Publication: Heart failure and mitochondrial dysfunction. Increased OXPHOS capacity after cold preservation of the human heart – a paradox resolved by a dyscoupling mechanism.

Lemieux H, Semsroth S, Antretter H, Hoefer D, Gnaiger E (2011) Mitochondrial respiratory control and early defects of oxidative phosphorylation in the failing human heart. Int J Biochem Cell Biol 43: 1729–1738. >>> Discussion: Complementary results on the PGC-1a pathway.

Tissue storage on ice is possible for limited periods of time (several hours) with preservation of mitochondrial respiratory function, as evaluated with SUIT protocols. (Scientific service)

Uncoupled, non-coupled, dyscoupled - term of the week: a hallmark of high-resolution respirometry is a matching high-resolution terminology.

Evaluate an example of low-resolution terminology: Uncoupling was studied in stressed cells by measurement of coupled respiration, evaluation of uncoupled respiration after inhibition with oligomycin, and FCCP titration to quantify uncoupled respiration.

Bioblast alert 2011(01): 2011-11-11

O2k-Fluorometry Workshop IOC66: 2012-03-15 to 16. OROBOROS INSTRUMENTS, Innsbruck, Austria. The first series of the O2k-Fluorescence Module will be delivered in April 2012, for orders received by end of January 2012.
O2k-Publication: A key question on the separation of qualitative versus quantitative change of mitochondrial function is addressed in a new O2k-Publication. Does training induce mild uncoupling? Are sedentary humans healthy controls?

Pesta D, Hoppel F, Macek C, Messner H, Faulhaber M, Kobel C, Parson W, Burtscher M, Schocke M, Gnaiger E (2011) Similar qualitative and quantitative changes of mitochondrial respiration following strength and endurance training in normoxia and hypoxia in sedentary humans. Am J Physiol Regul Integr Comp Physiol 301: R1078–R1087.

ETS capacity plays a key role in such analyses, which cannot be achieved quantitatively without careful uncoupler titrations. (Scientific service)
Basal respiration - term of the month: a hallmark of high-resolution respirometry is a matching high-resolution terminology.

[1] Anderson EJ, Rodriguez E, Anderson CA, Thayne K, Chitwood WR, Kypson AP (2011) Increased propensity for cell death in diabetic human heart is mediated by mitochondrial-dependent pathways. Am J Physiol Heart Circ Physiol 300: H118-124.

[2] Hickey AJ, Renshaw GM, Speers-Roesch B, Richards JG, Wang Y, Farrell AP, Brauner CJ (2011) A radical approach to beating hypoxia: depressed free radical release from heart fibres of the hypoxia-tolerant epaulette shark (Hemiscyllum ocellatum). J Comp Physiol B [Epub ahead of print]

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