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Difference between revisions of "Biochemical threshold effect"

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[[File:NS-threshold.jpg|400px|thumb|Biochemical threshold plot for convergent CI&II-linked (full line, left) and CI-linked pathway flux based on electron gating (dotted line, right). An enzymatic defect of a single step (CIV) exerts no or little effect on pathway flux (horizontal lines) up to a threshold. Beyond the threshold a linear slope is obtained, which is extrapolated to the apparent excess capacity of the single step ([[Gnaiger 2014 MitoPathways]], Fig. 1.6, edited 2016-02-25).]]
[[File:NS-threshold.jpg|400px|thumb|Biochemical threshold plot for convergent NS (CI<small>&</small>II-linked; full line, left) and N (CI-linked) pathway flux based on electron gating (dotted line, right). An enzymatic defect of a single step (CIV) exerts no or little effect on N-pathway and NS-pathway flux (horizontal lines) up to a threshold. Beyond the threshold a linear slope is obtained, which is extrapolated to the apparent excess capacity of the single step. The physiological threshold is overestimated for N-pathway flux, which is restricted by electron gating ([[Gnaiger 2014 MitoPathways]], Fig. 1.6, edited 2016-02-25).]]

Revision as of 14:07, 25 February 2016


high-resolution terminology - matching measurements at high-resolution


Biochemical threshold effect

Description

Due to threshold effects, even a large defect diminishing the velocity of an individual enzyme results in only minor changes of pathway flux.


Reference: Gnaiger 2014 MitoPathways



MitoPedia methods: Respirometry 


MitoPedia O2k and high-resolution respirometry: "SUIT protocol" is not in the list (O2k hardware, DatLab, Oroboros QM, O2k-Open Support, O2k-Respirometry, O2k-FluoRespirometry) of allowed values for the "MitoPedia O2k and high-resolution respirometry" property. SUIT protocol"SUIT protocol" is not in the list (O2k hardware, DatLab, Oroboros QM, O2k-Open Support, O2k-Respirometry, O2k-FluoRespirometry) of allowed values for the "MitoPedia O2k and high-resolution respirometry" property. 


MitoPedia topics: Enzyme 

Biochemical threshold plot for convergent NS (CI&II-linked; full line, left) and N (CI-linked) pathway flux based on electron gating (dotted line, right). An enzymatic defect of a single step (CIV) exerts no or little effect on N-pathway and NS-pathway flux (horizontal lines) up to a threshold. Beyond the threshold a linear slope is obtained, which is extrapolated to the apparent excess capacity of the single step. The physiological threshold is overestimated for N-pathway flux, which is restricted by electron gating (Gnaiger 2014 MitoPathways, Fig. 1.6, edited 2016-02-25).