Biochemical threshold effect

high-resolution terminology - matching measurements at high-resolution

Biochemical threshold effect

Description

Due to threshold effects, even a large defect diminishing the velocity of an individual enzyme results in only minor changes of pathway flux.

Reference: Gnaiger 2014 MitoPathways

MitoPedia methods: Respirometry 

MitoPedia O2k and high-resolution respirometry: "SUIT protocol" is not in the list (O2k hardware, DatLab, Oroboros QM, O2k-Open Support, O2k-Respirometry, O2k-FluoRespirometry) of allowed values for the "MitoPedia O2k and high-resolution respirometry" property. SUIT protocol"SUIT protocol" is not in the list (O2k hardware, DatLab, Oroboros QM, O2k-Open Support, O2k-Respirometry, O2k-FluoRespirometry) of allowed values for the "MitoPedia O2k and high-resolution respirometry" property. 

MitoPedia topics: Enzyme 

Biochemical threshold plot for convergent CI&II-linked (full line, left) and CI-linked pathway flux based on electron gating (dotted line, right). An enzymatic defect of a single step (CIV) exerts no or little effect on pathway flux (horizontal lines) up to a threshold. Beyond the threshold a linear slope is obtained, which is extrapolated to the apparent excess capacity of the single step (Gnaiger 2014 MitoPathways, Fig. 1.6, edited 2016-02-25).