Boyle 2012 Brain Res: Difference between revisions
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| | |diseases=Aging; senescence, Alzheimer's, Neurodegenerative | ||
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Revision as of 19:43, 7 August 2013
Boyle JP, Hettiarachchi NT, Wilkinson JA, Pearson HA, Scragg JL, Lendon C, Al-Owais MM, Kim CB, Myers DM, Warburton P, Peers C (2012) Cellular consequences of the expression of Alzheimer's disease-causing presenilin 1 mutations in human neuroblastoma (SH-SY5Y) cells. Brain Res 1443: 75-88 |
Boyle JP, Hettiarachchi NT, Wilkinson JA, Pearson HA, Scragg JL, Lendon C, Al-Owais MM, Kim CB, Myers DM, Warburton P, Peers C (2012) Brain Res
Abstract: Mutations in the presenilin 1 (PS1) gene lead to early-onset Alzheimer's disease with the S170F mutation causing the earliest reported age of onset. Expression of this, and other PS1 mutations, in SH-SY5Y cells resulted in significant loss of cellular viability compared to control cells. Basal Ca2+ concentrations in PS1 mutants were never lower than controls and prolonged incubation in Ca2+-free solutions did not deplete Ca2+ stores, demonstrating there was no difference in Ca2+ leak from endoplasmic reticulum (ER) stores in PS1 mutants. Peak muscarine-evoked rises of [Ca2+](i) were variable, but the integrals were not significantly different, suggesting, while kinetics of Ca2+ store release might be affected in PS1 mutants, store size was similar. However, when Ca2+-ATPase activity was irreversibly inhibited with thapsigargin, the S170F and ฮE9 cells showed larger capacitative calcium entry indicating a direct effect on Ca2+ influx pathways. There was no significant effect of any of the mutations on mitochondrial respiration. Amyloid ฮฒ(Aฮฒ(1-40)) secretion was reduced, and Aฮฒ(1-42) secretion increased in the S170F cells resulting in a very large increase in the Aฮฒ42/40 ratio. This, rather than any potential disruption of ER Ca2+ stores, is likely to explain the extreme pathology of this mutant. โข Keywords: Alzheimer's disease, Presenilin 1 (PS1), Amyloid ฮฒ Aฮฒ(1-40) and Aฮฒ(1-42), Endoplasmic reticulum
โข O2k-Network Lab: UK Leeds Peers C
Labels:
Pathology: Aging; senescence"Aging; senescence" is not in the list (Aging;senescence, Alzheimer's, Autism, Cancer, Cardiovascular, COPD, Diabetes, Inherited, Infectious, Myopathy, ...) of allowed values for the "Diseases" property., Alzheimer's, Neurodegenerative
Organism: Human
Preparation: Intact cells
Regulation: Respiration"Respiration" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property. Coupling state: ROUTINE
HRR: Oxygraph-2k, Ca