Brand 1976 Proc Natl Acad Sci USA: Difference between revisions
No edit summary |
Bader Helga (talk | contribs) No edit summary ย |
||
(One intermediate revision by the same user not shown) | |||
Line 1: | Line 1: | ||
{{Publication | {{Publication | ||
|title=Brand MD, Reynafarje B, Lehninger AL (1976) Stoichiometric relationship between energy-dependent proton ejection and electron transport in mitochondria. Proc Natl Acad Sci U S A 73: 437- | |title=Brand MD, Reynafarje B, Lehninger AL (1976) Stoichiometric relationship between energy-dependent proton ejection and electron transport in mitochondria. Proc Natl Acad Sci U S A 73:437-41. | ||
|info=[http://www.ncbi.nlm.nih.gov/pubmed?term=Stoichiometric%20relationship%20between%20energy-dependent%20proton%20ejection%20and%20electron%20transport%20in%20mitochondria PMID:1061146 Open Access] | |info=[http://www.ncbi.nlm.nih.gov/pubmed?term=Stoichiometric%20relationship%20between%20energy-dependent%20proton%20ejection%20and%20electron%20transport%20in%20mitochondria PMID:1061146 Open Access] | ||
|authors=Brand MD, Reynafarje B, Lehninger AL | |authors=Brand MD, Reynafarje B, Lehninger AL | ||
Line 11: | Line 11: | ||
Abstract Continued in Free Text | Abstract Continued in Free Text | ||
|keywords= | |keywords=Energy-dependent proton ejection, Electron transport | ||
}} | }} | ||
{{Labeling | {{Labeling | ||
Line 17: | Line 17: | ||
|organism=Rat | |organism=Rat | ||
|tissues=Liver | |tissues=Liver | ||
|preparations=Isolated | |preparations=Isolated mitochondria | ||
|topics=Coupling efficiency;uncoupling, Ion;substrate transport, pH | |topics=Coupling efficiency;uncoupling, Ion;substrate transport, pH | ||
|couplingstates=OXPHOS | |couplingstates=OXPHOS |
Latest revision as of 11:13, 27 May 2015
Brand MD, Reynafarje B, Lehninger AL (1976) Stoichiometric relationship between energy-dependent proton ejection and electron transport in mitochondria. Proc Natl Acad Sci U S A 73:437-41. |
Brand MD, Reynafarje B, Lehninger AL (1976) Proc Natl Acad Sci U S A
Abstract: The number of protons ejected during electron transport per pair of electrons per energy-conserving site (the H+/site ratio) was measured in rat liver mitochondria by three different methods under conditions in which transmembrane movements of endogenous phosphate were minized or eliminated.
- In the Ca2+ pulse method, between 3.5 and 4.0 molecules of 3-hydroxybutyrate and 1.75 to 2.0 Ca2+ ions were accumulated per 2 e- per site during Ca2+ induced electron transport in the presence of rotenone, when measured under conditions in which movements of endogenous phosphate were negligible. Since entry of 3-hydroxybutyrate requires its protonation to the free acid these data correspond to an H+/site ratio of 3.5-4.0
- In the oxygen pulse method addition of known amounts of oxygen to anaerobic mitochondria in the presence of substrate yielded H+/site ratios of 3.0 when phosphate transport was eliminated by addition of N-ethylmaleimide or by anaerobic washing to remove endogenous phosphate. In the absence of such measures the observed H+/site ratio was 2.0.
- In the reductant pulse method measurement of the initial steady rates of H+ ejection and oxygen consumption by mitochondria in an aerobic medium after addition of substrate gave H+/site near 4.0 in the presence of N-ethylmaleimide; in the absence of the inhibitor the observed ratio was only 2.0.
Abstract Continued in Free Text โข Keywords: Energy-dependent proton ejection, Electron transport
Labels: MiParea: Respiration
Organism: Rat
Tissue;cell: Liver
Preparation: Isolated mitochondria
Regulation: Coupling efficiency;uncoupling, Ion;substrate transport, pH Coupling state: OXPHOS
Made history
- Abstract Cont.
These and other experiments reported indicate that the values of 2.0 earlier obtained for the H+/site ratio by Mitchell and Moyle [Biochem J. (1967) 105, 1147-1162] and others were underestimates due to the unrecognized masking of H+ ejection by movements of endogenous phosphate. The results presented here show that the H+/site ratio of mitochondrial electron transport is at least 3.0 and may be as high as 4.0.