Cookies help us deliver our services. By using our services, you agree to our use of cookies. More information

Difference between revisions of "Burtscher 2015 Mitochondrion"

From Bioblast
Line 22: Line 22:
|diseases=Neurodegenerative
|diseases=Neurodegenerative
|couplingstates=LEAK, OXPHOS, ETS
|couplingstates=LEAK, OXPHOS, ETS
|substratestates=CI, CII, CIV, CI&II, ROX
|pathways=N, S, CIV, NS, ROX
|instruments=Oxygraph-2k
|instruments=Oxygraph-2k
|additional=PBI-Shredder, MitoFit news
|additional=PBI-Shredder, MitoFit news

Revision as of 14:42, 7 November 2016

Publications in the MiPMap
Burtscher J, Zangrandi L, Schwarzer C, Gnaiger E (2015) Differences in mitochondrial function in homogenated samples from healthy and epileptic specific brain tissues revealed by high-resolution respirometry. Mitochondrion 25:104-12.

ยป PMID: 26516105 Open Access

Burtscher J, Zangrandi L, Schwarzer C, Gnaiger E (2015) Mitochondrion

Abstract: Mitochondrial dysfunction and oxidative stress are strongly implicated in neurodegenerative diseases and epilepsy. Strikingly, neurodegenerative diseases show regional specificity in vulnerability and follow distinct patterns of neuronal loss. A challenge is to understand, why mitochondria fail in particular brain regions under specific pathological conditions. A potential explanation could be provided by regional or cellular specificity of mitochondrial function.

We applied high-resolution respirometry to analyze the integrated Complex I- and II (CI and CII)-linked respiration, the activity of Complex IV, and the combined CI&II-linked oxidative phosphorylation (OXPHOS)- and electron-transfer system (ETS)-capacity in microsamples obtained from distinct regions of the mouse brain. We compared different approaches to assess mitochondrial density and suggest flux control ratios as a valid method to normalize respiration to mitochondrial density.

This approach revealed significant differences of CI- and CII-linked OXPHOS capacity and coupling control between motor cortex, striatum, hippocampus and pons of naรฏve mice. CI-linked respiration was highest in motor cortex, while CII-linked respiration predominated in the striatum. To investigate if this method could also determine differences in normal and disease states within the same brain region, we compared hippocampal homogenates in a chronic epilepsy model. Three weeks after stereotaxic injection of kainate, there was a down-regulation of CI- and upregulation of CII-linked respiration in the resulting epileptic ipsilateral hippocampus compared to the contralateral one.

In summary, respirometric OXPHOS analysis provides a very sensitive diagnostic approach using small amounts of distinct brain tissues. In a single assay, information is obtained on numerous OXPHOS parameters as indicators of tissue-specific mitochondrial performance. โ€ข Keywords: Neurodegeneration, Epilepsy, Mitochondria, Brain regions, Respirometry

โ€ข O2k-Network Lab: AT Innsbruck Gnaiger E, AT Innsbruck OROBOROS


Labels: MiParea: Respiration, Instruments;methods, mt-Biogenesis;mt-density, Pharmacology;toxicology  Pathology: Neurodegenerative 

Organism: Mouse  Tissue;cell: Nervous system  Preparation: Permeabilized tissue, Homogenate 


Coupling state: LEAK, OXPHOS, ETS"ETS" is not in the list (LEAK, ROUTINE, OXPHOS, ET) of allowed values for the "Coupling states" property.  Pathway: N, S, CIV, NS, ROX  HRR: Oxygraph-2k 

PBI-Shredder, MitoFit news 

MitoFit

MitoFit news 2015#17