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COST Action CA15203 (2016-2021): MitoEAGLE
Evolution-Age-Gender-Lifestyle-Environment: mitochondrial fitness mapping
Calisto F
MitoPedia topics: EAGLE
COST: Member
Name | Calisto Filipa, PhD student |
---|---|
Institution | Biological Energy Transduction Group
ITQB-UNL |
Address | Av. da República
Estação Agronómica Nacional, 2780-157 |
City | Oeiras |
State/Province | |
Country | Portugal |
[email protected] | |
Weblink | http://www.itqb.unl.pt/bet |
O2k-Network Lab |
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MitoEAGLE Short-Term Scientific Mission
- Work Plan summary
- I would like to apply to a short-term scientific mission grant, which will allow me to understand the electron transfer pathway and the proton translocation mechanism of Alternative Complex III. I am a PhD student at ITQB in the Biological Energy Transduction group under the supervision of Manuela M. Pereira. I am dedicated to the study of the Alternative Complex III (ACIII), a protein from bacteria respiratory chain, which has the same quinol:cytochrome c oxidoreductase activity as the cytochrome bc1 complex. Recently, I published as first coauthor an article on the structural characterization of ACIII (Nat Commun. 2018 doi: 10.1038/s41467-018-04141-8) where we identified two putative proton pathways and one quinol-binding. These results provide the first direct evidence that ACIII operates by a redox-driven proton translocation mechanism, totally unrelated to the Q-cycle of cytochrome bc1 complex. In order to extend this characterization, I would like to test the proton pump activity of ACIII in liposomes, using fluorescence and advance membrane protein purification/reconstitution methodologies pioneered in Dr Duncan G.G. McMillan Lab at the Department of Biotechnology, Delft University of Technology. Thank you for considering my application. Sincerely, Filipa Calisto
- Work Plan summary