Difference between revisions of "Chinopoulos 2011 Methods Mol Biol"
(Created page with "{{Publication |title=Chinopoulos C, Zhang SF, Thomas B, Ten V, Starkov AA (2011) Isolation and functional assessment of mitochondria from small amounts of mouse brain tissue....") Β |
|||
| Line 11: | Line 11: | ||
}} | }} | ||
{{Labeling | {{Labeling | ||
|area=Respiration | |area=Respiration, Instruments;methods | ||
|enzymes=Adenine nucleotide translocase, Complex V;ATP synthase | |enzymes=Adenine nucleotide translocase, Complex V;ATP synthase | ||
|topics=ATP production, mt-Membrane potential | |topics=ATP production, mt-Membrane potential | ||
|instruments=Theory, O2k-FluoRespirometer | |instruments=Theory, O2k-FluoRespirometer | ||
}} | }} | ||
Revision as of 17:32, 6 February 2018
| Chinopoulos C, Zhang SF, Thomas B, Ten V, Starkov AA (2011) Isolation and functional assessment of mitochondria from small amounts of mouse brain tissue. Methods Mol Biol 793:311-24. |
Chinopoulos C, Zhang SF, Thomas B, Ten V, Starkov AA (2011) Methods Mol Biol
Abstract: Recent discoveries have brought mitochondria functions in focus of the neuroscience research community and greatly stimulated the demand for approaches to study mitochondria dysfunction in neurodegenerative diseases. Many mouse disease models have been generated, but studying mitochondria isolated from individual mouse brain regions is a challenge because of small amount of the available brain tissue. Conventional techniques for isolation and purification of mitochondria from mouse brain subregions, such as ventral midbrain, hippocampus, or striatum, require pooling brain tissue from six to nine animals for a single mitochondrial preparation. Working with pooled tissue significantly decreases the quality of data because of the time required to dissect several brains. It also greatly increases the labor intensity and the cost of experiments as several animals are required per single data point. We describe a method for isolation of brain mitochondria from mouse striata or other 7-12 mg brain samples. The method utilizes a refrigerated table-top microtube centrifuge, and produces research grade quality mitochondria in amounts sufficient for performing multiple enzymatic and functional assays, thereby eliminating the necessity for pooling mouse brain tissue. We also include a method of measuring ADP-ATP exchange rate as a function of mitochondrial membrane potential (ΞΞ¨m) in small amounts of isolated mitochondria, adapted to a plate reader format. β’ Keywords: Adenine nucleotide translocase; Adenine nucleotide carrier; Systems biology β’ Bioblast editor: Sumbalova Z β’ O2k-Network Lab: HU Budapest Chinopoulos C
Labels: MiParea: Respiration, Instruments;methods
Enzyme: Adenine nucleotide translocase, Complex V;ATP synthase Regulation: ATP production, mt-Membrane potential
HRR: Theory, O2k-FluoRespirometer"O2k-FluoRespirometer" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property.
