Coenzyme Q

high-resolution terminology - matching measurements at high-resolution

Coenzyme Q

Description

Coenzyme Q, redox system (oxidized ubiquinone, partially reduced semiquinone, fully reduced ubiquinol) of the ET-pathway.

More details » Q-junction.

Abbreviation: Q, CoQ

Reference: Gnaiger 2019 MitoPathways

Application in HRR

Q2 : Coenzyme Q2 (Sigma Aldrich, C8081, MW: 318.41; 2,3-Dimethoxy-5-methyl-6-geranyl-1,4-benzoquinone, Ubiquinone-2, oxidized form)

Caution: Light sensitive (store solution in a dark glass vial).

Preparation of 10 mM stock solution (dissolved in ethanol) (to run cyclic voltammetry)

1. Dissolve one commercial vial of Q2 (2 mg) Q2 in 628 µL 99.9% ethanol.
2. Divide into 50 µL aliquots in dark glass vials.
3. Store frozen at -20 °C.

Preparation of 1 mM stock solution (to measure the Q redox ratio)

1. Dilute 50 µL of the 10 mM stock with 450 µL 99.9% ethanol.
2. Divide into 50 µL aliquots in dark glass vials.
3. Store frozen at -20 °C.

O2k manual titrations MiPNet09.12 O2k-Titrations

Q redox ratio

• Titration volume: 2 µL of the 1 mM stock solution using a 10 µL syringe (2 mL O2k-Chamber).
• Final concentration: 1 µM

Cyclic voltammetry

• Titration volume: 6 µL of the 10 mM stock solution using a 10 µL syringe (2 mL O2k-Chamber).
• Final concentration: 30 µM

MitoPedia topics: Enzyme, Substrate and metabolite