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Difference between revisions of "Complex II-linked substrate state"

From Bioblast
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# [[O2k-Protocols]]: [[MiPNet12.11 MitoRespiration]]
# [[O2k-Protocols]]: [[MiPNet12.11 MitoRespiration]]
# Comparison with CI-CI<small>&</small>II-CII SUIT protocol: [[Votion 2012 PLoS One]]
# Comparison with CI-CI<small>&</small>II-CII SUIT protocol: [[Votion 2012 PLoS One]]
# [[SUIT-RP1]]_'''7Rot''': 1PM 2D 2c (2NADH) 3U 4Oct 5G 6S '''<u>7Rot</u>''' 8Gp 9Ama 10Tm 11Azd
# [[SUIT RP1]]_'''7Rot''': 1PM 2D 2c (2NADH) 3U 4Oct 5G 6S '''<u>7Rot</u>''' 8Gp 9Ama 10Tm 11Azd





Revision as of 11:53, 20 July 2016


high-resolution terminology - matching measurements at high-resolution


Complex II-linked substrate state

Description

CII-linked; SRot: Succinate (S) supports electron flux through Complex II via flavin adenine dinucleotide (FADH2) into the membrane-bound electron transfer system. Inhibition of Complex I by rotenone (Rot; or amytal, piericidine) prevents accumulation of oxaloacetate which is a potent inhibitor of succinate dehydrogenase. After inhibition of CI by rotenone, the NADH-linked dehydrogenases become inhibited by the redox shift from NAD+ to NADH. Succinate dehydrogenase is activated by succinate and ATP, which explains in part the time-dependent increase of respiration in isolated mitochondria after addition of rotenone (first), succinate and ADP.

The Complex II-linked substrate state is induced in mt-preparations by addition of succinate&rotenone (Complex I inhibitor). Succinate is the direct substrate of Complex II (succinate dehydrogenase). In CII-linked respiration, only Complex III and Complex IV are involved in pumping protons from the matrix (P-phase) to the N-phase with a ~P/O ratio of 1.75 (P/O2 = 3.5).

Abbreviation: CII, CII-linked, SRot, S

Reference: Gnaiger 2014 MitoPathways - Chapter 4.1, Gnaiger 2000 Proc Natl Acad Sci U S A


MitoPedia concepts: Respiratory state, SUIT state 





MitoPedia topics: Substrate and metabolite 

Succinate+rotenone, S(Rot). From Gnaiger 2014 MitoPathways.

SRot(L)

  1. ~P/O2 ratio: Gnaiger 2000 Proc Natl Acad Sci U S A
  2. O2k-Protocols: MiPNet12.11 MitoRespiration
  3. Comparison with S alone, CI&II, and H2O2 production: Makrecka-Kuka 2015 Biomolecules


SRot(P)

  1. ~P/O2 ratio: Gnaiger 2000 Proc Natl Acad Sci U S A
  2. O2k-Protocols: MiPNet12.11 MitoRespiration
  3. Comparison with S alone CI&II, and H2O2 production: Makrecka-Kuka 2015 Biomolecules


SRot(E)

  1. O2k-Protocols: MiPNet12.11 MitoRespiration
  2. Comparison with CI-CI&II-CII SUIT protocol: Votion 2012 PLoS One
  3. SUIT RP1_7Rot: 1PM 2D 2c (2NADH) 3U 4Oct 5G 6S 7Rot 8Gp 9Ama 10Tm 11Azd


List of publications: CII-linked substrate state

This list does not distinguish between substrate states for Succinate alone (without Rot) and SRot.