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Difference between revisions of "Complex II-linked substrate state"

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{{MitoPedia
{{MitoPedia
|abbr=CII, CII-linked, SRot, S
|abbr=SRot, S
|description='''CII-linked; SRot''': '''[[Succinate]]''' (S) supports electron flux through [[Complex II]] via flavin adenine dinucleotide (FADH2) into the membrane-bound [[electron transfer system]]. Inhibition of Complex I by '''[[rotenone]]''' (Rot; or amytal, piericidine) prevents accumulation of [[Oxaloacetate|oxaloacetate]] which is a potent inhibitor of [[succinate dehydrogenase]]. After inhibition of CI by [[rotenone]], the NADH-linked dehydrogenases become inhibited by the redox shift from NAD<sup>+</sup> to NADH. Succinate dehydrogenase is activated by succinate and ATP, which explains in part the time-dependent increase of respiration in isolated mitochondria after addition of rotenone (first), succinate and ADP.
|description=''See'' '''[[S-pathway control state]] (previous: CII-linked)
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The '''Complex II-linked substrate state''' is induced in mt-preparations by addition of [[succinate]]&[[rotenone]] (Complex I inhibitor). Succinate is the direct substrate of [[Complex II]] (succinate dehydrogenase). In CII-linked respiration, only Complex III and Complex IV are involved in pumping protons from the matrix (P-phase) to the N-phase with a ~P/O ratio of 1.75 (P/O<sub>2</sub> = 3.5).
|info=[[Gnaiger 2014 MitoPathways |Gnaiger 2014 MitoPathways - Chapter 4.1]], [[Gnaiger 2000 Proc Natl Acad Sci U S A]]
}}
}}
{{MitoPedia concepts}}
{{MitoPedia concepts
{{MitoPedia methods}}
|mitopedia concept=Respiratory state, SUIT state, Find
{{MitoPedia SUIT
|mitopedia SUIT=SUIT state
}}
}}
{{MitoPedia topics
{{MitoPedia topics
|mitopedia topic=Respiratory state, Substrate and metabolite
|mitopedia topic=EAGLE
}}
[[File:S(Rot).jpg|400px|thumb|Succinate+rotenone, S(Rot). From [[Gnaiger 2014 MitoPathways]].]]
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== SRot(L) ==
# ~P/O2 ratio: [[Gnaiger 2000 Proc Natl Acad Sci U S A]]
# [[O2k-Protocols]]: [[MiPNet12.11 MitoRespiration]]
# Comparison with [[S alone]], CI<small>&</small>II, and H<sub>2</sub>O<sub>2</sub> production: [[Makrecka-Kuka 2015 Biomolecules]]
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== SRot(P) ==
# ~P/O2 ratio: [[Gnaiger 2000 Proc Natl Acad Sci U S A]]
# [[O2k-Protocols]]: [[MiPNet12.11 MitoRespiration]]
# Comparison with [[S alone]] CI<small>&</small>II, and H<sub>2</sub>O<sub>2</sub> production: [[Makrecka-Kuka 2015 Biomolecules]]
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== SRot(E) ==
# [[O2k-Protocols]]: [[MiPNet12.11 MitoRespiration]]
# Comparison with CI-CI<small>&</small>II-CII SUIT protocol: [[Votion 2012 PLoS One]]
# [[SUIT-RP1]]_'''7Rot''': 1PM 2D 2c (2NADH) 3U 4Oct 5G 6S '''<u>7Rot</u>''' 8Gp 9Ama 10Tm 11Azd
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== List of publications: CII-linked substrate state ==
: This list does not distinguish between substrate states for [[Succinate alone]] (without Rot) and SRot.
{{#ask:[[Category:Publications]] [[Substrate states::CII]]
|?Was published in year=Year
|?Has title=Reference
|?Mammal and model
|?Tissue and cell
|?Stress
|?Diseases
|format=broadtable
|limit=5000
|offset=0
|sort=Was published in year
|order=descending
}}
}}

Latest revision as of 06:06, 29 May 2018


high-resolution terminology - matching measurements at high-resolution


Complex II-linked substrate state

Description

See S-pathway control state (previous: CII-linked)

Abbreviation: SRot, S


MitoPedia concepts: Respiratory state, SUIT state, Find 


MitoPedia topics: EAGLE