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Complex II-linked substrate state

From Bioblast


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Complex II-linked substrate state

Description

CII-linked; SRot: Succinate (S) supports electron flux through Complex II via flavin adenine dinucleotide (FADH2) into the membrane-bound electron transfer system. Inhibition of Complex I by rotenone (Rot; or amytal, piericidine) prevents accumulation of oxaloacetate which is a potent inhibitor of succinate dehydrogenase. After inhibition of CI by rotenone, the NADH-linked dehydrogenases become inhibited by the redox shift from NAD+ to NADH. Succinate dehydrogenase is activated by succinate and ATP, which explains in part the time-dependent increase of respiration in isolated mitochondria after addition of rotenone (first), succinate and ADP.

The Complex II-linked substrate state is induced in mt-preparations by addition of succinate&rotenone (Complex I inhibitor). Succinate is the direct substrate of Complex II (succinate dehydrogenase). In CII-linked respiration, only Complex III and Complex IV are involved in pumping protons from the matrix (P-phase) to the N-phase with a ~P/O ratio of 1.75 (P/O2 = 3.5).

Abbreviation: CII, CII-linked, SRot, S

Reference: Gnaiger 2014 MitoPathways - Chapter 4.1, Gnaiger 2000 Proc Natl Acad Sci U S A




Template:MitoPedia SUIT

MitoPedia topics: "Respiratory state" is not in the list (Enzyme, Medium, Inhibitor, Substrate and metabolite, Uncoupler, Sample preparation, Permeabilization agent, EAGLE, MitoGlobal Organizations, MitoGlobal Centres, ...) of allowed values for the "MitoPedia topic" property. Respiratory state"Respiratory state" is not in the list (Enzyme, Medium, Inhibitor, Substrate and metabolite, Uncoupler, Sample preparation, Permeabilization agent, EAGLE, MitoGlobal Organizations, MitoGlobal Centres, ...) of allowed values for the "MitoPedia topic" property., Substrate and metabolite 

Succinate+rotenone, S(Rot). From Gnaiger 2014 MitoPathways.

SRot(L)

  1. ~P/O2 ratio: Gnaiger 2000 Proc Natl Acad Sci U S A
  2. O2k-Protocols: MiPNet12.11 MitoRespiration
  3. Comparison with S alone, CI&II, and H2O2 production: Makrecka-Kuka 2015 Biomolecules


SRot(P)

  1. ~P/O2 ratio: Gnaiger 2000 Proc Natl Acad Sci U S A
  2. O2k-Protocols: MiPNet12.11 MitoRespiration
  3. Comparison with S alone CI&II, and H2O2 production: Makrecka-Kuka 2015 Biomolecules


SRot(E)

  1. O2k-Protocols: MiPNet12.11 MitoRespiration
  2. Comparison with CI-CI&II-CII SUIT protocol: Votion 2012 PLoS One
  3. SUIT-RP1_7Rot: 1PM 2D 2c (2NADH) 3U 4Oct 5G 6S 7Rot 8Gp 9Ama 10Tm 11Azd


List of publications: CII-linked substrate state

This list does not distinguish between substrate states for Succinate alone (without Rot) and SRot.