Difference between revisions of "Coupling-control efficiency"
(Created page with "{{MitoPedia |abbr=''CCC'' |description='''Coupling control capacities''', ''CCC'', are flux control capacities, ''FCC'', at a constant mitochondrial [[s...") Β |
|||
| Line 1: | Line 1: | ||
{{MitoPedia | {{MitoPedia | ||
|abbr=''CCC'' | |abbr=''CCC'' | ||
|description='''Coupling control capacities''', ''CCC'', are [[flux control capacity|flux control capacities]], ''FCC'', at a constant | |description='''Coupling control capacities''', ''CCC'', are [[flux control capacity|flux control capacities]], ''FCC'', at a constant [[ETS-competent substrate state]]. In mitochondrial preparations, there are three well-defined coupling states of respiration, ''L'', ''P'', ''E'' ([[LEAK]], [[OXPHOS]], [[ETS]]). In intact cells, state ''P'' cannot be induced, but a [[ROUTINE]] state of respiration, ''R'', can be measured. Β | ||
1. If the [[metabolic control variable]], ''X'', is an [[uncoupler]], the | 1. If the [[metabolic control variable]], ''X'', is an [[uncoupler]], the reference state ''Z'' is ''E''. Then two [[background state]]s, ''Y'', of coupling control are possible: The uncoupler may act on the ''L'' or ''P'' state in mt-preparations, and on the ''L'' or ''R'' state in intact cells. The corresponding coupling control capacities are: Β | ||
* ''E-L'' coupling control capacity, Ξ''j<sub>E-L</sub>'' = (''E-L'')/''E'' = 1-''L/E'' (mt-preparations and intact cells) | * [[E-L coupling control capacity|''E-L'' coupling control capacity]], Ξ''j<sub>E-L</sub>'' = (''E-L'')/''E'' = 1-''L/E'' (mt-preparations and intact cells) Β | ||
* ''E-P'' coupling control capacity, Ξ''j<sub>E-P</sub>'' = (''E-P'')/''E'' = 1-''P/E'' (mt-preparations) | * [[E-P coupling control capacity|''E-P'' coupling control capacity]], Ξ''j<sub>E-P</sub>'' = (''E-P'')/''E'' = 1-''P/E'' (mt-preparations) Β | ||
* ''E- | * [[E-R coupling control capacity|''E-R'' coupling control capacity]], Ξ''j<sub>E-P</sub>'' = (''E-R'')/''E'' = 1-''R/E'' (intact cells) Β | ||
or ''P'', | 2. If the metablic control variable is stimulation by [[ADP]], D, or release of an inhibitor of phosphorylation of ADP to ATP ([[DT-phosphorylation]]; e.g. -Omy), the reference state ''Z'' is ''P'' in mt-preparations at saturating [[[ADP]]], or ''R'' in intact cells at physiologically controlled steady states of [ADP] and ATP-turnover. The background state ''Y'' is ''L'', and the corresponding coupling control capacities are: | ||
|info=[[Gnaiger | * [[P-L coupling control capacity|''P-L'' coupling control capacity]], Ξ''<sub>jP-L</sub>'' = (''P-L'')/''P'' = 1-''L/P'' (mt-preparations) | ||
* [[R-L coupling control capacity|''R-L'' coupling control capacity]], Ξ''j<sub>R-L</sub>'' = (''R-L'')/''R'' = 1-''L/R'' (intact cells) | |||
Β | |||
|info=[[Gnaiger 2013 Abstract MiP2013]] | |||
}} | }} | ||
{{MitoPedia methods | {{MitoPedia methods | ||
| Line 19: | Line 20: | ||
|mitopedia topic=Respiratory control ratio | |mitopedia topic=Respiratory control ratio | ||
}} | }} | ||
Revision as of 18:04, 4 August 2013
- high-resolution terminology - matching measurements at high-resolution
Coupling-control efficiency
Description
Coupling control capacities, CCC, are flux control capacities, FCC, at a constant ETS-competent substrate state. In mitochondrial preparations, there are three well-defined coupling states of respiration, L, P, E (LEAK, OXPHOS, ETS). In intact cells, state P cannot be induced, but a ROUTINE state of respiration, R, can be measured.
1. If the metabolic control variable, X, is an uncoupler, the reference state Z is E. Then two background states, Y, of coupling control are possible: The uncoupler may act on the L or P state in mt-preparations, and on the L or R state in intact cells. The corresponding coupling control capacities are:
- E-L coupling control capacity, ΞjE-L = (E-L)/E = 1-L/E (mt-preparations and intact cells)
- E-P coupling control capacity, ΞjE-P = (E-P)/E = 1-P/E (mt-preparations)
- E-R coupling control capacity, ΞjE-P = (E-R)/E = 1-R/E (intact cells)
2. If the metablic control variable is stimulation by ADP, D, or release of an inhibitor of phosphorylation of ADP to ATP (DT-phosphorylation; e.g. -Omy), the reference state Z is P in mt-preparations at saturating [[[ADP]]], or R in intact cells at physiologically controlled steady states of [ADP] and ATP-turnover. The background state Y is L, and the corresponding coupling control capacities are:
- P-L coupling control capacity, ΞjP-L = (P-L)/P = 1-L/P (mt-preparations)
- R-L coupling control capacity, ΞjR-L = (R-L)/R = 1-L/R (intact cells)
Abbreviation: CCC
Reference: Gnaiger 2013 Abstract MiP2013
MitoPedia methods:
Respirometry
MitoPedia topics: "Respiratory control ratio" is not in the list (Enzyme, Medium, Inhibitor, Substrate and metabolite, Uncoupler, Sample preparation, Permeabilization agent, EAGLE, MitoGlobal Organizations, MitoGlobal Centres, ...) of allowed values for the "MitoPedia topic" property.
Respiratory control ratio"Respiratory control ratio" is not in the list (Enzyme, Medium, Inhibitor, Substrate and metabolite, Uncoupler, Sample preparation, Permeabilization agent, EAGLE, MitoGlobal Organizations, MitoGlobal Centres, ...) of allowed values for the "MitoPedia topic" property.
