Difference between revisions of "Coupling-control ratio"
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|description='''Coupling control ratios''', ''CCR'', are [[flux control ratio]]s, ''FCR'', at a constant mitochondrial [[substrate state]]. In mitochondrial preparations, there are three well-defined coupling states of respiration, ''L'', ''P'', ''E'' ([[LEAK]], [[OXPHOS]], [[ETS]]). In intact cells, state ''P'' cannot be induced, but a [[ROUTINE]] state of respiration, ''R'', can be measured. The reference state, ''J''<sub>ref</sub>, is defined by taking ''J''<sub>ref</sub> as the maximum flux, i.e. flux in the ETS state, ''E'', such that the lower and upper limits of ''CCR'' are defined as 0.0 and 1.0. Then there are two mitochondrial ''CCR'', [[L/E|''L/E'']] and [[P/E|''P/E'']], and two ''CCR'' for intact cells, [[L/E|''L/E'']] and [[R/E control ratio|''R/E'']]. | |description='''Coupling control ratios''', ''CCR'', are [[flux control ratio]]s, ''FCR'', at a constant mitochondrial [[substrate state]]. In mitochondrial preparations, there are three well-defined coupling states of respiration, ''L'', ''P'', ''E'' ([[LEAK]], [[OXPHOS]], [[ETS]]). In intact cells, state ''P'' cannot be induced, but a [[ROUTINE]] state of respiration, ''R'', can be measured. The reference state, ''J''<sub>ref</sub>, is defined by taking ''J''<sub>ref</sub> as the maximum flux, i.e. flux in the ETS state, ''E'', such that the lower and upper limits of ''CCR'' are defined as 0.0 and 1.0. Then there are two mitochondrial ''CCR'', [[L/E|''L/E'']] and [[P/E|''P/E'']], and two ''CCR'' for intact cells, [[L/E|''L/E'']] and [[R/E control ratio|''R/E'']]. | ||
|info=[[Flux control ratio]] | |info=[[Flux control ratio]] | ||
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{{MitoPedia concepts | |||
|mitopedia concept=Respiratory control ratio | |||
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{{MitoPedia methods | {{MitoPedia methods | ||
|mitopedia method=Respirometry | |mitopedia method=Respirometry | ||
}} | }} | ||
{{MitoPedia topics | {{MitoPedia O2k and high-resolution respirometry}} | ||
{{MitoPedia topics}} | |||
}} | |||
[[Image:EPL-free and excess.jpg|right|400px|thumb|[[Gnaiger_2014_MitoPathways |The Blue Book 2014]]: Fig. 2.4.]] | [[Image:EPL-free and excess.jpg|right|400px|thumb|[[Gnaiger_2014_MitoPathways |The Blue Book 2014]]: Fig. 2.4.]] | ||
== Coupling control ratios related to free capacities == | == Coupling control ratios related to free capacities == | ||
Revision as of 15:08, 8 February 2016
- high-resolution terminology - matching measurements at high-resolution
Coupling-control ratio
Description
Coupling control ratios, CCR, are flux control ratios, FCR, at a constant mitochondrial substrate state. In mitochondrial preparations, there are three well-defined coupling states of respiration, L, P, E (LEAK, OXPHOS, ETS). In intact cells, state P cannot be induced, but a ROUTINE state of respiration, R, can be measured. The reference state, Jref, is defined by taking Jref as the maximum flux, i.e. flux in the ETS state, E, such that the lower and upper limits of CCR are defined as 0.0 and 1.0. Then there are two mitochondrial CCR, L/E and P/E, and two CCR for intact cells, L/E and R/E.
Abbreviation: CCR
Reference: Flux control ratio
MitoPedia concepts:
Respiratory control ratio
MitoPedia methods:
Respirometry
The Blue Book 2014: Fig. 2.4.
- Free OXPHOS capacity, ≈P = P-L
- OXPHOS coupling efficiency, j≈P = ≈P/P = 1-L/P
- Free ROUTINE activity, ≈R = R-L
- ROUTINE coupling efficiency, j≈R = ≈R/R = 1-L/R
- LEAK control ratio, L/E
- Free ETS capacity, ≈E = E-L
- ETS coupling efficiency, j≈E = ≈E/E = 1-L/E
- netOXPHOS control ratio, ≈P/E=(P-L)/E
- Free OXPHOS capacity, ≈P = P-L
- netROUTINE control ratio, ≈R/E=(R-L)/E
- Free ROUTINE activity, ≈R = R-L
- More details: »ETS coupling efficiency
- OXPHOS control ratio, P/E
- Excess E-P capacity, ExP = E-P
- Excess E-P capacity factor, jExP = ExP/E = 1-P/E
- Excess E-R capacity, ExR = E-R
- Excess E-R capacity factor, jExR = ExR/E = 1-R/E
Compare
- » Coupling control factor, CCF = 1-CCR
- » Respiratory acceptor control ratio, RCR
