Difference between revisions of "Coupling-control ratio"

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Coupling-control ratio

Description

Coupling control ratios, CCR, are flux control ratios, FCR, at a constant mitochondrial substrate control state. In mitochondrial preparations, there are three well-defined coupling states of respiration, L, P, E (LEAK, OXPHOS, ETS). In intact cells, state P cannot be induced, but a ROUTINE state of respiration, R, can be measured. The reference state, J_ref, is defined by taking J_ref as the maximum flux, i.e. flux in the ETS state, E, such that the lower and upper limits of the CCR are defined as 0.0 and 1.0. Then there are two mitochondrial CCR, L/E and P/E, and two CCR for intact cells, L/E and R/E.

Abbreviation: CCR

Reference: Flux control ratio

MitoPedia concepts: Respiratory control ratio

MitoPedia methods: Respirometry

The Blue Book 2014: Fig. 2.4.

Coupling control ratios related to free capacities

L/P coupling control ratio, L/P

Free OXPHOS capacity, ≈P = P-L

OXPHOS coupling efficiency, j_≈P = ≈P/P = 1-L/P

L/R coupling control ratio, L/R

Free ROUTINE activity, ≈R = R-L

ROUTINE coupling efficiency, j_≈R = ≈R/R = 1-L/R

LEAK control ratio, L/E

Free ETS capacity, ≈E = E-L

ETS coupling efficiency, j_≈E = ≈E/E = 1-L/E

netOXPHOS control ratio, ≈P/E=(P-L)/E

Free OXPHOS capacity, ≈P = P-L

netROUTINE control ratio, ≈R/E=(R-L)/E

Free ROUTINE activity, ≈R = R-L

More details » ETS coupling efficiency

Coupling control ratios related to excess capacities

OXPHOS control ratio, P/E

Excess E-P capacity, ExP = E-P

Excess E-P capacity factor, j_ExP = ExP/E = 1-P/E

ROUTINE control ratio, R/E

Excess E-R capacity, ExR = E-R

Excess E-R capacity factor, j_ExR = ExR/E = 1-R/E

Compare

» Coupling control factor, CCF = 1-CCR

» Respiratory acceptor control ratio, RCR

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 {{MitoPedia
 |abbr=''CCR''
-|description='''Coupling control ratios''', ''CCR'', are [[flux control ratio]]s, ''FCR'', at a constant mitochondrial [[substrate state]]. In mitochondrial preparations, there are three well-defined coupling states of respiration, ''L'', ''P'', ''E'' ([[LEAK]], [[OXPHOS]], [[ETS]]). In intact cells, state ''P'' cannot be induced, but a [[ROUTINE]] state of respiration, ''R'', can be measured. The reference state, ''J''<sub>ref</sub>, is defined by taking ''J''<sub>ref</sub> as the maximum flux, i.e. flux in the ETS state, ''E'', such that the lower and upper limits of ''CCR'' are defined as 0.0 and 1.0. Then there are two mitochondrial ''CCR'', [[L/E|''L/E'']] and [[P/E|''P/E'']], and two ''CCR'' for intact cells, [[L/E|''L/E'']] and [[R/E control ratio|''R/E'']].
+|description='''Coupling control ratios''', ''CCR'', are [[flux control ratio]]s, ''FCR'', at a constant mitochondrial [[substrate control state]]. In mitochondrial preparations, there are three well-defined coupling states of respiration, ''L'', ''P'', ''E'' ([[LEAK]], [[OXPHOS]], [[ETS]]). In intact cells, state ''P'' cannot be induced, but a [[ROUTINE]] state of respiration, ''R'', can be measured. The reference state, ''J''<sub>ref</sub>, is defined by taking ''J''<sub>ref</sub> as the maximum flux, i.e. flux in the ETS state, ''E'', such that the lower and upper limits of the ''CCR'' are defined as 0.0 and 1.0. Then there are two mitochondrial ''CCR'', [[L/E|''L/E'']] and [[P/E|''P/E'']], and two ''CCR'' for intact cells, [[L/E|''L/E'']] and [[R/E control ratio|''R/E'']].
 |info=[[Flux control ratio]]
 }}
@@ Line 14: / Line 14: @@
 [[Image:EPL-free and excess.jpg|right|400px|thumb|[[Gnaiger_2014_MitoPathways |The Blue Book 2014]]: Fig. 2.4.]]
 == Coupling control ratios related to free capacities ==
-* [[L/P coupling control ratio |''L/P'' coupling control ratio]], ''L/P''
+:::* [[L/P coupling control ratio |''L/P'' coupling control ratio]], ''L/P''
-:: [[Free OXPHOS capacity]], ''≈P = P-L''
+:::: [[Free OXPHOS capacity]], ''≈P = P-L''
-:: [[OXPHOS coupling efficiency]], ''j<sub>≈P</sub>'' = ''≈P''/''P'' = 1-''L/P''
+:::: [[OXPHOS coupling efficiency]], ''j<sub>≈P</sub>'' = ''≈P''/''P'' = 1-''L/P''
-* [[L/R coupling control ratio |''L/R'' coupling control ratio]], ''L/R''
+:::* [[L/R coupling control ratio |''L/R'' coupling control ratio]], ''L/R''
-:: [[Free ROUTINE activity]], ''≈R = R-L''
+:::: [[Free ROUTINE activity]], ''≈R = R-L''
-:: [[ROUTINE coupling efficiency]], ''j<sub>≈R</sub>'' = ''≈R''/''R'' = 1-''L/R''
+:::: [[ROUTINE coupling efficiency]], ''j<sub>≈R</sub>'' = ''≈R''/''R'' = 1-''L/R''
-* [[LEAK control ratio]], ''L/E''
+:::* [[LEAK control ratio]], ''L/E''
-:: [[Free ETS capacity]], ''≈E = E-L''
+:::: [[Free ETS capacity]], ''≈E = E-L''
-:: [[ETS coupling efficiency]], ''j<sub>≈E</sub>'' = ''≈E''/''E'' = 1-''L/E''
+:::: [[ETS coupling efficiency]], ''j<sub>≈E</sub>'' = ''≈E''/''E'' = 1-''L/E''
-* [[netOXPHOS control ratio]], ''≈P/E''=(''P-L'')/''E''
+:::* [[netOXPHOS control ratio]], ''≈P/E''=(''P-L'')/''E''
-:: [[Free OXPHOS capacity]], ''≈P = P-L''
+:::: [[Free OXPHOS capacity]], ''≈P = P-L''
-* [[netROUTINE control ratio]], ''≈R/E''=(''R-L'')/''E''
+:::* [[netROUTINE control ratio]], ''≈R/E''=(''R-L'')/''E''
-:: [[Free ROUTINE activity]], ''≈R = R-L''
+:::: [[Free ROUTINE activity]], ''≈R = R-L''
-* ''More details:'' »[[ETS coupling efficiency]]
+:::* ''More details'' » [[ETS coupling efficiency]]
 == Coupling control ratios related to excess capacities ==
-* [[OXPHOS control ratio]], ''P/E''
+:::* [[OXPHOS control ratio]], ''P/E''
-:: [[Excess E-P capacity |Excess ''E-P'' capacity]], ''ExP = E-P''
+:::: [[Excess E-P capacity |Excess ''E-P'' capacity]], ''ExP = E-P''
-:: [[Excess E-P capacity factor |Excess ''E-P'' capacity factor]], ''j<sub>ExP</sub>'' = ''ExP''/''E'' = 1-''P/E''
+:::: [[Excess E-P capacity factor |Excess ''E-P'' capacity factor]], ''j<sub>ExP</sub>'' = ''ExP''/''E'' = 1-''P/E''
-* [[ROUTINE control ratio]], ''R/E''
+:::* [[ROUTINE control ratio]], ''R/E''
-:: [[Excess E-R capacity |Excess ''E-R'' capacity]], ''ExR = E-R''
+:::: [[Excess E-R capacity |Excess ''E-R'' capacity]], ''ExR = E-R''
-:: [[Excess E-R capacity factor |Excess ''E-R'' capacity factor]], ''j<sub>ExR</sub>'' = ''ExR''/''E'' = 1-''R/E''
+:::: [[Excess E-R capacity factor |Excess ''E-R'' capacity factor]], ''j<sub>ExR</sub>'' = ''ExR''/''E'' = 1-''R/E''