Difference between revisions of "Coupling-control ratio"
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|description='''Coupling control ratios''', ''CCR'', are [[flux control ratio]]s, ''FCR'', at a constant mitochondrial [[substrate state]]. In mitochondrial preparations, there are three well-defined coupling states of respiration, ''L'', ''P'', ''E'' ([[LEAK]], [[OXPHOS]], [[ETS]]). In intact cells, state ''P'' cannot be induced, but a [[ROUTINE]] state of respiration, ''R'', can be measured. The reference state, ''J''<sub>ref</sub>, is defined by taking ''J''<sub>ref</sub> as the maximum flux, i.e. flux in the ETS state, ''E'', such that the lower and upper limits of ''CCR'' are defined as 0.0 and 1.0. Then there are two mitochondrial ''CCR'', [[L/E|''L/E'']] and [[P/E|''P/E'']], and two ''CCR'' for intact cells, [[L/E|''L/E'']] and [[R/E|''R/E'']]. | |description='''Coupling control ratios''', ''CCR'', are [[flux control ratio]]s, ''FCR'', at a constant mitochondrial [[substrate state]]. In mitochondrial preparations, there are three well-defined coupling states of respiration, ''L'', ''P'', ''E'' ([[LEAK]], [[OXPHOS]], [[ETS]]). In intact cells, state ''P'' cannot be induced, but a [[ROUTINE]] state of respiration, ''R'', can be measured. The reference state, ''J''<sub>ref</sub>, is defined by taking ''J''<sub>ref</sub> as the maximum flux, i.e. flux in the ETS state, ''E'', such that the lower and upper limits of ''CCR'' are defined as 0.0 and 1.0. Then there are two mitochondrial ''CCR'', [[L/E|''L/E'']] and [[P/E|''P/E'']], and two ''CCR'' for intact cells, [[L/E|''L/E'']] and [[R/E|''R/E'']]. | ||
|info=[[Gnaiger_2009_IJBCB]], [[Pesta_2011_Protocols]] | |info=[[Gnaiger_2009_IJBCB]], [[Pesta_2011_Protocols]] | ||
}} | |||
{{MitoPedia methods | |||
|mitopedia method=Respirometry | |||
}} | }} | ||
{{MitoPedia topics | {{MitoPedia topics | ||
Revision as of 19:48, 15 November 2011
- high-resolution terminology - matching measurements at high-resolution
Coupling-control ratio
Description
Coupling control ratios, CCR, are flux control ratios, FCR, at a constant mitochondrial substrate state. In mitochondrial preparations, there are three well-defined coupling states of respiration, L, P, E (LEAK, OXPHOS, ETS). In intact cells, state P cannot be induced, but a ROUTINE state of respiration, R, can be measured. The reference state, Jref, is defined by taking Jref as the maximum flux, i.e. flux in the ETS state, E, such that the lower and upper limits of CCR are defined as 0.0 and 1.0. Then there are two mitochondrial CCR, L/E and P/E, and two CCR for intact cells, L/E and R/E.
Abbreviation: CCR
Reference: Gnaiger_2009_IJBCB, Pesta_2011_Protocols
MitoPedia methods:
Respirometry
MitoPedia topics: "Respiratory state" is not in the list (Enzyme, Medium, Inhibitor, Substrate and metabolite, Uncoupler, Sample preparation, Permeabilization agent, EAGLE, MitoGlobal Organizations, MitoGlobal Centres, ...) of allowed values for the "MitoPedia topic" property.
Respiratory state"Respiratory state" is not in the list (Enzyme, Medium, Inhibitor, Substrate and metabolite, Uncoupler, Sample preparation, Permeabilization agent, EAGLE, MitoGlobal Organizations, MitoGlobal Centres, ...) of allowed values for the "MitoPedia topic" property.
