Difference between revisions of "Difference spectrum"
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Latest revision as of 14:38, 8 February 2016
- high-resolution terminology - matching measurements at high-resolution
Difference spectrum
Description
A difference spectrum is an absorbance spectrum obtained by subtracting that of one substance from that of another. For example, a difference spectrum may be plotted of the absorbance spectrum obtain ed from reduced cytochrome c and subtracting the absorbance spectrum from the same concentration of cytochrome c in its oxidised state. The difference spectrum may be used to quantify the amount to which the cytochrome c is reduced. This can be achieved with the aid of a reference spectrum (or spectra) and the least squares method.
MitoPedia methods: Spectrophotometry
