Difference between revisions of "Dihydroethidium"
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|abbr=DHE | |abbr=DHE | ||
|description='''Dihydroethidium''' (also called hydroethidine) is a cell permeant fluorescent probe used to analyse superoxide presence. It is a reduced form of ethidium that presents blue fluorescence, and after oxidation by superoxide becomes able to intercalate DNA and emits red fluorescence (excitation wavelength ~518β535 nm, emission ~605β610 nm). | |description='''Dihydroethidium''' (also called hydroethidine) is a cell permeant fluorescent probe used to analyse superoxide presence. It is a reduced form of ethidium that presents blue fluorescence, and after oxidation by superoxide becomes able to intercalate DNA and emits red fluorescence (excitation wavelength ~518β535 nm, emission ~605β610 nm). It has been used to detect superoxide by HPLC and by fluorescence microscopy. | ||
|info=[https://www.ncbi.nlm.nih.gov/pubmed/18554538 Laurindo 2008 Methods Enzymol], [https://www.ncbi.nlm.nih.gov/pubmed/12523938 Kalivendi 2003 Biochem J] | |info=[https://www.ncbi.nlm.nih.gov/pubmed/18554538 Laurindo 2008 Methods Enzymol], [https://www.ncbi.nlm.nih.gov/pubmed/12523938 Kalivendi 2003 Biochem J] | ||
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Latest revision as of 13:46, 3 August 2020
Description
Dihydroethidium (also called hydroethidine) is a cell permeant fluorescent probe used to analyse superoxide presence. It is a reduced form of ethidium that presents blue fluorescence, and after oxidation by superoxide becomes able to intercalate DNA and emits red fluorescence (excitation wavelength ~518β535 nm, emission ~605β610 nm). It has been used to detect superoxide by HPLC and by fluorescence microscopy.
Abbreviation: DHE
Reference: Laurindo 2008 Methods Enzymol, Kalivendi 2003 Biochem J