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Doerrier 2016b Abstract Mito Xmas Meeting Innsbruck - Revision history
2024-03-29T15:50:09Z
Revision history for this page on the wiki
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https://wiki.oroboros.at/index.php?title=Doerrier_2016b_Abstract_Mito_Xmas_Meeting_Innsbruck&diff=223705&oldid=prev
Gnaiger Erich at 16:41, 10 January 2022
2022-01-10T16:41:12Z
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<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 16:41, 10 January 2022</td>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>[[OXPHOS]] and [[Electron transfer pathway]] respiratory capacities of isolated mitochondria (normalized per mg mt-protein) were decreased after 1-h WI of the excised heart. A significant injury of the outer mt-membrane is consistent with ischemia-induced mt-permeability transition [3], which can explain a general respiratory defect. In addition, application of a newly developed substrate-uncoupler-inhibitor titration (SUIT) protocol [4] revealed a specific defect of fatty acid β-oxidation (FAO) [5] H<sub>2</sub>O<sub>2</sub> flux based on the Amplex red assay more than doubled after application of AF and DNCB inhibitors to the controls. The glutathione and thioredoxin antioxidant system did not protect mitochondria after WI from this increased H<sub>2</sub>O<sub>2</sub> production. Taken together, standardized respiratory SUIT protocols combined with SOPs in the fluorometric assay of H<sub>2</sub>O<sub>2</sub> production offer a sensitive diagnostic tool for comprehensive OXPHOS analysis.</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>[[OXPHOS]] and [[Electron transfer pathway]] respiratory capacities of isolated mitochondria (normalized per mg mt-protein) were decreased after 1-h WI of the excised heart. A significant injury of the outer mt-membrane is consistent with ischemia-induced mt-permeability transition [3], which can explain a general respiratory defect. In addition, application of a newly developed substrate-uncoupler-inhibitor titration (SUIT) protocol [4] revealed a specific defect of fatty acid β-oxidation (FAO) [5] H<sub>2</sub>O<sub>2</sub> flux based on the Amplex red assay more than doubled after application of AF and DNCB inhibitors to the controls. The glutathione and thioredoxin antioxidant system did not protect mitochondria after WI from this increased H<sub>2</sub>O<sub>2</sub> production. Taken together, standardized respiratory SUIT protocols combined with SOPs in the fluorometric assay of H<sub>2</sub>O<sub>2</sub> production offer a sensitive diagnostic tool for comprehensive OXPHOS analysis.</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>|mipnetlab=AT Innsbruck Oroboros <del style="font-weight: bold; text-decoration: none;">, AT Innsbruck Gnaiger E</del>, AT Innsbruck MitoFit</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>|mipnetlab=AT Innsbruck Oroboros, AT Innsbruck MitoFit</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>}}</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>}}</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Affiliations ==</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Affiliations ==</div></td></tr>
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Gnaiger Erich
https://wiki.oroboros.at/index.php?title=Doerrier_2016b_Abstract_Mito_Xmas_Meeting_Innsbruck&diff=202711&oldid=prev
Beno Marija at 07:44, 3 June 2020
2020-06-03T07:44:55Z
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>In the present work we used high-resolution respirometry (O2k-Fluorometer; Oroboros , Innsbruck, Austria) [1] to investigate simultaneously respiration and hydrogen peroxide production (H<sub>2</sub>O<sub>2</sub>) of mitochondria isolated from the hearts of C57BL/6 mice. By using inhibitors of the main mitochondrial H<sub>2</sub>O<sub>2</sub> scavengers (DNCB for glutathione and AF for thioredoxin) we evaluated the total H<sub>2</sub>O<sub>2</sub> production compared to net H<sub>2</sub>O<sub>2</sub> production in the absence of these inhibitors [2]. </div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>In the present work we used high-resolution respirometry (O2k-Fluorometer; Oroboros , Innsbruck, Austria) [1] to investigate simultaneously respiration and hydrogen peroxide production (H<sub>2</sub>O<sub>2</sub>) of mitochondria isolated from the hearts of C57BL/6 mice. By using inhibitors of the main mitochondrial H<sub>2</sub>O<sub>2</sub> scavengers (DNCB for glutathione and AF for thioredoxin) we evaluated the total H<sub>2</sub>O<sub>2</sub> production compared to net H<sub>2</sub>O<sub>2</sub> production in the absence of these inhibitors [2]. </div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>[[OXPHOS]] and [[<del style="font-weight: bold; text-decoration: none;">ET-</del>pathway]] respiratory capacities of isolated mitochondria (normalized per mg mt-protein) were decreased after 1-h WI of the excised heart. A significant injury of the outer mt-membrane is consistent with ischemia-induced mt-permeability transition [3], which can explain a general respiratory defect. In addition, application of a newly developed substrate-uncoupler-inhibitor titration (SUIT) protocol [4] revealed a specific defect of fatty acid β-oxidation (FAO) [5] H<sub>2</sub>O<sub>2</sub> flux based on the Amplex red assay more than doubled after application of AF and DNCB inhibitors to the controls. The glutathione and thioredoxin antioxidant system did not protect mitochondria after WI from this increased H<sub>2</sub>O<sub>2</sub> production. Taken together, standardized respiratory SUIT protocols combined with SOPs in the fluorometric assay of H<sub>2</sub>O<sub>2</sub> production offer a sensitive diagnostic tool for comprehensive OXPHOS analysis.</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>[[OXPHOS]] and [[<ins style="font-weight: bold; text-decoration: none;">Electron transfer </ins>pathway]] respiratory capacities of isolated mitochondria (normalized per mg mt-protein) were decreased after 1-h WI of the excised heart. A significant injury of the outer mt-membrane is consistent with ischemia-induced mt-permeability transition [3], which can explain a general respiratory defect. In addition, application of a newly developed substrate-uncoupler-inhibitor titration (SUIT) protocol [4] revealed a specific defect of fatty acid β-oxidation (FAO) [5] H<sub>2</sub>O<sub>2</sub> flux based on the Amplex red assay more than doubled after application of AF and DNCB inhibitors to the controls. The glutathione and thioredoxin antioxidant system did not protect mitochondria after WI from this increased H<sub>2</sub>O<sub>2</sub> production. Taken together, standardized respiratory SUIT protocols combined with SOPs in the fluorometric assay of H<sub>2</sub>O<sub>2</sub> production offer a sensitive diagnostic tool for comprehensive OXPHOS analysis.</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|mipnetlab=AT Innsbruck Oroboros , AT Innsbruck Gnaiger E, AT Innsbruck MitoFit</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|mipnetlab=AT Innsbruck Oroboros , AT Innsbruck Gnaiger E, AT Innsbruck MitoFit</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>}}</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>}}</div></td></tr>
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Beno Marija
https://wiki.oroboros.at/index.php?title=Doerrier_2016b_Abstract_Mito_Xmas_Meeting_Innsbruck&diff=195931&oldid=prev
Gnaiger Erich at 11:50, 7 March 2020
2020-03-07T11:50:42Z
<p></p>
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<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 11:50, 7 March 2020</td>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>[[OXPHOS]] and [[ET-pathway]] respiratory capacities of isolated mitochondria (normalized per mg mt-protein) were decreased after 1-h WI of the excised heart. A significant injury of the outer mt-membrane is consistent with ischemia-induced mt-permeability transition [3], which can explain a general respiratory defect. In addition, application of a newly developed substrate-uncoupler-inhibitor titration (SUIT) protocol [4] revealed a specific defect of fatty acid β-oxidation (FAO) [5] H<sub>2</sub>O<sub>2</sub> flux based on the Amplex red assay more than doubled after application of AF and DNCB inhibitors to the controls. The glutathione and thioredoxin antioxidant system did not protect mitochondria after WI from this increased H<sub>2</sub>O<sub>2</sub> production. Taken together, standardized respiratory SUIT protocols combined with SOPs in the fluorometric assay of H<sub>2</sub>O<sub>2</sub> production offer a sensitive diagnostic tool for comprehensive OXPHOS analysis.</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>[[OXPHOS]] and [[ET-pathway]] respiratory capacities of isolated mitochondria (normalized per mg mt-protein) were decreased after 1-h WI of the excised heart. A significant injury of the outer mt-membrane is consistent with ischemia-induced mt-permeability transition [3], which can explain a general respiratory defect. In addition, application of a newly developed substrate-uncoupler-inhibitor titration (SUIT) protocol [4] revealed a specific defect of fatty acid β-oxidation (FAO) [5] H<sub>2</sub>O<sub>2</sub> flux based on the Amplex red assay more than doubled after application of AF and DNCB inhibitors to the controls. The glutathione and thioredoxin antioxidant system did not protect mitochondria after WI from this increased H<sub>2</sub>O<sub>2</sub> production. Taken together, standardized respiratory SUIT protocols combined with SOPs in the fluorometric assay of H<sub>2</sub>O<sub>2</sub> production offer a sensitive diagnostic tool for comprehensive OXPHOS analysis.</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|mipnetlab=AT Innsbruck Oroboros , AT Innsbruck Gnaiger E, AT Innsbruck MitoFit</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|mipnetlab=AT Innsbruck Oroboros , AT Innsbruck Gnaiger E, AT Innsbruck MitoFit</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">}}</del></div></td><td colspan="2"></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">{{Labeling</del></div></td><td colspan="2"></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">|area=Respiration</del></div></td><td colspan="2"></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">|injuries=Ischemia-reperfusion</del></div></td><td colspan="2"></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">|organism=Mouse</del></div></td><td colspan="2"></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">|tissues=Heart</del></div></td><td colspan="2"></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">|preparations=Isolated mitochondria</del></div></td><td colspan="2"></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">|couplingstates=LEAK, OXPHOS, ET</del></div></td><td colspan="2"></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">|pathways=F, N, S, Gp, CIV, ROX</del></div></td><td colspan="2"></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">|instruments=Oxygraph-2k, O2k-Fluorometer</del></div></td><td colspan="2"></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">|event=B2, Oral</del></div></td><td colspan="2"></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">|additional=Warm ischemia, Amplex UltraRed, RP1, RP2, AF, DNCB</del></div></td><td colspan="2"></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>}}</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>}}</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Affiliations ==</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Affiliations ==</div></td></tr>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::::# Doerrier C, Sumbalova Z, Krumschnabel G, Hiller E, Gnaiger E (2016) SUIT reference protocol for OXPHOS analysis by high-resolution respirometry. 21.06 Mitochondr Physiol Network.</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::::# Doerrier C, Sumbalova Z, Krumschnabel G, Hiller E, Gnaiger E (2016) SUIT reference protocol for OXPHOS analysis by high-resolution respirometry. 21.06 Mitochondr Physiol Network.</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::::# Lemieux H, Semsroth S, Antretter H, Höfer D, Gnaiger E (2011) Mitochondrial respiratory control and early defects of oxidative phosphorylation in the failing human heart. Int J Biochem Cell Biol 43:1729–38.</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::::# Lemieux H, Semsroth S, Antretter H, Höfer D, Gnaiger E (2011) Mitochondrial respiratory control and early defects of oxidative phosphorylation in the failing human heart. Int J Biochem Cell Biol 43:1729–38.</div></td></tr>
<tr><td colspan="2"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">{{Labeling</ins></div></td></tr>
<tr><td colspan="2"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">|area=Respiration</ins></div></td></tr>
<tr><td colspan="2"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">|injuries=Ischemia-reperfusion</ins></div></td></tr>
<tr><td colspan="2"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">|organism=Mouse</ins></div></td></tr>
<tr><td colspan="2"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">|tissues=Heart</ins></div></td></tr>
<tr><td colspan="2"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">|preparations=Isolated mitochondria</ins></div></td></tr>
<tr><td colspan="2"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">|couplingstates=LEAK, OXPHOS, ET</ins></div></td></tr>
<tr><td colspan="2"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">|pathways=F, N, S, Gp, CIV, ROX</ins></div></td></tr>
<tr><td colspan="2"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">|instruments=Oxygraph-2k, O2k-Fluorometer</ins></div></td></tr>
<tr><td colspan="2"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">|event=B2, Oral</ins></div></td></tr>
<tr><td colspan="2"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">|additional=Warm ischemia, AmR, RP1, RP2, AF, DNCB</ins></div></td></tr>
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</table>
Gnaiger Erich
https://wiki.oroboros.at/index.php?title=Doerrier_2016b_Abstract_Mito_Xmas_Meeting_Innsbruck&diff=171331&oldid=prev
Beno Marija at 13:47, 23 January 2019
2019-01-23T13:47:44Z
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<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 13:47, 23 January 2019</td>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|event=Mito Xmas Meeting 2016 Innsbruck AT</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|event=Mito Xmas Meeting 2016 Innsbruck AT</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|abstract=Ischemia-reperfusion damage during the transplant process occurs mainly in three steps: (i) warm ischemia (WI), (ii) cold ischemia, (iii) reperfusion. WI is defined as the time interval between a tissue remaining at body temperature after blood supply has been reduced or interrupted. Oxidative stress is considered to be one of the main causes of injury during ischemia-reperfusion.</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|abstract=Ischemia-reperfusion damage during the transplant process occurs mainly in three steps: (i) warm ischemia (WI), (ii) cold ischemia, (iii) reperfusion. WI is defined as the time interval between a tissue remaining at body temperature after blood supply has been reduced or interrupted. Oxidative stress is considered to be one of the main causes of injury during ischemia-reperfusion.</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>In the present work we used high-resolution respirometry (O2k-Fluorometer; <del style="font-weight: bold; text-decoration: none;">OROBOROS</del>, Innsbruck, Austria) [1] to investigate simultaneously respiration and hydrogen peroxide production (H<sub>2</sub>O<sub>2</sub>) of mitochondria isolated from the hearts of C57BL/6 mice. By using inhibitors of the main mitochondrial H<sub>2</sub>O<sub>2</sub> scavengers (DNCB for glutathione and AF for thioredoxin) we evaluated the total H<sub>2</sub>O<sub>2</sub> production compared to net H<sub>2</sub>O<sub>2</sub> production in the absence of these inhibitors [2]. </div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>In the present work we used high-resolution respirometry (O2k-Fluorometer; <ins style="font-weight: bold; text-decoration: none;">Oroboros </ins>, Innsbruck, Austria) [1] to investigate simultaneously respiration and hydrogen peroxide production (H<sub>2</sub>O<sub>2</sub>) of mitochondria isolated from the hearts of C57BL/6 mice. By using inhibitors of the main mitochondrial H<sub>2</sub>O<sub>2</sub> scavengers (DNCB for glutathione and AF for thioredoxin) we evaluated the total H<sub>2</sub>O<sub>2</sub> production compared to net H<sub>2</sub>O<sub>2</sub> production in the absence of these inhibitors [2]. </div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>[[OXPHOS]] and [[ET-pathway]] respiratory capacities of isolated mitochondria (normalized per mg mt-protein) were decreased after 1-h WI of the excised heart. A significant injury of the outer mt-membrane is consistent with ischemia-induced mt-permeability transition [3], which can explain a general respiratory defect. In addition, application of a newly developed substrate-uncoupler-inhibitor titration (SUIT) protocol [4] revealed a specific defect of fatty acid β-oxidation (FAO) [5] H<sub>2</sub>O<sub>2</sub> flux based on the Amplex red assay more than doubled after application of AF and DNCB inhibitors to the controls. The glutathione and thioredoxin antioxidant system did not protect mitochondria after WI from this increased H<sub>2</sub>O<sub>2</sub> production. Taken together, standardized respiratory SUIT protocols combined with SOPs in the fluorometric assay of H<sub>2</sub>O<sub>2</sub> production offer a sensitive diagnostic tool for comprehensive OXPHOS analysis.</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>[[OXPHOS]] and [[ET-pathway]] respiratory capacities of isolated mitochondria (normalized per mg mt-protein) were decreased after 1-h WI of the excised heart. A significant injury of the outer mt-membrane is consistent with ischemia-induced mt-permeability transition [3], which can explain a general respiratory defect. In addition, application of a newly developed substrate-uncoupler-inhibitor titration (SUIT) protocol [4] revealed a specific defect of fatty acid β-oxidation (FAO) [5] H<sub>2</sub>O<sub>2</sub> flux based on the Amplex red assay more than doubled after application of AF and DNCB inhibitors to the controls. The glutathione and thioredoxin antioxidant system did not protect mitochondria after WI from this increased H<sub>2</sub>O<sub>2</sub> production. Taken together, standardized respiratory SUIT protocols combined with SOPs in the fluorometric assay of H<sub>2</sub>O<sub>2</sub> production offer a sensitive diagnostic tool for comprehensive OXPHOS analysis.</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>|mipnetlab=AT Innsbruck <del style="font-weight: bold; text-decoration: none;">OROBOROS</del>, AT Innsbruck Gnaiger E, AT Innsbruck MitoFit</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>|mipnetlab=AT Innsbruck <ins style="font-weight: bold; text-decoration: none;">Oroboros </ins>, AT Innsbruck Gnaiger E, AT Innsbruck MitoFit</div></td></tr>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>{{Labeling</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>{{Labeling</div></td></tr>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>:::: Doerrier C(1), Gnaiger E(1,2)</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>:::: Doerrier C(1), Gnaiger E(1,2)</div></td></tr>
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<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>::::# <del style="font-weight: bold; text-decoration: none;">OROBOROS INSTRUMENTS</del>, Innsbruck, Austria </div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>::::# <ins style="font-weight: bold; text-decoration: none;">Oroboros Instruments</ins>, Innsbruck, Austria </div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::::# D. Swarovski Research Lab, Dept Visceral, Transplant Thoracic Surgery, Medical Univ Innsbruck, Austria</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::::# D. Swarovski Research Lab, Dept Visceral, Transplant Thoracic Surgery, Medical Univ Innsbruck, Austria</div></td></tr>
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</table>
Beno Marija
https://wiki.oroboros.at/index.php?title=Doerrier_2016b_Abstract_Mito_Xmas_Meeting_Innsbruck&diff=145192&oldid=prev
Beno Marija at 15:48, 9 November 2017
2017-11-09T15:48:40Z
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<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>|couplingstates=LEAK, OXPHOS, <del style="font-weight: bold; text-decoration: none;">ETS</del></div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>|couplingstates=LEAK, OXPHOS, <ins style="font-weight: bold; text-decoration: none;">ET</ins></div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|pathways=F, N, S, Gp, CIV, ROX</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|pathways=F, N, S, Gp, CIV, ROX</div></td></tr>
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</table>
Beno Marija
https://wiki.oroboros.at/index.php?title=Doerrier_2016b_Abstract_Mito_Xmas_Meeting_Innsbruck&diff=144298&oldid=prev
Beno Marija at 12:20, 20 October 2017
2017-10-20T12:20:05Z
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>In the present work we used high-resolution respirometry (O2k-Fluorometer; OROBOROS, Innsbruck, Austria) [1] to investigate simultaneously respiration and hydrogen peroxide production (H<sub>2</sub>O<sub>2</sub>) of mitochondria isolated from the hearts of C57BL/6 mice. By using inhibitors of the main mitochondrial H<sub>2</sub>O<sub>2</sub> scavengers (DNCB for glutathione and AF for thioredoxin) we evaluated the total H<sub>2</sub>O<sub>2</sub> production compared to net H<sub>2</sub>O<sub>2</sub> production in the absence of these inhibitors [2]. </div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>In the present work we used high-resolution respirometry (O2k-Fluorometer; OROBOROS, Innsbruck, Austria) [1] to investigate simultaneously respiration and hydrogen peroxide production (H<sub>2</sub>O<sub>2</sub>) of mitochondria isolated from the hearts of C57BL/6 mice. By using inhibitors of the main mitochondrial H<sub>2</sub>O<sub>2</sub> scavengers (DNCB for glutathione and AF for thioredoxin) we evaluated the total H<sub>2</sub>O<sub>2</sub> production compared to net H<sub>2</sub>O<sub>2</sub> production in the absence of these inhibitors [2]. </div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>[[OXPHOS]] and [[<del style="font-weight: bold; text-decoration: none;">ETS</del>]] respiratory capacities of isolated mitochondria (normalized per mg mt-protein) were decreased after 1-h WI of the excised heart. A significant injury of the outer mt-membrane is consistent with ischemia-induced mt-permeability transition [3], which can explain a general respiratory defect. In addition, application of a newly developed substrate-uncoupler-inhibitor titration (SUIT) protocol [4] revealed a specific defect of fatty acid β-oxidation (FAO) [5] H<sub>2</sub>O<sub>2</sub> flux based on the Amplex red assay more than doubled after application of AF and DNCB inhibitors to the controls. The glutathione and thioredoxin antioxidant system did not protect mitochondria after WI from this increased H<sub>2</sub>O<sub>2</sub> production. Taken together, standardized respiratory SUIT protocols combined with SOPs in the fluorometric assay of H<sub>2</sub>O<sub>2</sub> production offer a sensitive diagnostic tool for comprehensive OXPHOS analysis.</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>[[OXPHOS]] and [[<ins style="font-weight: bold; text-decoration: none;">ET-pathway</ins>]] respiratory capacities of isolated mitochondria (normalized per mg mt-protein) were decreased after 1-h WI of the excised heart. A significant injury of the outer mt-membrane is consistent with ischemia-induced mt-permeability transition [3], which can explain a general respiratory defect. In addition, application of a newly developed substrate-uncoupler-inhibitor titration (SUIT) protocol [4] revealed a specific defect of fatty acid β-oxidation (FAO) [5] H<sub>2</sub>O<sub>2</sub> flux based on the Amplex red assay more than doubled after application of AF and DNCB inhibitors to the controls. The glutathione and thioredoxin antioxidant system did not protect mitochondria after WI from this increased H<sub>2</sub>O<sub>2</sub> production. Taken together, standardized respiratory SUIT protocols combined with SOPs in the fluorometric assay of H<sub>2</sub>O<sub>2</sub> production offer a sensitive diagnostic tool for comprehensive OXPHOS analysis.</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|mipnetlab=AT Innsbruck OROBOROS, AT Innsbruck Gnaiger E, AT Innsbruck MitoFit</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|mipnetlab=AT Innsbruck OROBOROS, AT Innsbruck Gnaiger E, AT Innsbruck MitoFit</div></td></tr>
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</table>
Beno Marija
https://wiki.oroboros.at/index.php?title=Doerrier_2016b_Abstract_Mito_Xmas_Meeting_Innsbruck&diff=128344&oldid=prev
Doerrier Carolina at 16:17, 14 December 2016
2016-12-14T16:17:07Z
<p></p>
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<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 16:17, 14 December 2016</td>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|tissues=Heart</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|tissues=Heart</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|preparations=Isolated mitochondria</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|preparations=Isolated mitochondria</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>|couplingstates=OXPHOS, ETS</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>|couplingstates=<ins style="font-weight: bold; text-decoration: none;">LEAK, </ins>OXPHOS, ETS</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>|pathways=F</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>|pathways=F<ins style="font-weight: bold; text-decoration: none;">, N, S, Gp, CIV, ROX</ins></div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|instruments=Oxygraph-2k, O2k-Fluorometer</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|instruments=Oxygraph-2k, O2k-Fluorometer</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|event=B2, Oral</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|event=B2, Oral</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>|additional=Amplex <del style="font-weight: bold; text-decoration: none;">Red</del></div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>|additional=<ins style="font-weight: bold; text-decoration: none;">Warm ischemia, </ins>Amplex <ins style="font-weight: bold; text-decoration: none;">UltraRed, RP1, RP2, AF, DNCB</ins></div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>}}</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>}}</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Affiliations ==</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Affiliations ==</div></td></tr>
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Doerrier Carolina
https://wiki.oroboros.at/index.php?title=Doerrier_2016b_Abstract_Mito_Xmas_Meeting_Innsbruck&diff=128226&oldid=prev
Gnaiger Erich at 15:44, 13 December 2016
2016-12-13T15:44:31Z
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<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>|event=<del style="font-weight: bold; text-decoration: none;">Poster</del></div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>|event=<ins style="font-weight: bold; text-decoration: none;">B2, Oral</ins></div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|additional=Amplex Red</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|additional=Amplex Red</div></td></tr>
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</table>
Gnaiger Erich
https://wiki.oroboros.at/index.php?title=Doerrier_2016b_Abstract_Mito_Xmas_Meeting_Innsbruck&diff=128198&oldid=prev
Kandolf Georg at 10:16, 13 December 2016
2016-12-13T10:16:20Z
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>{{Abstract</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>{{Abstract</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>|title=Searching for specific targets of ischemic damage of cardiac mitochondria using O2k-Fluorometry.</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>|title=Searching for specific targets of ischemic damage of cardiac mitochondria using <ins style="font-weight: bold; text-decoration: none;">[[</ins>O2k-Fluorometry<ins style="font-weight: bold; text-decoration: none;">]]</ins>.</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|authors=Doerrier C, Gnaiger E</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|authors=Doerrier C, Gnaiger E</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|year=2016</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|year=2016</div></td></tr>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|abstract=Ischemia-reperfusion damage during the transplant process occurs mainly in three steps: (i) warm ischemia (WI), (ii) cold ischemia, (iii) reperfusion. WI is defined as the time interval between a tissue remaining at body temperature after blood supply has been reduced or interrupted. Oxidative stress is considered to be one of the main causes of injury during ischemia-reperfusion.</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|abstract=Ischemia-reperfusion damage during the transplant process occurs mainly in three steps: (i) warm ischemia (WI), (ii) cold ischemia, (iii) reperfusion. WI is defined as the time interval between a tissue remaining at body temperature after blood supply has been reduced or interrupted. Oxidative stress is considered to be one of the main causes of injury during ischemia-reperfusion.</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>In the present work we used high-resolution respirometry (O2k-Fluorometer; OROBOROS, Innsbruck, Austria) [1] to investigate simultaneously respiration and hydrogen peroxide production (H<sub>2</sub>O<sub>2</sub>) of mitochondria isolated from the hearts of C57BL/6 mice. By using inhibitors of the main mitochondrial H<sub>2</sub>O<sub>2</sub> scavengers (DNCB for glutathione and AF for thioredoxin) we evaluated the total H<sub>2</sub>O<sub>2</sub> production compared to net H<sub>2</sub>O<sub>2</sub> production in the absence of these inhibitors [2]. </div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>In the present work we used high-resolution respirometry (O2k-Fluorometer; OROBOROS, Innsbruck, Austria) [1] to investigate simultaneously respiration and hydrogen peroxide production (H<sub>2</sub>O<sub>2</sub>) of mitochondria isolated from the hearts of C57BL/6 mice. By using inhibitors of the main mitochondrial H<sub>2</sub>O<sub>2</sub> scavengers (DNCB for glutathione and AF for thioredoxin) we evaluated the total H<sub>2</sub>O<sub>2</sub> production compared to net H<sub>2</sub>O<sub>2</sub> production in the absence of these inhibitors [2]. </div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>OXPHOS and ETS respiratory capacities of isolated mitochondria (normalized per mg mt-protein) were decreased after 1-h WI of the excised heart. A significant injury of the outer mt-membrane is consistent with ischemia-induced mt-permeability transition [3], which can explain a general respiratory defect. In addition, application of a newly developed substrate-uncoupler-inhibitor titration (SUIT) protocol [4] revealed a specific defect of fatty acid β-oxidation (FAO) [5] <del style="font-weight: bold; text-decoration: none;">H2O2 </del>flux based on the Amplex red assay more than doubled after application of AF and DNCB inhibitors to the controls. The glutathione and thioredoxin antioxidant system did not protect mitochondria after WI from this increased <del style="font-weight: bold; text-decoration: none;">H2O2 </del>production. Taken together, standardized respiratory SUIT protocols combined with SOPs in the fluorometric assay of <del style="font-weight: bold; text-decoration: none;">H2O2 </del>production offer a sensitive diagnostic tool for comprehensive OXPHOS analysis.</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div> </div></td></tr>
<tr><td colspan="2"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">[[</ins>OXPHOS<ins style="font-weight: bold; text-decoration: none;">]] </ins>and <ins style="font-weight: bold; text-decoration: none;">[[</ins>ETS<ins style="font-weight: bold; text-decoration: none;">]] </ins>respiratory capacities of isolated mitochondria (normalized per mg mt-protein) were decreased after 1-h WI of the excised heart. A significant injury of the outer mt-membrane is consistent with ischemia-induced mt-permeability transition [3], which can explain a general respiratory defect. In addition, application of a newly developed substrate-uncoupler-inhibitor titration (SUIT) protocol [4] revealed a specific defect of fatty acid β-oxidation (FAO) [5] <ins style="font-weight: bold; text-decoration: none;">H<sub>2</sub>O<sub>2</sub> </ins>flux based on the Amplex red assay more than doubled after application of AF and DNCB inhibitors to the controls. The glutathione and thioredoxin antioxidant system did not protect mitochondria after WI from this increased <ins style="font-weight: bold; text-decoration: none;">H<sub>2</sub>O<sub>2</sub> </ins>production. Taken together, standardized respiratory SUIT protocols combined with SOPs in the fluorometric assay of <ins style="font-weight: bold; text-decoration: none;">H<sub>2</sub>O<sub>2</sub> </ins>production offer a sensitive diagnostic tool for comprehensive OXPHOS analysis.</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|mipnetlab=AT Innsbruck OROBOROS, AT Innsbruck Gnaiger E, AT Innsbruck MitoFit</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|mipnetlab=AT Innsbruck OROBOROS, AT Innsbruck Gnaiger E, AT Innsbruck MitoFit</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>}}</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>}}</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>{{Labeling</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>{{Labeling</div></td></tr>
<tr><td colspan="2"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">|area=Respiration</ins></div></td></tr>
<tr><td colspan="2"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">|injuries=Ischemia-reperfusion</ins></div></td></tr>
<tr><td colspan="2"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">|organism=Mouse</ins></div></td></tr>
<tr><td colspan="2"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">|tissues=Heart</ins></div></td></tr>
<tr><td colspan="2"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">|preparations=Isolated mitochondria</ins></div></td></tr>
<tr><td colspan="2"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">|couplingstates=OXPHOS, ETS</ins></div></td></tr>
<tr><td colspan="2"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">|pathways=F</ins></div></td></tr>
<tr><td colspan="2"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">|instruments=Oxygraph-2k, O2k-Fluorometer</ins></div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|event=Poster</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|event=Poster</div></td></tr>
<tr><td colspan="2"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">|additional=Amplex Red</ins></div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>}}</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>}}</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Affiliations ==</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Affiliations ==</div></td></tr>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::::# OROBOROS INSTRUMENTS, Innsbruck, Austria </div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::::# OROBOROS INSTRUMENTS, Innsbruck, Austria </div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>::::# D.Swarovski Research Lab, Dept<del style="font-weight: bold; text-decoration: none;">.</del>Visceral, Transplant Thoracic Surgery, Medical Univ Innsbruck, Austria<del style="font-weight: bold; text-decoration: none;">.</del></div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>::::# D. Swarovski Research Lab, Dept Visceral, Transplant Thoracic Surgery, Medical Univ Innsbruck, Austria</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>==Reference==</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>==Reference==</div></td></tr>
</table>
Kandolf Georg
https://wiki.oroboros.at/index.php?title=Doerrier_2016b_Abstract_Mito_Xmas_Meeting_Innsbruck&diff=128197&oldid=prev
Kandolf Georg at 09:36, 13 December 2016
2016-12-13T09:36:14Z
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<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 09:36, 13 December 2016</td>
</tr><tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l5">Line 5:</td>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|event=Mito Xmas Meeting 2016 Innsbruck AT</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|event=Mito Xmas Meeting 2016 Innsbruck AT</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|abstract=Ischemia-reperfusion damage during the transplant process occurs mainly in three steps: (i) warm ischemia (WI), (ii) cold ischemia, (iii) reperfusion. WI is defined as the time interval between a tissue remaining at body temperature after blood supply has been reduced or interrupted. Oxidative stress is considered to be one of the main causes of injury during ischemia-reperfusion.</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|abstract=Ischemia-reperfusion damage during the transplant process occurs mainly in three steps: (i) warm ischemia (WI), (ii) cold ischemia, (iii) reperfusion. WI is defined as the time interval between a tissue remaining at body temperature after blood supply has been reduced or interrupted. Oxidative stress is considered to be one of the main causes of injury during ischemia-reperfusion.</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>In the present work we used high-resolution respirometry (O2k-Fluorometer; OROBOROS, Innsbruck, Austria) [1] to investigate simultaneously respiration and hydrogen peroxide production (<del style="font-weight: bold; text-decoration: none;">H2O2</del>) of mitochondria isolated from the hearts of C57BL/6 mice. By using inhibitors of the main mitochondrial <del style="font-weight: bold; text-decoration: none;">H2O2 </del>scavengers (DNCB for glutathione and AF for thioredoxin) we evaluated the total <del style="font-weight: bold; text-decoration: none;">H2O2 </del>production compared to net <del style="font-weight: bold; text-decoration: none;">H2O2 </del>production in the absence of these inhibitors [2]. </div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>In the present work we used high-resolution respirometry (O2k-Fluorometer; OROBOROS, Innsbruck, Austria) [1] to investigate simultaneously respiration and hydrogen peroxide production (<ins style="font-weight: bold; text-decoration: none;">H<sub>2</sub>O<sub>2</sub></ins>) of mitochondria isolated from the hearts of C57BL/6 mice. By using inhibitors of the main mitochondrial <ins style="font-weight: bold; text-decoration: none;">H<sub>2</sub>O<sub>2</sub> </ins>scavengers (DNCB for glutathione and AF for thioredoxin) we evaluated the total <ins style="font-weight: bold; text-decoration: none;">H<sub>2</sub>O<sub>2</sub> </ins>production compared to net <ins style="font-weight: bold; text-decoration: none;">H<sub>2</sub>O<sub>2</sub> </ins>production in the absence of these inhibitors [2]. </div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>OXPHOS and ETS respiratory capacities of isolated mitochondria (normalized per mg mt-protein) were decreased after 1-h WI of the excised heart. A significant injury of the outer mt-membrane is consistent with ischemia-induced mt-permeability transition [3], which can explain a general respiratory defect. In addition, application of a newly developed substrate-uncoupler-inhibitor titration (SUIT) protocol [4] revealed a specific defect of fatty acid β-oxidation (FAO) [5] H2O2 flux based on the Amplex red assay more than doubled after application of AF and DNCB inhibitors to the controls. The glutathione and thioredoxin antioxidant system did not protect mitochondria after WI from this increased H2O2 production. Taken together, standardized respiratory SUIT protocols combined with SOPs in the fluorometric assay of H2O2 production offer a sensitive diagnostic tool for comprehensive OXPHOS analysis.</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>OXPHOS and ETS respiratory capacities of isolated mitochondria (normalized per mg mt-protein) were decreased after 1-h WI of the excised heart. A significant injury of the outer mt-membrane is consistent with ischemia-induced mt-permeability transition [3], which can explain a general respiratory defect. In addition, application of a newly developed substrate-uncoupler-inhibitor titration (SUIT) protocol [4] revealed a specific defect of fatty acid β-oxidation (FAO) [5] H2O2 flux based on the Amplex red assay more than doubled after application of AF and DNCB inhibitors to the controls. The glutathione and thioredoxin antioxidant system did not protect mitochondria after WI from this increased H2O2 production. Taken together, standardized respiratory SUIT protocols combined with SOPs in the fluorometric assay of H2O2 production offer a sensitive diagnostic tool for comprehensive OXPHOS analysis.</div></td></tr>
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Kandolf Georg