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Doerrier 2016b Abstract Mito Xmas Meeting Innsbruck

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Searching for specific targets of ischemic damage of cardiac mitochondria using O2k-Fluorometry.

Link:

Doerrier C, Gnaiger E (2016)

Event: Mito Xmas Meeting 2016 Innsbruck AT

Ischemia-reperfusion damage during the transplant process occurs mainly in three steps: (i) warm ischemia (WI), (ii) cold ischemia, (iii) reperfusion. WI is defined as the time interval between a tissue remaining at body temperature after blood supply has been reduced or interrupted. Oxidative stress is considered to be one of the main causes of injury during ischemia-reperfusion. In the present work we used high-resolution respirometry (O2k-Fluorometer; OROBOROS, Innsbruck, Austria) [1] to investigate simultaneously respiration and hydrogen peroxide production (H2O2) of mitochondria isolated from the hearts of C57BL/6 mice. By using inhibitors of the main mitochondrial H2O2 scavengers (DNCB for glutathione and AF for thioredoxin) we evaluated the total H2O2 production compared to net H2O2 production in the absence of these inhibitors [2].

OXPHOS and ETS respiratory capacities of isolated mitochondria (normalized per mg mt-protein) were decreased after 1-h WI of the excised heart. A significant injury of the outer mt-membrane is consistent with ischemia-induced mt-permeability transition [3], which can explain a general respiratory defect. In addition, application of a newly developed substrate-uncoupler-inhibitor titration (SUIT) protocol [4] revealed a specific defect of fatty acid β-oxidation (FAO) [5] H2O2 flux based on the Amplex red assay more than doubled after application of AF and DNCB inhibitors to the controls. The glutathione and thioredoxin antioxidant system did not protect mitochondria after WI from this increased H2O2 production. Taken together, standardized respiratory SUIT protocols combined with SOPs in the fluorometric assay of H2O2 production offer a sensitive diagnostic tool for comprehensive OXPHOS analysis.


O2k-Network Lab: AT Innsbruck OROBOROS, AT Innsbruck Gnaiger E, AT Innsbruck MitoFit


Labels: MiParea: Respiration 

Stress:Ischemia-reperfusion  Organism: Mouse  Tissue;cell: Heart  Preparation: Isolated mitochondria 


Coupling state: OXPHOS, ETS"ETS" is not in the list (LEAK, ROUTINE, OXPHOS, ET) of allowed values for the "Coupling states" property.  Pathway:HRR: Oxygraph-2k, O2k-Fluorometer  Event: B2, Oral  Amplex Red 

Affiliations

Doerrier C(1), Gnaiger E(1,2)
  1. OROBOROS INSTRUMENTS, Innsbruck, Austria
  2. D. Swarovski Research Lab, Dept Visceral, Transplant Thoracic Surgery, Medical Univ Innsbruck, Austria

Reference

  1. Makrecka-Kuka M, Krumschnabel G, Gnaiger E (2015) High-resolution respirometry for simultaneous measurement of oxygen and hydrogen peroxide fluxes in permeabilized cells, tissue homogenate and isolated mitochondria. Biomolecules 5:1319-38.
  2. Aon MA, Stanley AS, Sivakumaran V, Kembro JM, O´Rourke B, Paolocci N, Cortassa S (2012) Glutathione/thioredoxin sytems modulate mitochondrial H2O2 emission: An experimental-computational study. J Gen Physiol 139(6):479-91.
  3. Borutaite V, Toleikis A, Brown GC (2013) In the eye of the storm: mitochondrial damage during heart and brain ischaemia. FEBS J 280:4999-5014.
  4. Doerrier C, Sumbalova Z, Krumschnabel G, Hiller E, Gnaiger E (2016) SUIT reference protocol for OXPHOS analysis by high-resolution respirometry. 21.06 Mitochondr Physiol Network.
  5. Lemieux H, Semsroth S, Antretter H, Höfer D, Gnaiger E (2011) Mitochondrial respiratory control and early defects of oxidative phosphorylation in the failing human heart. Int J Biochem Cell Biol 43:1729–38.