Doerrier C

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MiPsociety
COST Action CA15203 MitoEAGLE
Evolution-Age-Gender-Lifestyle-Environment: mitochondrial fitness mapping


 

Doerrier C


MitoPedia topics: EAGLE 

COST: Member COST WG1: WG1 COST WG2: WG2 COST WG3: WG3 COST WG4: WG4

COST ECI: ECI

MC Substitute - Management Committee MitoEAGLE


Name Doerrier Velasco Carolina, PhD.
Institution
Carolina Doerrier

Oroboros Instruments

Mitochondria and Cell Research

Chief scientific officer


Background: ES Granada Acuna-Castroviejo D

Carolina Doerrier joined Oroboros in October 2014.

Address Schoepfstr. 18, 6020
City Innsbruck
State/Province
Country Austria
Email carolina.doerrier@oroboros.at
Weblink www.oroboros.at, www.bioblast.at, www.mitophysiology.org
O2k-Network Lab AT Innsbruck Oroboros, ES Granada Acuna-Castroviejo D


Labels: Field of research: Basic  Topics: High-Resolution FluoRespirometry, Mitochondria, Melatonin, Reactive oxygen species, Reactive nitrogen species, Sepsis 


Publications

 PublishedReference
Gnaiger 2019 MitoFit Preprint Arch2019Gnaiger E, Aasander Frostner E, Abdul Karim N, Abdel-Rahman EA, Abumrad NA, Acuna-Castroviejo D, Adiele RC, et al (2019) Mitochondrial respiratory states and rates. MitoFit Preprint Arch doi:10.26124/mitofit:190001.v6.
Doerrier 2019 MitoFit Preprint Arch EA2019Doerrier C, Gama-Perez P, Distefano G, Pesta D, Soendergaard SD, Chroeis KM, Gonzalez-Franquesa A, Goodpaster BH, Coen P, Larsen S, Gnaiger E, Garcia-Roves PM (2019) Inter-laboratory harmonization of respiratory protocols in permeabilized human muscle fibers. MitoFit Preprint Arch doi:10.26124/mitofit:ea19.MiPSchool.0009.
Komlodi 2018 Methods Mol Biol2018Komlodi T, Sobotka O, Krumschnabel G, Bezuidenhout N, Hiller E, Doerrier C, Gnaiger E (2018) Comparison of mitochondrial incubation media for measurement of respiration and hydrogen peroxide production. Methods Mol Biol 1782:137-55.
Ost 2018 Curr Opin Clin Nutr Metab Care2018Ost M, Doerrier C, Gama-Perez P, Moreno-Gomez S (2018) Analysis of mitochondrial respiratory function in tissue biopsies and blood cells. Curr Opin Clin Nutr Metab Care 21:336-42.
Doerrier 2018 Methods Mol Biol2018Doerrier C, Garcia-Souza LF, Krumschnabel G, Wohlfarter Y, Mészáros AT, Gnaiger E (2018) High-Resolution FluoRespirometry and OXPHOS protocols for human cells, permeabilized fibers from small biopsies of muscle, and isolated mitochondria. Methods Mol Biol 1782:31-70.
Bernardinelli 2017 PLoS One2017Bernardinelli E, Costa R, Scantamburlo G, To J, Morabito R, Nofziger C, Doerrier C, Krumschnabel G, Paulmichl M, Dossena S (2017) Mis-targeting of the mitochondrial protein LIPT2 leads to apoptotic cell death. PLoS One 12(6):e0179591.
Volani 2017 Metallomics2017Volani C, Doerrier C, Demetz E, Haschka D, Paglia G, Lavdas AA, Gnaiger E, Weiss G (2017) Dietary iron loading negatively affects liver mitochondrial function. Metallomics 9:1634-44.
Lopez 2017 PLOS ONE2017López A, Ortiz F, Doerrier C, Venegas C, Fernández-Ortiz M, Aranda P, Díaz-Casado ME, Fernández-Gil B, Barriocanal-Casado E, Escames G, López L, Acuña-Castroviejo D (2017) Mitochondrial impairment and melatonin protection in parkinsonian mice do not depend of inducible or neuronal nitric oxide synthases. PLOS ONE 12:e0183090.
Doerrier 2016 Mitochondrion2016Doerrier C, García JA, Volt H, Díaz-Casado ME, Luna-Sánchez M, Fernández-Gil B, Escames G, López LC, Acuña-Castroviejo D (2016) Permeabilized myocardial fibers as model to detect mitochondrial dysfunction during sepsis and melatonin effects without disruption of mitochondrial network. Mitochondrion 27:56-63.
Volt 2016 J Pineal Res2016Volt H, García JA, Doerrier C, Díaz-Casado ME, Guerra-Librero A, López LC, Escames G, Tresguerres JA, Acuña-Castroviejo D (2016) Same molecule but different expression: aging and sepsis trigger NLRP3 inflammasome activation, a target of melatonin. J Pineal Res 60:193-205.
Doerrier 2015 Thesis2015Doerrier C (2015) Estudio bioenergetica mitochondrial en la sepsis experimental y efecto protector de la Melatonina. Doctoral Thesis p154.
Ortiz 2015 J Pineal Res2015Ortiz F, Acuña-Castroviejo D, Doerrier C, Dayoub JC, López LC, Venegas C, García JA, López A, Volt H, Luna-Sánchez M, Escames G (2015) Melatonin blunts the mitochondrial/NLRP3 connection and protects against radiation-induced oral mucositis. J Pineal Res 58:34-49.
Melatonin2015Doerrier C (2015) Melatonin and attenuation of mitochondrial oxidative damage. Mitochondr Physiol Network 2015-03-03.
Garcia 2015 FASEB J2015García JA, Volt H, Venegas C, Doerrier C, Escames G, López LC, Acuña-Castroviejo D (2015) Disruption of the NF-κB/NLRP3 connection by melatonin requires retinoid-related orphan receptor-α and blocks the septic response in mice. FASEB J 29:3863-75.
García-Corzo 2015 Biochim Biophys Acta2015García-Corzo L, Luna-Sánchez M, Doerrier C, Ortiz F, Escames G, Acuña-Castroviejo D, López LC (2015) Ubiquinol-10 ameliorates mitochondrial encephalopathy associated with CoQ deficiency. Biochim Biophys Acta 1842:893-901.
Ortiz 2014 J Pineal Res2014Ortiz F, García JA, Acuña-Castroviejo D, Doerrier C, López A, Venegas C, Volt H, Luna-Sánchez M, López LC, Escames G (2014) The beneficial effects of melatonin against heart mitochondrial impairment during sepsis: inhibition of iNOS and preservation of nNOS. J Pineal Res 56:71-81.
Doerrier 2014 Life Sci2014Doerrier C, García JA, Volt H, Díaz-Casado ME, Lima-Cabello E, Ortiz F, Luna-Sánchez M, Escames G, López LC, Acuña-Castroviejo D (2014) Identification of mitochondrial deficits and melatonin targets in liver of septic mice by high-resolution respirometry. Life Sci 121:158-65.
Concepcion-Huertas 2013 J Sports Sci2013Concepcion-Huertas M, Chirosa LJ, De Haro T, Chirosa IJ, Romero V, Aguilar-Martinez D, Leonardo-Mendonça RC, Doerrier C, Escames G, Acuña-Castroviejo D (2013) Changes in the redox status and inflammatory response in handball players during one-year of competition and training. J Sports Sci 31:1197-207.
Escames 2013 Horm Mol Biol Clin Investig2013Escames G, Diaz-Casado ME, Doerrier C, Luna-Sanchez M, Lopez LC, Acuna-Castroviejo D (2013) Early gender differences in the redox status of the brain mitochondria with age: effects of melatonin therapy. Horm Mol Biol Clin Investig 16(2):91-100.
Garcia-Corzo 2013 Hum Mol Genet2013García-Corzo L, Luna-Sánchez M, Doerrier C, García JA, Guarás A, Acín-Pérez R, Bullejos-Peregrín J, López A, Escames G, Enríquez JA, Acuña-Castroviejo D, López LC (2013) Dysfunctional Coq9 protein causes predominant encephalomyopathy associated with CoQ deficiency. Hum Mol Genet 22:1233-48.
Acuña-Castroviejo 2012 Age (Dordr)2012Acuña-Castroviejo D, Carretero M, Doerrier C, López LC, García-Corzo L, Tresguerres JA, Escames G (2012) Melatonin protects lung mitochondria from aging. Age (Dordr) 34(3):681-92.

Abstracts

 PublishedReference
Ravasz 2019 Abstract IOC1412019Ravasz D, Bui D, Kitayev A, Greenwood B, Hill C, Komlodi T, Doerrier C, Ozohanics O, Moore AL, Gnaiger E, Kiebish M, Kolev K, Seyfried TN, Willis WT, Narain N, Adam-Vizi V, Chinopoulos C (2019) Endogenous quinones sustain a moderate NADH oxidation by complex I during anoxia. Mitochondr Physiol Network 24.02.
Gnaiger 2019 NatureConference2019Gnaiger E, Passrugger M, Gallee L, Felipe F Garcia-Souza, Zdrazilova L, Doerrier C (2019) High-resolution respirometry and innovations in the Oroboros O2k-technology.
Silva 2019 ESCI20192019Silva FSG, Komlodi T, Garcia-Souza LF, Bento G, Doerrier C, Oliveira PJ, Gnaiger E (2019) Can fatty acid oxidation be specifically blocked by the CPT1 inhibitor etomoxir?
Garcia-Roves 2019 MiPschool Coimbra2019
Pablo Garcia-Roves
Generating reference values on mitochondrial respiration in permeabilized muscle fibers.
Silva 2019 MiPschool Coimbra2019
Filomena Pereira da Silva Grilo da Silva
Off-target effects of etomoxir on mitochondrial complex I.
Doerrier 2018 MiP2018a2018
Carolina Anneliese Doerrier
Inter-laboratory harmonization of protocols for mitochondrial function evaluation in permeabilized muscle fibers.
Doerrier 2018 ESCI20182018
Carolina Anneliese Doerrier
High-resolution respirometry, two techniques combined in one to explore mitochondrial function in health and disease
Garcia-Souza 2018 Life Sciences Meeting 2018 Innsbruck AT2018A respirometric cell viability test for peripheral-blood mononuclear cells and platelets.
Doerrier 2018 MiP2018c2018
Carolina Doerrier
Inter-laboratory study on human permeabilized myofibers: effects of fiber preparation, respiration buffer, and blebbistatin on mitochondrial respiratory capacity and oxygen limitation.
Doerrier 2018 EBEC20182018
Carolina Anneliese Doerrier
Respiratory mapping of mitochondrial pathways for establishing a database of mitochondrial physiology.
Garcia-Roves 2018 MiP2018b2018
Pablo Garcia-Roves
Generating mitochondrial respirometry reference values from permeabilized mouse soleus muscle fibers. Working Group 2 report. Garcia-Roves_Presentation
Komlodi 2018b EBEC20182018Endogenous quinones sustain NADH oxidation by Complex I during anoxia, supporting substrate-level phosphorylation in mouse liver mitochondria.
Ravasz 2018 Abstract The evolving concept of mitochondria2018Vast pools of endogenous quinones sustain NADH oxidation by Complex I during anoxia, supporting substrate-level phosphorylation in mouse liver mitochondria
Makrecka-Kuka 2018 MiP20182018
Marina Makrecka-Kuka
Fatty acid oxidation in brain: from aging to ischemia and sepsis.
Gnaiger 2018 AussieMit2018Komlodi T, Sobotka O, Doerrier C, Gnaiger E (2018) Mitochondrial H2O2 production is low under tissue normoxia but high at in-vitro air-level oxygen pressure - comparison of LEAK and OXPHOS states. AussieMit 2018 Melbourne AU.
Sobotka 2018 MiP20182018
Ondrej Sobotka
Measurement of ROS production under hypoxia and unexpected methodological pitfalls of Amplex UltraRed assay.
Doerrier 2018 MiP2018b2018
Carolina Doerrier
Evaluation of anaplerotic pathways to avoid artefacts in respirometric measurement of fatty acid oxidation.
Doerrier 2017 MiPschool C22017
DoerrierC.JPG
Substrate-uncoupler-inhibitor titration (SUIT) protocols – fundamental principles.
Komlodi 2017 Abstract MITOEAGLE Barcelona2017
COST Action MitoEAGLE
Succinate dehydrogenase regulation via oxaloacetate in brain mitochondria.
Garcia-Roves 2017 MiP20172017
Pablo Garcia-Roves
Mitochondrial respirometry reference values from permeabilized mouse soleus muscle fibers. MitoEAGLE WG2 pilot study. Garcia-Roves_Presentation
Doerrier 2017 Abstract MitoEAGLE Barcelona2017
COST Action MitoEAGLE
SUIT reference protocol for comparative mitochondrial physiology.
Coen 2017 MiP2017 WG22017
Paul Coen
Human skeletal muscle mitochondrial function: A proposal for a state-of-the-art literature review.
Laner 2017 Abstract EUROMIT2017 Cologne2017
EUROMIT2017
K-Regio MitoFit and MitoEAGLE – towards a global data bank on mitochondrial physiology.
Doerrier 2017 MiP2017 WG22017
Carolina Anneliese Doerrier
Upper limit of OXPHOS capacity in permeabilized myofibers: effect of oxygen, ADP and blebbistatin in MiR05Cr and Buffer Z.
Sobotka 2017 MiPschool Obergurgl2017
Ondrej Sobotka
Reference protocols for ROS production measurements.
Doerrier 2017 MiP2017 C12017
Carolina Anneliese Doerrier
Effects of warm ischemia on cardiac mitochondria to detect specific targets by high-resolution respirometry (HRR)
Liebscher 2017 MiPschool Obergurgl2017
Gudrun Liebscher
The LAMTOR complex in regulation of fat metabolism.
Volani 2016 Abstract Mito Xmas Meeting Innsbruck2016Effects of systemic iron perturbations on mitochondrial activity and on cellular metabolism in vivo.
Doerrier 2016 Abstract MitoFit Science Camp 20162016Development of a SUIT reference protocol for OXPHOS analysis by high-resolution respirometry.
Volani 2016 Abstract MitoFit Science Camp 20162016Effects of iron imbalances on mitochondrial activity in mouse liver homogenate and permabilized rat PBMCs.
Sumbalova 2016a Abstract MitoFit Science Camp 20162016Optimizing strategies on the malate concentration in SUIT protocols.
Gnaiger 2016 Abstract EBEC Riva del Garda 20162016MitoFit and MitoEAGLE – towards a global data bank on mitochondrial physiology.
Gnaiger 2016 Abstract SFRR Australasia 20162016Facts and artefacts in measurements of H2O2 production under hypoxia.
Bezuidenhout 2016a Abstract MitoFit Science Camp 20162016Comparison of oxygen dependence of respiration in permeabilized mouse skeletal muscle fibers in two respiration media, MiR06Cr and Buffer Z containing Ctl, Cr and Blebbistatin.
Doerrier 2016a Abstract Mito Xmas Meeting Innsbruck2016
MitoFit
K-Regio MitoFit and MitoEAGLE – towards a global data bank on mitochondrial physiology.
Bezuidenhout 2016b Abstract MitoFit Science Camp 20162016Analysis and comparison of mitochondrial function using cooling plate or ice during mechanical separation of mouse skeletal muscle fibers.
Doerrier 2016b Abstract Mito Xmas Meeting Innsbruck2016Searching for specific targets of ischemic damage of cardiac mitochondria using O2k-Fluorometry.
Krumschnabel 2016a Abstract MitoFit Science Camp 20162016O2k-Protocols: Mitochondrial preparations for HRR.
Doerrier 2015 Abstract MiPschool Greenville 20152015"Succinate paradox" study by high resolution respirometry combined with fluorometry and spectrofluorometry: NextGen-O2k.
Gnaiger 2015 Abstract MiP20152015Mitochondrial function of cyropreserved HEK 293T cells: development of a reference sample for high-resolution respirometry.
Doerrier 2015 Abstract MiP20152015Simultaneous measurement of mitochondrial respiration, hydrogen peroxide production, and NADH autofluorescence to assess mitochondrial function.
Volani 2015 Abstract IOC1062015Volani C, Haschka D, Demetz E, Doerrier C, Gnaiger E, Weiss G (2015) Determination of mitochondrial respiration in peripheral blood mononuclear cells. Mitochondr Physiol Network 20.10.
Luna-Sánchez 2014 Abstract SECF2014Mitochondrial encephalopathy due to coenzyme q deficiency: pathogenesis and treatment.
Volt 2014 Abstract SECF2014Experimental basis for the “inflammaging” theory.
Garcia 2014 Abstract SECF2014Melatonin disrupts the NF-κB/NLRP3 connection in sepsis through RORα receptor.
Diaz-Casado 2014 Abstract SECF2014Melatonin restores mitochondrial regulatory gene expression and function in the zebrafish model of parkinsonism.
Volt 2014 Abstract The 2014 Ageing Summit2014Effects of melatonin on the age-dependent dysregulation of the Nf-Kb/Nlrp3 activation during sepsis.
Doerrier 2014 Abstract SECF2014Evaluation of melatonin treatment in mitochondria of permeabilized myocardial fibers from septic mice.
Ortiz 2013 Abstract SEOR2013Molecular Basis of the radiotherapy-induced mucositis, beneficial effects of melatonin.
Luna-Sánchez 2012 Abstract MiPsummer School Cambridge2012CoQ deficiency: molecular and phathophysiological consequences.
Luna-Sánchez 2012 Abstract FEPS&SECF2012CoQ deficiency: Molecular and pathophysiological consequences.
Escames 2012 Abstract ESTRO2012Beneficial effects of topical melatonin against mitochondrial damage during radiotherapy-induced mucositis.
Doerrier 2012 Abstract Bioblast2012Doerrier C, Garcia JA, Escames G, Acuna-Castroviejo D (2012) Treatment with melatonin prevents myocardial mitochondrial dysfunction in experimental sepsis in mice. Mitochondr Physiol Network 17.12.
Garcia-Corzo 2011 Abstract Euromit 82011Generation of a mouse model of Coenzyme Q deficiency.
Escames 2011 Abstract FASEB Summer Research Conference2011Melatonin regulates the proliferation and differentation of the mice subventricular neural stem cells. Role of mitochondria.


Oroboros-team

Bioblast Editorial Board

MitoEAGLE Short-Term Scientific Mission

Work Plan summary
WORKPLAN SUMMARY Different experimental aspects (e.g. purity of the preparations, environmental humidity which could affect the wet weight of the muscle, chemicals) will be evaluated during the STSM in order to clarify the sources of variability found in the MitoEAGLE WG2 pilot study. The preparation of the samples will be performed according the SOP established for the MitoEAGLE WG2 pilot study. Permeabilized fibers from soleus of C57BL6/J mice will be used. Mitochondrial respiration will be assessed by O2k High-Resolution FluoRespirometry (HRFR). The Substrate- Uncoupler-Inhibitor Titration (SUIT) protocol used in the MitoEAGLE WG2 pilot study for obtaining mitochondrial respirometry reference values from permeabilized mouse soleus muscle fibers, will be used to perform the experiments during the STSM at the Department of Physiological Sciences II in the Faculty of Medicine (Barcelona University, Barcelona, Spain).


Participated at