Difference between revisions of "Drahota 2005 Cell Biochem Biophys"

Latest revision as of 11:09, 13 February 2015

Drahota Z, Milerova M, Stieglerova A, Skarka L, Houstek J, Ostadal B (2005) Development of cytochrome-c oxidase in rat heart. Cell Biochem Biophys 43:87-94.

» PMID: 16043886

Drahota Z, Milerova M, Stieglerova A, Skarka L, Houstek J, Ostadal B (2005) Cell Biochem Biophys

Abstract: Cytochrome c oxidase (COX) activity of the rat heart was two- to sevenfold activated when the membrane integrity was disrupted by hypotonic swelling, freezing-thawing, or a detergent, indicating that a large portion of COX capacity in intact mitochondria is not active. The effect of detergent was tested in heart mitochondria isolated from 1-, 5-, 15-, 29-, and 60-d-old rats; activation by detergent was up to 20-fold in 1-d-old animals, whereas in mitochondria from 60-d-old rats it was only 7-fold. Our data indicate that the rat heart exhibits significant developmental changes dependent on downregulation of COX activity in the neonatal period. • Keywords: Rat heart, Postnatal development, Cytochrome-c oxidase, Mitochondria, Detergent

• O2k-Network Lab: CZ Prague Houstek J

Labels: MiParea: Respiration

Organism: Rat Tissue;cell: Heart Preparation: Isolated mitochondria, Oxidase;biochemical oxidation Enzyme: Complex IV;cytochrome c oxidase

Coupling state: OXPHOS

HRR: Oxygraph-2k

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 {{Publication
-|title=Drahota Z, Milerova M, Stieglerova A, Skarka L, Houstek J, Ostadal B (2005) Development of cytochrome-c oxidase in rat heart. Cell Biochem. Biophys. 43: 87-94.
+|title=Drahota Z, Milerova M, Stieglerova A, Skarka L, Houstek J, Ostadal B (2005) Development of cytochrome-c oxidase in rat heart. Cell Biochem Biophys 43:87-94.
+|info=[http://www.ncbi.nlm.nih.gov/pubmed/16043886 PMID: 16043886]
 |authors=Drahota Z, Milerova M, Stieglerova A, Skarka L, Houstek J, Ostadal B
 |year=2005
-|journal=Cell. Biochem. Biophys.
+|journal=Cell Biochem Biophys
-|abstract=Cytochrome-c oxidase (COX) activity of the rat heart was two- to sevenfold activated when the membrane integrity was disrupted by hypotonic swelling, freezing-thawing, or a detergent, indicating that a large portion of COX capacity in intact mitochondria is not active. The effect of detergent was tested in heart mitochondria isolated from 1-, 5-, 15-, 29-, and 60-d-old rats; activation by detergent was up to 20-fold in 1-d-old animals, whereas in mitochondria from 60-d-old rats it was only 7-fold. Our data indicate that the rat heart exhibits significant developmental changes dependent on downregulation of COX activity in the neonatal period.
+|abstract=Cytochrome ''c'' oxidase (COX) activity of the rat heart was two- to sevenfold activated when the membrane integrity was disrupted by hypotonic swelling, freezing-thawing, or a detergent, indicating that a large portion of COX capacity in intact mitochondria is not active. The effect of detergent was tested in heart mitochondria isolated from 1-, 5-, 15-, 29-, and 60-d-old rats; activation by detergent was up to 20-fold in 1-d-old animals, whereas in mitochondria from 60-d-old rats it was only 7-fold. Our data indicate that the rat heart exhibits significant developmental changes dependent on downregulation of COX activity in the neonatal period.
 |keywords=Rat heart, Postnatal development, Cytochrome-c oxidase, Mitochondria, Detergent
+|mipnetlab=CZ Prague Houstek J
+|discipline=Biomedicine
 }}
 {{Labeling
-|discipline=Biomedicine
+|area=Respiration
 |organism=Rat
-|preparations=Isolated Mitochondria
+|tissues=Heart
-|enzymes=Complex IV; Cytochrome c Oxidase
+|preparations=Isolated mitochondria, Oxidase;biochemical oxidation
-|topics=Respiration; OXPHOS; ETS Capacity
+|enzymes=Complex IV;cytochrome c oxidase
+|couplingstates=OXPHOS
 |instruments=Oxygraph-2k
-|articletype=Protocol; Manual
+|discipline=Biomedicine
 }}