Du 1999 Free Radic Biol Med
| Du G, Willet K, Mouithys-Mickalad A, Sluse-Goffart CM, Droy-Lefaix M-T, Sluse FE (1999) EGb 761 protects liver mitochondria against injury induced by in vitro anoxia/reoxygenation. Free Radical Biol. Med. 27: 596-604. |
Du G, Willet K, Mouithys-Mickalad A, Sluse-Goffart CM, Droy-Lefaix M-T, Sluse FE (1999) Free Radical Biology & Medicine
Abstract: he present study investigated the protective effects of Ginkgo biloba extract (EGb 761) on rat liver mitochondrial damage induced by in vitro anoxia/reoxygenation. Anoxia/reoxygenation was known to impair respiratory activities and mitochondrial oxidative phosphorylation efficiency. ADP/O (2.57 ยฑ 0.11) decreased after anoxia/reoxygenation (1.75 ยฑ 0.09, p < .01), as well as state 3 and uncoupled respiration (โ20%, p < .01), but state 4 respiration increased (p < .01). EGb 761 (50โ200 ฮผg/ml) had no effect on mitochondrial functions before anoxia, but had a specific dose-dependent protective effect after anoxia/reoxygenation. When mitochondria were incubated with 200 ฮผg/ml EGb 761, they showed an increase in ADP/O (2.09 ยฑ 0.14, p < .05) and a decrease in state 4 respiration (โ22%) after anoxia/reoxygenation. In EPR spin-trapping measurement, EGb 761 decreased the EPR signal of superoxide anion produced during reoxygenation. In conclusion, EGb 761 specially protects mitochondrial ATP synthesis against anoxia/reoxygenation injury by scavenging the superoxide anion generated by mitochondria. โข Keywords: Ginkgo biloba extract, Mitochondria, Oxidative phosphorylation, Anoxia/reoxygenation, Superoxide anion, EPR, Free radicals
Labels:
Organism: Rat, Plant"Plant" is not in the list (Human, Pig, Mouse, Rat, Guinea pig, Bovines, Horse, Dog, Rabbit, Cat, ...) of allowed values for the "Mammal and model" property.
Tissue;cell: Hepatocyte; Liver"Hepatocyte; Liver" is not in the list (Heart, Skeletal muscle, Nervous system, Liver, Kidney, Lung;gill, Islet cell;pancreas;thymus, Endothelial;epithelial;mesothelial cell, Blood cells, Fat, ...) of allowed values for the "Tissue and cell" property.
Preparation: Isolated Mitochondria"Isolated Mitochondria" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property.
Regulation: Respiration; OXPHOS; ETS Capacity"Respiration; OXPHOS; ETS Capacity" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property.
HRR: Oxygraph-2k
