ET capacity: Difference between revisions
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Respiratory electron | Respiratory electron transfer system capacity of mitochondria in the experimentally controlled state of [[non-coupled respiration]] (fully uncoupled; state ''E''), obtained in intact cells or mitochondrial preparations with defined substrates, by titration of an established [[uncoupler]] up to optimum concentration at maximum flux. Non-coupled respiration yields an estimate of ETS capacity. In this state ''[[E]]'', the mt-membrane potential is collapsed, which provides a reference state for flux control ratios and for measurement of the mt-membrane potential. | ||
==References== | ==References== | ||
*Gnaiger E (2009) Capacity of oxidative phosphorylation in human skeletal muscle. New perspectives of mitochondrial physiology. [http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6TCH-4W04KR1-3&_user=10&_rdoc=1&_fmt=&_orig=search&_sort=d&_docanchor=&view=c&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=a6456620daa0d8cca022b5f934f2cf30 | *Gnaiger E (2009) Capacity of oxidative phosphorylation in human skeletal muscle. New perspectives of mitochondrial physiology. Int. J. Biochem. Cell Biol. 41: 1837–1845. [http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6TCH-4W04KR1-3&_user=10&_rdoc=1&_fmt=&_orig=search&_sort=d&_docanchor=&view=c&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=a6456620daa0d8cca022b5f934f2cf30 ''- link''] | ||
*Gnaiger E (2010) MitoPathways: Respiratory states and flux control ratios. [http://www.oroboros.at/index.php?respiratorystates | *Gnaiger E (2010) MitoPathways: Respiratory states and flux control ratios. Mitochondr. Physiol. Network 12.15: 1-5. [http://www.oroboros.at/index.php?respiratorystates ''- link''] | ||
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Revision as of 18:40, 17 August 2010
Respiratory electron transfer system capacity of mitochondria in the experimentally controlled state of non-coupled respiration (fully uncoupled; state E), obtained in intact cells or mitochondrial preparations with defined substrates, by titration of an established uncoupler up to optimum concentration at maximum flux. Non-coupled respiration yields an estimate of ETS capacity. In this state E, the mt-membrane potential is collapsed, which provides a reference state for flux control ratios and for measurement of the mt-membrane potential.
References
- Gnaiger E (2009) Capacity of oxidative phosphorylation in human skeletal muscle. New perspectives of mitochondrial physiology. Int. J. Biochem. Cell Biol. 41: 1837–1845. - link
- Gnaiger E (2010) MitoPathways: Respiratory states and flux control ratios. Mitochondr. Physiol. Network 12.15: 1-5. - link
- high-resolution terminology - matching measurements at high-resolution
ET capacity
