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F-junction

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Revision as of 06:37, 10 September 2016 by Gnaiger Erich (talk | contribs)


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F-junction

Description

F-junction

The F-junction is a junction for convergent electron flow in the electron transfer system (ETS) from fatty acids (F-junction substrates) through fatty acyl CoA dehydrogenase (reduced form FADH2) to electron transferring flavoprotein (CETF), and further transfer through the Q-junction to Complex III (CIII). The concept of the F-junction and N-junction provides a basis for defining categories of SUIT protocols. Fatty acid oxidation (the FAO substrate state) not only depends on electron transfer through the F-junction (which is typically rate-limiting) but simultaneously generates NADH and thus depends on N-junction throughput. Hence FAO can be inhibited completely by inhibition of Complex I (CI). In addition and independent of this source of NADH, the N-junction substrate malate is required as a co-substrate for FAO in mt-preparations, since accumulation of AcetylCoA inhibits FAO in the absence of malate. Malate is oxidized in a reaction catalyzed by malate dehydrogenase to oxaloacetate (yielding NADH), which then stimulates the entry of AcetylCoA into the TCA cycle catalyzed by citrate synthase.


Reference: Gnaiger 2014 MitoPathways


MitoPedia concepts: MiP concept, SUIT concept 


MitoPedia methods: Respirometry 

Contributed by Gnaiger E 2016-02-12