FCCP: Difference between revisions
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== Application in [[HRR]] == | == Application in [[HRR]] == | ||
Revision as of 17:39, 9 March 2011
Description
Carbonyl cyanide p-(trifluoro-methoxy) phenyl-hydrazone (C10H5F3N4O); uncoupler, F(x): FCCP added at concentration x.
Abbreviation: Fx
Reference: MiPNet10.04; SteinlechnerMaran_1996_AJP
MitoPedia topics:
Uncoupler
Application in HRR
F: FCCP (Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone, C10H5F3N4O); Sigma C 2920, 10 mg, store at 4 ยฐC: FW = 254.17)
Caution: Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.
Preparation of 1 mM stock solution (dissolved in 99.9% Ethanol):
- 1) weigh 1.27 mg of FCCP into a glass vial and dissolve in 5 ml Ethanol
- 2) divide into 0.2 ml portions OR store in 5 ml glass vial and use subsamples during experiments to avoid contamination of the stock solution
- 3) store in glass vials (recommendation) at -20 ยฐC
Oxygraph-2k Manual Titrations: MiPNet09.12
- Titration volume: 1 ยตl steps using a 10 ยตl syringe
- Final conc. in 2 ml O2k-chamber: 0.5 ยตM steps
Frequently, more diluted FCCP for getting several titration steps is necessary. In this case prepare more diluted solution (ditution 2-10x) from your stock solution.