Difference between revisions of "Fluorometric dyes"
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{{MitoPedia}} | {{MitoPedia | ||
|description=[[Extrinsic fluorophores]]; fluorescent markers. | |||
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{{MitoPedia concepts}} | |||
{{MitoPedia methods | {{MitoPedia methods | ||
|mitopedia method= | |mitopedia method=Fluorometry | ||
}} | }} | ||
{{MitoPedia O2k and high-resolution respirometry}} | |||
{{MitoPedia topics}} | {{MitoPedia topics}} | ||
== | == Fluorometric dyes for direct quantification == | ||
*ROS ( | *ROS (H<sub>2</sub>O<sub>2</sub>): [[Amplex red]] | ||
*Ca2+ - Dye: Fura2 | *Ca2+ - Dye: [[Fura2]] | ||
*Ca2+ - Dye: Indo1 | *Ca2+ - Dye: [[Indo1]] | ||
*Ca2+ - Dye: Calcium green | *Ca2+ - Dye: Calcium green | ||
*mt-Membrane potential | *mt-Membrane potential: [[Safranin]] | ||
*mt-Membrane potential | *mt-Membrane potential: [[TMRM]] | ||
*mt-Membrane potential, | *mt-Membrane potential, H<sub>2</sub>O<sub>2</sub>: [[Rhodamin 123]] | ||
*pH | *pH: [[Fluorescin]] | ||
*pH | *pH: [[HPTS]] | ||
*Mg2+ | *Mg2+: [[Magnesium organge]], [[Magnesium green]] | ||
*ROS (HOO-.) | *ROS (HOO-.): [[MitoSOX]] | ||
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== | == Fluorometric dyes for ratiometric excitation == | ||
* | *Ca2+: [[Fura2]] | ||
*mt-Membrane potential: [[TMRM]] (?) | |||
*pH: [[SNAFL-1]] | |||
*Redox state: [[roGFP]] | |||
== Fluorometric dyes for ratiometric emmission == | |||
*Ca2+: [[Indo1]] | |||
*mt-Membrane potential: [[JC1]] | |||
== Autofluorescence == | |||
*[[NADH]] | |||
Latest revision as of 14:28, 8 February 2016
Description
Extrinsic fluorophores; fluorescent markers.
MitoPedia methods: Fluorometry
Fluorometric dyes for direct quantification
- ROS (H2O2): Amplex red
- Ca2+ - Dye: Fura2
- Ca2+ - Dye: Indo1
- Ca2+ - Dye: Calcium green
- mt-Membrane potential: Safranin
- mt-Membrane potential: TMRM
- mt-Membrane potential, H2O2: Rhodamin 123
- pH: Fluorescin
- pH: HPTS
- Mg2+: Magnesium organge, Magnesium green
- ROS (HOO-.): MitoSOX