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Garcia-Souza 2017 MiP2017 - Revision history
2024-03-28T12:35:26Z
Revision history for this page on the wiki
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https://wiki.oroboros.at/index.php?title=Garcia-Souza_2017_MiP2017&diff=223719&oldid=prev
Gnaiger Erich at 16:47, 10 January 2022
2022-01-10T16:47:29Z
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<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 16:47, 10 January 2022</td>
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Gnaiger Erich
https://wiki.oroboros.at/index.php?title=Garcia-Souza_2017_MiP2017&diff=223718&oldid=prev
Gnaiger Erich at 16:47, 10 January 2022
2022-01-10T16:47:08Z
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<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 16:47, 10 January 2022</td>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>In summary, PBMC and PLT respiration was not affected during the first 48 h at altitude. Our results suggest that short-term expsure to mild altitude is not a variable which interferes with mitochondrial functional diagnostics in pathological states. However, longer exposure to altitude and the effect of higher altitude exposure should be investigated.</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>In summary, PBMC and PLT respiration was not affected during the first 48 h at altitude. Our results suggest that short-term expsure to mild altitude is not a variable which interferes with mitochondrial functional diagnostics in pathological states. However, longer exposure to altitude and the effect of higher altitude exposure should be investigated.</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|editor=[[Kandolf G]]</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|editor=[[Kandolf G]]</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>|mipnetlab=AT Innsbruck Oroboros, SK Bratislava Sumbalova Z, AT Innsbruck Burtscher M, CH Zurich Lundby C, CH Zurich University of Zurich Physiology, DK Copenhagen Lundby C<del style="font-weight: bold; text-decoration: none;">, AT Innsbruck Gnaiger E</del></div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>|mipnetlab=AT Innsbruck Oroboros, SK Bratislava Sumbalova Z, AT Innsbruck Burtscher M, CH Zurich Lundby C, CH Zurich University of Zurich Physiology, DK Copenhagen Lundby C</div></td></tr>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Affiliations and support ==</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Affiliations and support ==</div></td></tr>
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Gnaiger Erich
https://wiki.oroboros.at/index.php?title=Garcia-Souza_2017_MiP2017&diff=165239&oldid=prev
Gnaiger Erich at 10:17, 3 November 2018
2018-11-03T10:17:42Z
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<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 10:17, 3 November 2018</td>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|mipnetlab=AT Innsbruck Oroboros, SK Bratislava Sumbalova Z, AT Innsbruck Burtscher M, CH Zurich Lundby C, CH Zurich University of Zurich Physiology, DK Copenhagen Lundby C, AT Innsbruck Gnaiger E</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|mipnetlab=AT Innsbruck Oroboros, SK Bratislava Sumbalova Z, AT Innsbruck Burtscher M, CH Zurich Lundby C, CH Zurich University of Zurich Physiology, DK Copenhagen Lundby C, AT Innsbruck Gnaiger E</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">}}</del></div></td><td colspan="2"></td></tr>
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<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">|additional=PMBCs, MitoEAGLE,</del></div></td><td colspan="2"></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>}}</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>}}</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Affiliations and support ==</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Affiliations and support ==</div></td></tr>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::::# Garcia-Souza LF, Cizmarova B, Sumbalova Z, Menz V, Burtscher M, Gnaiger E (2017) Assessment of mitochondrial respiratory function in cryopreserved platelets. Abstract, MiPschool Obergurgl 2017.</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::::# Garcia-Souza LF, Cizmarova B, Sumbalova Z, Menz V, Burtscher M, Gnaiger E (2017) Assessment of mitochondrial respiratory function in cryopreserved platelets. Abstract, MiPschool Obergurgl 2017.</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::::# Sumbalova Z, Hiller E, Chang S, Garcia L, Droescher S, Calabria E, Volani C, Krumschnabel G, Gnaiger E (2016) Isolation of blood cells for HRR. Mitochondr Physiol Network 21.17:1-15.</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::::# Sumbalova Z, Hiller E, Chang S, Garcia L, Droescher S, Calabria E, Volani C, Krumschnabel G, Gnaiger E (2016) Isolation of blood cells for HRR. Mitochondr Physiol Network 21.17:1-15.</div></td></tr>
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<tr><td colspan="2"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">|couplingstates=ROUTINE, ET</ins></div></td></tr>
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Gnaiger Erich
https://wiki.oroboros.at/index.php?title=Garcia-Souza_2017_MiP2017&diff=165238&oldid=prev
Gnaiger Erich at 10:16, 3 November 2018
2018-11-03T10:16:43Z
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<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 10:16, 3 November 2018</td>
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<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>|additional=PMBCs,</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>|additional=PMBCs<ins style="font-weight: bold; text-decoration: none;">, MitoEAGLE</ins>,</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>}}</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>}}</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Affiliations and support ==</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Affiliations and support ==</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::::Garcia-Souza Luiz F(1,2), Cizmarova B(2,3), Sumbalova Z(2,4), Menz V(1), Gatterer H(1), Volani C(5), Lundby C(6), Burtscher M(1), Gnaiger E(2,7)</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::::Garcia-Souza Luiz F(1,2), Cizmarova B(2,3), Sumbalova Z(2,4), Menz V(1), Gatterer H(1), Volani C(5), Lundby C(6), Burtscher M(1), Gnaiger E(2,7)</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>::::#Inst Sport Science, Univ Innsbruck, Austria</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>::::# Inst Sport Science, Univ Innsbruck, Austria</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>::::#Daniel Swarovski Research Lab, Dept Visceral, Transplant Thoracic Surgery, Medical Univ Innsbruck, Austria</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>::::# Daniel Swarovski Research Lab, Dept Visceral, Transplant Thoracic Surgery, Medical Univ Innsbruck, Austria</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>::::#Dept Medical Clinical Biochem, Fac Medicine, Pavol Jozef Šafárik Univ Košice, Slovakia</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>::::# Dept Medical Clinical Biochem, Fac Medicine, Pavol Jozef Šafárik Univ Košice, Slovakia</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>::::#Pharmacobiochemical Lab, 3rd Dept Internal Medicine, Fac Medicine, Comenius Univ, Bratislava, Slovakia</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>::::# Pharmacobiochemical Lab, 3rd Dept Internal Medicine, Fac Medicine, Comenius Univ, Bratislava, Slovakia</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>::::#Dept Internal Medicine II, Medical Univ Innsbruck, Austria</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>::::# Dept Internal Medicine II, Medical Univ Innsbruck, Austria</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>::::#Center Physical Activity Research, Univ Hospital Copenhagen, Denmark</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>::::# Center Physical Activity Research, Univ Hospital Copenhagen, Denmark</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>::::#Oroboros Instruments, Innsbruck, Austria. – luiz.garcia@student.i-med.ac.at</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>::::# Oroboros Instruments, Innsbruck, Austria. – luiz.garcia@student.i-med.ac.at</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>::::::Supported by K-Regio project MitoFit (GSLF, ZS, VM) and Action Austria-Slovakia (BV). Contribution to European Union Framework Programme Horizon 2020 COST Action CA15203 [[MitoEAGLE]].</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>:::::: Supported by K-Regio project MitoFit (GSLF, ZS, VM) and Action Austria-Slovakia (BV). Contribution to European Union Framework Programme Horizon 2020 COST Action CA15203 [[MitoEAGLE]].</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== References ==</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== References ==</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>::::#Siebenmann C, Robach P, Lundby C (2017) Regulation of blood volume in lowlanders exposed to high altitude. J Appl Physiol, jap-00118.</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>::::# Siebenmann C, Robach P, Lundby C (2017) Regulation of blood volume in lowlanders exposed to high altitude. J Appl Physiol, jap-00118.</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>::::#Mazzeo RS (2008) Physiological responses to exercise at altitude. Sports Med 38:1-8.</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>::::# Mazzeo RS (2008) Physiological responses to exercise at altitude. Sports Med 38:1-8.</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>::::#Garcia-Souza LF, Cizmarova B, Sumbalova Z, Menz V, Burtscher M, Gnaiger E (2017) Assessment of mitochondrial respiratory function in cryopreserved platelets. Abstract, MiPschool Obergurgl 2017.</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>::::# Garcia-Souza LF, Cizmarova B, Sumbalova Z, Menz V, Burtscher M, Gnaiger E (2017) Assessment of mitochondrial respiratory function in cryopreserved platelets. Abstract, MiPschool Obergurgl 2017.</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>::::#Sumbalova Z, Hiller E, Chang S, Garcia L, Droescher S, Calabria E, Volani C, Krumschnabel G, Gnaiger E (2016) Isolation of blood cells for HRR. Mitochondr Physiol Network 21.17:1-15.</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>::::# Sumbalova Z, Hiller E, Chang S, Garcia L, Droescher S, Calabria E, Volani C, Krumschnabel G, Gnaiger E (2016) Isolation of blood cells for HRR. Mitochondr Physiol Network 21.17:1-15.</div></td></tr>
</table>
Gnaiger Erich
https://wiki.oroboros.at/index.php?title=Garcia-Souza_2017_MiP2017&diff=163256&oldid=prev
Beno Marija at 13:22, 1 October 2018
2018-10-01T13:22:35Z
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<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">← Older revision</td>
<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 13:22, 1 October 2018</td>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|title=[[Image:GarciaL.JPG|left|90px|Luiz Garcia]] Short-term acclimatization to moderate altitude does not affect respiration of peripheral blood mononuclear cells and platelets.</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|title=[[Image:GarciaL.JPG|left|90px|Luiz Garcia]] Short-term acclimatization to moderate altitude does not affect respiration of peripheral blood mononuclear cells and platelets.</div></td></tr>
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<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>|authors=Garcia-Souza Luiz F, <del style="font-weight: bold; text-decoration: none;">Velika </del>B, Sumbalova Z, Menz V, Gatterer H, Volani C, Lundby C, Burtscher M, Gnaiger E</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>|authors=Garcia-Souza Luiz F, <ins style="font-weight: bold; text-decoration: none;">Cizmarova </ins>B, Sumbalova Z, Menz V, Gatterer H, Volani C, Lundby C, Burtscher M, Gnaiger E</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|year=2017</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|year=2017</div></td></tr>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Affiliations and support ==</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Affiliations and support ==</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>::::Garcia-Souza Luiz F(1,2), <del style="font-weight: bold; text-decoration: none;">Velika </del>B(2,3), Sumbalova Z(2,4), Menz V(1), Gatterer H(1), Volani C(5), Lundby C(6), Burtscher M(1), Gnaiger E(2,7)</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>::::Garcia-Souza Luiz F(1,2), <ins style="font-weight: bold; text-decoration: none;">Cizmarova </ins>B(2,3), Sumbalova Z(2,4), Menz V(1), Gatterer H(1), Volani C(5), Lundby C(6), Burtscher M(1), Gnaiger E(2,7)</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::::#Inst Sport Science, Univ Innsbruck, Austria</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::::#Inst Sport Science, Univ Innsbruck, Austria</div></td></tr>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::::#Siebenmann C, Robach P, Lundby C (2017) Regulation of blood volume in lowlanders exposed to high altitude. J Appl Physiol, jap-00118.</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::::#Siebenmann C, Robach P, Lundby C (2017) Regulation of blood volume in lowlanders exposed to high altitude. J Appl Physiol, jap-00118.</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::::#Mazzeo RS (2008) Physiological responses to exercise at altitude. Sports Med 38:1-8.</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::::#Mazzeo RS (2008) Physiological responses to exercise at altitude. Sports Med 38:1-8.</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>::::#Garcia-Souza LF, <del style="font-weight: bold; text-decoration: none;">Velika </del>B, Sumbalova Z, Menz V, Burtscher M, Gnaiger E (2017) Assessment of mitochondrial respiratory function in cryopreserved platelets. Abstract, MiPschool Obergurgl 2017.</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>::::#Garcia-Souza LF, <ins style="font-weight: bold; text-decoration: none;">Cizmarova </ins>B, Sumbalova Z, Menz V, Burtscher M, Gnaiger E (2017) Assessment of mitochondrial respiratory function in cryopreserved platelets. Abstract, MiPschool Obergurgl 2017.</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::::#Sumbalova Z, Hiller E, Chang S, Garcia L, Droescher S, Calabria E, Volani C, Krumschnabel G, Gnaiger E (2016) Isolation of blood cells for HRR. Mitochondr Physiol Network 21.17:1-15.</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::::#Sumbalova Z, Hiller E, Chang S, Garcia L, Droescher S, Calabria E, Volani C, Krumschnabel G, Gnaiger E (2016) Isolation of blood cells for HRR. Mitochondr Physiol Network 21.17:1-15.</div></td></tr>
</table>
Beno Marija
https://wiki.oroboros.at/index.php?title=Garcia-Souza_2017_MiP2017&diff=144895&oldid=prev
Garcia Luiz at 13:24, 7 November 2017
2017-11-07T13:24:12Z
<p></p>
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<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 13:24, 7 November 2017</td>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>{{Abstract</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>{{Abstract</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>|title=[[Image:GarciaL.JPG|left|90px|Luiz Garcia]] Short-term <del style="font-weight: bold; text-decoration: none;">adaptation </del>to moderate altitude does not affect respiration of peripheral blood mononuclear cells and platelets.</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>|title=[[Image:GarciaL.JPG|left|90px|Luiz Garcia]] Short-term <ins style="font-weight: bold; text-decoration: none;">acclimatization </ins>to moderate altitude does not affect respiration of peripheral blood mononuclear cells and platelets.</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|info=[[MiP2017]]</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|info=[[MiP2017]]</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|authors=Garcia-Souza Luiz F, Velika B, Sumbalova Z, Menz V, Gatterer H, Volani C, Lundby C, Burtscher M, Gnaiger E</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|authors=Garcia-Souza Luiz F, Velika B, Sumbalova Z, Menz V, Gatterer H, Volani C, Lundby C, Burtscher M, Gnaiger E</div></td></tr>
</table>
Garcia Luiz
https://wiki.oroboros.at/index.php?title=Garcia-Souza_2017_MiP2017&diff=144854&oldid=prev
Garcia Luiz at 09:19, 7 November 2017
2017-11-07T09:19:05Z
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<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 09:19, 7 November 2017</td>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>{{Abstract</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>{{Abstract</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>|title=[[Image:GarciaL.JPG|left|90px|Luiz Garcia]] Short-term adaptation to <del style="font-weight: bold; text-decoration: none;">high </del>altitude does not affect respiration of peripheral blood mononuclear cells and platelets.</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>|title=[[Image:GarciaL.JPG|left|90px|Luiz Garcia]] Short-term adaptation to <ins style="font-weight: bold; text-decoration: none;">moderate </ins>altitude does not affect respiration of peripheral blood mononuclear cells and platelets.</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|info=[[MiP2017]]</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|info=[[MiP2017]]</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|authors=Garcia-Souza Luiz F, Velika B, Sumbalova Z, Menz V, Gatterer H, Volani C, Lundby C, Burtscher M, Gnaiger E</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|authors=Garcia-Souza Luiz F, Velika B, Sumbalova Z, Menz V, Gatterer H, Volani C, Lundby C, Burtscher M, Gnaiger E</div></td></tr>
</table>
Garcia Luiz
https://wiki.oroboros.at/index.php?title=Garcia-Souza_2017_MiP2017&diff=144802&oldid=prev
Beno Marija at 13:51, 3 November 2017
2017-11-03T13:51:56Z
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Beno Marija
https://wiki.oroboros.at/index.php?title=Garcia-Souza_2017_MiP2017&diff=144189&oldid=prev
Garcia Luiz at 09:35, 20 October 2017
2017-10-20T09:35:46Z
<p></p>
<table style="background-color: #fff; color: #202122;" data-mw="interface">
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<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 09:35, 20 October 2017</td>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|abstract=[[Image:MITOEAGLE-logo.jpg|left|100px|link=http://www.mitoglobal.org/index.php/MITOEAGLE|COST Action MITOEAGLE]] Peripheral-blood mononuclear cells (PBMC) and platelets (PLT) are potentially powerful models for the diagnosis of mitochondrial respiratory function and dysfunction, offering a minimally invasive approach in comparison to tissue biopsies. However, several variables that may interfere with mitochondrial physiology in blood cells are still unsolved. High altitude acclimatizaton induces whole body alterations due to low atmospheric oxygen levels and cold temperatures. A decrease of plasma volume, increase in arterial blood pressure and red blood cell count are some of the acclimatizations at altitude [1,2]. However, mitochondrial function of PBMC and PLT at short-term exposure to moderate altitude was never addressed. The objective of our study was the analysis of mitochondrial function of PBMC and PLT after a transition of healthy subjects to 2020 m altitude for 48 h.</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|abstract=[[Image:MITOEAGLE-logo.jpg|left|100px|link=http://www.mitoglobal.org/index.php/MITOEAGLE|COST Action MITOEAGLE]] Peripheral-blood mononuclear cells (PBMC) and platelets (PLT) are potentially powerful models for the diagnosis of mitochondrial respiratory function and dysfunction, offering a minimally invasive approach in comparison to tissue biopsies. However, several variables that may interfere with mitochondrial physiology in blood cells are still unsolved. High altitude acclimatizaton induces whole body alterations due to low atmospheric oxygen levels and cold temperatures. A decrease of plasma volume, increase in arterial blood pressure and red blood cell count are some of the acclimatizations at altitude [1,2]. However, mitochondrial function of PBMC and PLT at short-term exposure to moderate altitude was never addressed. The objective of our study was the analysis of mitochondrial function of PBMC and PLT after a transition of healthy subjects to 2020 m altitude for 48 h.</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>Blood was collected from <del style="font-weight: bold; text-decoration: none;">eight </del>healthy female volunteers (26.3 ± 5.7 years) at low altitude of 575 m (Innsbruck, Austria) for 4 days and exposed to an altitude of 2020 m (Kühtai, Austria) for 2 days. During the study, the participants were under a controlled diet and physical activity restricted to a light 1-h walk per day. Every morning at fasted state, blood was taken (18 mL) from up to 4 participants for measurements of respiration in freshly isolated blood cells. PBMC and PLT were separated from whole blood by centrifugation in Ficoll-Paque™ PLUS density medium using 50 mL Leucosep tubes [3]. After the first washing of the PBMC-PLT layer with DPBS (25 mL, 120 g, RT), the PBMC were washed two times with ice-cold DPBS+2% FBS (50 mL, 300 ''g'', 4 °C). Isolated cells were counted with a SYSMEX XN-350 haematology analyser. 3·10<sup>6</sup> PBMC or 150·10<sup>6</sup> PLT were added into the 2 mL chambers of the O2k-FluoRespirometer (Oroboros Instruments, Austria) containing mitochondrial respiration medium MiR05-Kit with catalase at 37 °C [3,4]. The coupling control & viability protocol (CCVP) was applied. Respiration of PBMC was corrected for the contribution by contaminating platelets.</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>Blood was collected from <ins style="font-weight: bold; text-decoration: none;">seven </ins>healthy female volunteers (26.3 ± 5.7 years) at low altitude of 575 m (Innsbruck, Austria) for 4 days and exposed to an altitude of 2020 m (Kühtai, Austria) for 2 days. During the study, the participants were under a controlled diet and physical activity restricted to a light 1-h walk per day. Every morning at fasted state, blood was taken (18 mL) from up to 4 participants for measurements of respiration in freshly isolated blood cells. PBMC and PLT were separated from whole blood by centrifugation in Ficoll-Paque™ PLUS density medium using 50 mL Leucosep tubes [3]. After the first washing of the PBMC-PLT layer with DPBS (25 mL, 120 g, RT), the PBMC were washed two times with ice-cold DPBS+2% FBS (50 mL, 300 ''g'', 4 °C). Isolated cells were counted with a SYSMEX XN-350 haematology analyser. 3·10<sup>6</sup> PBMC or 150·10<sup>6</sup> PLT were added into the 2 mL chambers of the O2k-FluoRespirometer (Oroboros Instruments, Austria) containing mitochondrial respiration medium MiR05-Kit with catalase at 37 °C [3,4]. The coupling control & viability protocol (CCVP) was applied. Respiration of PBMC was corrected for the contribution by contaminating platelets.</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>PBMC showed a ROUTINE respiration of 5.0 amol·s<sup>-1</sup>·cell<sup>-1</sup> at 575 m and 5.2 amol·s<sup>-1</sup>·cell<sup>-1</sup> at 2020 m. ET capacity was 18.8 amol·s<sup>-1</sup>·cell<sup>-1</sup> (575 m) and 17.4 amol·s<sup>-1</sup>·cell<sup>-1</sup> (2020 m). PLT showed a ROUTINE respiration of 0.12 amol·s<sup>-1</sup>·cell<sup>-1</sup> at 575 m and 0.11 amol·s<sup>-1</sup>·cell<sup>-1</sup> at 2020 m. ET capacity was 0.34 amol·s<sup>-1</sup>·cell<sup>-1</sup> (575 m) and 0.31 amol·s<sup>-1</sup>·cell<sup>-1</sup> (2020 m). There were no significant differences at altitude in PBMC and PLT. Flux control ratios in both cell types were not affected by altitude and cell viability was not compromised at altitude during sample transport to Innsbruck: viability was 94% and 93.3% in PBMC and 92.2% and 90.5% in PLT (575 m and 2020 m, respectively).</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>PBMC showed a ROUTINE respiration of 5.0 amol·s<sup>-1</sup>·cell<sup>-1</sup> at 575 m and 5.2 amol·s<sup>-1</sup>·cell<sup>-1</sup> at 2020 m. ET capacity was 18.8 amol·s<sup>-1</sup>·cell<sup>-1</sup> (575 m) and 17.4 amol·s<sup>-1</sup>·cell<sup>-1</sup> (2020 m). PLT showed a ROUTINE respiration of 0.12 amol·s<sup>-1</sup>·cell<sup>-1</sup> at 575 m and 0.11 amol·s<sup>-1</sup>·cell<sup>-1</sup> at 2020 m. ET capacity was 0.34 amol·s<sup>-1</sup>·cell<sup>-1</sup> (575 m) and 0.31 amol·s<sup>-1</sup>·cell<sup>-1</sup> (2020 m). There were no significant differences at altitude in PBMC and PLT. Flux control ratios in both cell types were not affected by altitude and cell viability was not compromised at altitude during sample transport to Innsbruck: viability was 94% and 93.3% in PBMC and 92.2% and 90.5% in PLT (575 m and 2020 m, respectively).</div></td></tr>
</table>
Garcia Luiz
https://wiki.oroboros.at/index.php?title=Garcia-Souza_2017_MiP2017&diff=144060&oldid=prev
Gnaiger Erich at 06:54, 20 October 2017
2017-10-20T06:54:14Z
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<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">← Older revision</td>
<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 06:54, 20 October 2017</td>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|year=2017</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|year=2017</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|event=MiP2017</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|event=MiP2017</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>|abstract=[[Image:MITOEAGLE-logo.jpg|left|100px|link=http://www.mitoglobal.org/index.php/MITOEAGLE|COST Action MITOEAGLE]] Peripheral-blood mononuclear cells (PBMC) and platelets (PLT) are potentially powerful models for the diagnosis of mitochondrial respiratory function and dysfunction, offering a minimally invasive approach in comparison to tissue biopsies. However, several variables that may interfere with mitochondrial physiology in blood cells are still unsolved. High altitude <del style="font-weight: bold; text-decoration: none;">experience </del>induces whole body <del style="font-weight: bold; text-decoration: none;">adaptations </del>due to low <del style="font-weight: bold; text-decoration: none;">O<sub>2</sub> concentration in the atmosphere </del>and cold <del style="font-weight: bold; text-decoration: none;">temperature</del>. <del style="font-weight: bold; text-decoration: none;">Decrease </del>of plasma volume, increase in arterial blood pressure and red blood <del style="font-weight: bold; text-decoration: none;">cells </del>count are some of the <del style="font-weight: bold; text-decoration: none;">adaptations regarding the blood stream adaptation to </del>altitude [1,2]. However, mitochondrial function of PBMC and PLT <del style="font-weight: bold; text-decoration: none;">under </del>altitude <del style="font-weight: bold; text-decoration: none;">adaptation </del>was never addressed. <del style="font-weight: bold; text-decoration: none;">Therefore, the </del>objective of our study <del style="font-weight: bold; text-decoration: none;">is to analyse </del>mitochondrial function of PBMC and PLT <del style="font-weight: bold; text-decoration: none;">exposed </del>to 2020 m altitude <del style="font-weight: bold; text-decoration: none;">adaptation during the first </del>48 <del style="font-weight: bold; text-decoration: none;">hours</del>.</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>|abstract=[[Image:MITOEAGLE-logo.jpg|left|100px|link=http://www.mitoglobal.org/index.php/MITOEAGLE|COST Action MITOEAGLE]] Peripheral-blood mononuclear cells (PBMC) and platelets (PLT) are potentially powerful models for the diagnosis of mitochondrial respiratory function and dysfunction, offering a minimally invasive approach in comparison to tissue biopsies. However, several variables that may interfere with mitochondrial physiology in blood cells are still unsolved. High altitude <ins style="font-weight: bold; text-decoration: none;">acclimatizaton </ins>induces whole body <ins style="font-weight: bold; text-decoration: none;">alterations </ins>due to low <ins style="font-weight: bold; text-decoration: none;">atmospheric oxygen levels </ins>and cold <ins style="font-weight: bold; text-decoration: none;">temperatures</ins>. <ins style="font-weight: bold; text-decoration: none;">A decrease </ins>of plasma volume, increase in arterial blood pressure and red blood <ins style="font-weight: bold; text-decoration: none;">cell </ins>count are some of the <ins style="font-weight: bold; text-decoration: none;">acclimatizations at </ins>altitude [1,2]. However, mitochondrial function of PBMC and PLT <ins style="font-weight: bold; text-decoration: none;">at short-term exposure to moderate </ins>altitude was never addressed. <ins style="font-weight: bold; text-decoration: none;">The </ins>objective of our study <ins style="font-weight: bold; text-decoration: none;">was the analysis of </ins>mitochondrial function of PBMC and PLT <ins style="font-weight: bold; text-decoration: none;">after a transition of healthy subjects </ins>to 2020 m altitude <ins style="font-weight: bold; text-decoration: none;">for </ins>48 <ins style="font-weight: bold; text-decoration: none;">h</ins>.</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>Blood was collected from eight healthy female volunteers (26.3 ± 5.7 years) at low altitude of 575 m (Innsbruck, Austria) for 4 days and exposed to altitude of 2020 m (Kühtai, Austria) for 2 days. During the study, the participants were under a controlled diet and physical activity restricted to a light 1-h walk per day. Every morning at fasted state, blood was taken (18 mL) from up to 4 participants for measurements of freshly isolated blood <del style="font-weight: bold; text-decoration: none;">cell respiration</del>. PBMC and PLT were separated from whole blood by centrifugation <del style="font-weight: bold; text-decoration: none;">on </del>Ficoll-Paque™ PLUS density medium using 50 mL Leucosep tubes [3]. After the first washing of PBMC-<del style="font-weight: bold; text-decoration: none;">platelets </del>layer with DPBS (25 mL, 120 g, RT), the PBMC were washed two times with ice-cold DPBS+2% FBS (50 mL, 300 g, 4 °C). Isolated cells were counted <del style="font-weight: bold; text-decoration: none;">on </del>SYSMEX XN-350 haematology analyser. <del style="font-weight: bold; text-decoration: none;">3·106 </del>PBMC or <del style="font-weight: bold; text-decoration: none;">150·106 </del>PLT were added into the 2 mL chambers of the O2k-FluoRespirometer (Oroboros Instruments<del style="font-weight: bold; text-decoration: none;">, Innsbruck</del>, Austria) containing mitochondrial respiration medium MiR05-Kit with catalase at 37 °C [3,4]. The coupling control & viability protocol (CCVP) was applied. Respiration of PBMC was corrected for the contribution by contaminating platelets.</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>Blood was collected from eight healthy female volunteers (26.3 ± 5.7 years) at low altitude of 575 m (Innsbruck, Austria) for 4 days and exposed to <ins style="font-weight: bold; text-decoration: none;">an </ins>altitude of 2020 m (Kühtai, Austria) for 2 days. During the study, the participants were under a controlled diet and physical activity restricted to a light 1-h walk per day. Every morning at fasted state, blood was taken (18 mL) from up to 4 participants for measurements of <ins style="font-weight: bold; text-decoration: none;">respiration in </ins>freshly isolated blood <ins style="font-weight: bold; text-decoration: none;">cells</ins>. PBMC and PLT were separated from whole blood by centrifugation <ins style="font-weight: bold; text-decoration: none;">in </ins>Ficoll-Paque™ PLUS density medium using 50 mL Leucosep tubes [3]. After the first washing of <ins style="font-weight: bold; text-decoration: none;">the </ins>PBMC-<ins style="font-weight: bold; text-decoration: none;">PLT </ins>layer with DPBS (25 mL, 120 g, RT), the PBMC were washed two times with ice-cold DPBS+2% FBS (50 mL, 300 <ins style="font-weight: bold; text-decoration: none;">''</ins>g<ins style="font-weight: bold; text-decoration: none;">''</ins>, 4 °C). Isolated cells were counted <ins style="font-weight: bold; text-decoration: none;">with a </ins>SYSMEX XN-350 haematology analyser. <ins style="font-weight: bold; text-decoration: none;">3·10<sup>6</sup> </ins>PBMC or <ins style="font-weight: bold; text-decoration: none;">150·10<sup>6</sup> </ins>PLT were added into the 2 mL chambers of the O2k-FluoRespirometer (Oroboros Instruments, Austria) containing mitochondrial respiration medium MiR05-Kit with catalase at 37 °C [3,4]. The coupling control & viability protocol (CCVP) was applied. Respiration of PBMC was corrected for the contribution by contaminating platelets.</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>PBMC showed a ROUTINE respiration of 5.0 amol·s<sup>-1</sup>·cell<sup>-1</sup> at 575 m and 5.2 amol·s<sup>-1</sup>·cell<sup>-1</sup> at 2020 m. ET capacity was 18.8 amol·s<sup>-1</sup>·cell<sup>-1</sup> (575 m) and 17.4 amol·s<sup>-1</sup>·cell<sup>-1</sup> (2020 m). PLT showed a ROUTINE respiration of 0.12 amol·s<sup>-1</sup>·cell<sup>-1</sup> at 575 m and 0.11 amol·s<sup>-1</sup>·cell<sup>-1</sup> at 2020 m. ET capacity was 0.34 amol·s<sup>-1</sup>·cell<sup>-1</sup> (575 m) and 0.31 amol·s<sup>-1</sup>·cell<sup>-1</sup> (2020 m). There were no significant differences <del style="font-weight: bold; text-decoration: none;">in </del>altitude <del style="font-weight: bold; text-decoration: none;">adaptation </del>in PBMC and PLT. Flux control ratios in both cell types were not affected by altitude <del style="font-weight: bold; text-decoration: none;">adaptation </del>and cell viability was not compromised <del style="font-weight: bold; text-decoration: none;">from </del>altitude <del style="font-weight: bold; text-decoration: none;">effect or any apparent complication </del>during sample transport<del style="font-weight: bold; text-decoration: none;">, </del>94% and 93.3% in PBMC <del style="font-weight: bold; text-decoration: none;">(575 m and 2020 m, respectively) </del>and 92.2% and 90.5% in PLT (575 m and 2020 m, respectively).</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>PBMC showed a ROUTINE respiration of 5.0 amol·s<sup>-1</sup>·cell<sup>-1</sup> at 575 m and 5.2 amol·s<sup>-1</sup>·cell<sup>-1</sup> at 2020 m. ET capacity was 18.8 amol·s<sup>-1</sup>·cell<sup>-1</sup> (575 m) and 17.4 amol·s<sup>-1</sup>·cell<sup>-1</sup> (2020 m). PLT showed a ROUTINE respiration of 0.12 amol·s<sup>-1</sup>·cell<sup>-1</sup> at 575 m and 0.11 amol·s<sup>-1</sup>·cell<sup>-1</sup> at 2020 m. ET capacity was 0.34 amol·s<sup>-1</sup>·cell<sup>-1</sup> (575 m) and 0.31 amol·s<sup>-1</sup>·cell<sup>-1</sup> (2020 m). There were no significant differences <ins style="font-weight: bold; text-decoration: none;">at </ins>altitude in PBMC and PLT. Flux control ratios in both cell types were not affected by altitude and cell viability was not compromised <ins style="font-weight: bold; text-decoration: none;">at </ins>altitude during sample transport <ins style="font-weight: bold; text-decoration: none;">to Innsbruck: viability was </ins>94% and 93.3% in PBMC and 92.2% and 90.5% in PLT (575 m and 2020 m, respectively).</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>In summary, PBMC and PLT respiration was not affected during the first 48 <del style="font-weight: bold; text-decoration: none;">hours of </del>altitude <del style="font-weight: bold; text-decoration: none;">experience</del>. <del style="font-weight: bold; text-decoration: none;">According </del>to <del style="font-weight: bold; text-decoration: none;">our results, </del>altitude <del style="font-weight: bold; text-decoration: none;">adaptation will </del>not <del style="font-weight: bold; text-decoration: none;">be </del>a variable which interferes <del style="font-weight: bold; text-decoration: none;">in </del>mitochondrial <del style="font-weight: bold; text-decoration: none;">function </del>diagnostics in pathological states <del style="font-weight: bold; text-decoration: none;">during the first 48 hours of altitude exposure</del>. However, longer exposure of <del style="font-weight: bold; text-decoration: none;">altitude adaptation or </del>higher altitude exposure should be investigated.</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>In summary, PBMC and PLT respiration was not affected during the first 48 <ins style="font-weight: bold; text-decoration: none;">h at </ins>altitude. <ins style="font-weight: bold; text-decoration: none;">Our results suggest that short-term expsure </ins>to <ins style="font-weight: bold; text-decoration: none;">mild </ins>altitude <ins style="font-weight: bold; text-decoration: none;">is </ins>not a variable which interferes <ins style="font-weight: bold; text-decoration: none;">with </ins>mitochondrial <ins style="font-weight: bold; text-decoration: none;">functional </ins>diagnostics in pathological states. However, longer exposure <ins style="font-weight: bold; text-decoration: none;">to altitude and the effect </ins>of higher altitude exposure should be investigated.</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|editor=[[Kandolf G]]</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|editor=[[Kandolf G]]</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|mipnetlab=AT Innsbruck Oroboros, SK Bratislava Sumbalova Z, AT Innsbruck Burtscher M, CH Zurich Lundby C, CH Zurich University of Zurich Physiology, DK Copenhagen Lundby C, AT Innsbruck Gnaiger E</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|mipnetlab=AT Innsbruck Oroboros, SK Bratislava Sumbalova Z, AT Innsbruck Burtscher M, CH Zurich Lundby C, CH Zurich University of Zurich Physiology, DK Copenhagen Lundby C, AT Innsbruck Gnaiger E</div></td></tr>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::::#Oroboros Instruments, Innsbruck, Austria. – luiz.garcia@student.i-med.ac.at</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>::::#Oroboros Instruments, Innsbruck, Austria. – luiz.garcia@student.i-med.ac.at</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>::::::Supported by K-Regio project MitoFit (GSLF, ZS, VM) and Action Austria-Slovakia (BV). Contribution to European Union Framework Programme Horizon 2020 COST Action CA15203 [[<del style="font-weight: bold; text-decoration: none;">MITOEAGLE</del>]].</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>::::::Supported by K-Regio project MitoFit (GSLF, ZS, VM) and Action Austria-Slovakia (BV). Contribution to European Union Framework Programme Horizon 2020 COST Action CA15203 [[<ins style="font-weight: bold; text-decoration: none;">MitoEAGLE</ins>]].</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== References ==</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== References ==</div></td></tr>
</table>
Gnaiger Erich