Garedew 2005 Eur Shock Soc: Difference between revisions
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|area=Respiration, Instruments and methods | |||
|organism=Human | |organism=Human | ||
|model cell lines=Fibroblast | |model cell lines=Fibroblast | ||
|preparations=Intact cells | |preparations=Intact cells | ||
|topics=Uncoupler | |||
|couplingstates=OXPHOS | |couplingstates=OXPHOS | ||
|instruments=Oxygraph-2k, TIP2k | |instruments=Oxygraph-2k, TIP2k | ||
|additional=Instrumental and methodological aspects | |additional=Instrumental and methodological aspects | ||
|discipline=Mitochondrial Physiology | |discipline=Mitochondrial Physiology | ||
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Revision as of 13:20, 11 August 2013
Garedew A, HΓΌtter E, Haffner B, Gradl P, Gradl L, Jansen-DΓΌrr P, Gnaiger E (2005) High-resolution respirometry for the study of mitochondrial function in health and disease. The OROBOROS Oxygraph-2k. Proc 11th Congress Eur Shock Soc, Vienna, Austria (Redl H, ed) Medimond, Bologna: 107-111. |
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Garedew A, Huetter E, Haffner B, Gradl P, Gradl L, Jansen-Duerr P, Gnaiger E (2005) Medimond
Abstract: High-resolution respirometry with mitochondria, cells or biopsies is based on the unique features of the OROBOROS Oxygraph-2k, combining instrumental design, electronics and task-specific DatLab software. Various respiratory states are analyzed with <0.4 million cells or <4 mg biopsy (muscle, liver) per assay. Complex substrate/inhibitor titration protocols are used to quantify functional properties of selected components of the respiratory chain. The electronic titration-injection micropump TIP2k is applied for kinetics as a modular component of the Oxygraph-2k. DatLab 4 provides on-line instrumental background corrections of oxygen flux and on-line data analysis, and tabulated results are available at the end of an experiment. This sets a new standard for biomedical and clinical studies, combining high-resolution with instant diagnostic information.
β’ O2k-Network Lab: AT_Innsbruck_Gnaiger E, AT_Innsbruck_Jansen-Duerr P, AT Innsbruck MitoCom
Labels: MiParea: Respiration, Instruments and methods"Instruments and methods" is not in the list (Respiration, Instruments;methods, mt-Biogenesis;mt-density, mt-Structure;fission;fusion, mt-Membrane, mtDNA;mt-genetics, nDNA;cell genetics, Genetic knockout;overexpression, Comparative MiP;environmental MiP, Gender, ...) of allowed values for the "MiP area" property.
Organism: Human
Preparation: Intact cells
Regulation: Uncoupler Coupling state: OXPHOS
HRR: Oxygraph-2k, TIP2k
Instrumental and methodological aspects