Garedew 2005 Eur Shock Soc: Difference between revisions
No edit summary |
No edit summary |
||
Line 7: | Line 7: | ||
|abstract=[[High-resolution respirometry]] with mitochondria, cells or biopsies is based on the unique features of the OROBOROS [[Oxygraph-2k]], combining instrumental design, electronics and task-specific DatLab software. | |abstract=[[High-resolution respirometry]] with mitochondria, cells or biopsies is based on the unique features of the OROBOROS [[Oxygraph-2k]], combining instrumental design, electronics and task-specific DatLab software. | ||
Various respiratory states are analyzed with <0.4 million cells or <4 mg biopsy (muscle, liver) per assay. Complex substrate/inhibitor titration protocols are used to quantify functional properties of selected components of the respiratory chain. The electronic titration-injection micropump [[TIP2k]] is applied for kinetics as a modular component of the Oxygraph-2k. [[DatLab]] 4 provides on-line instrumental background corrections of oxygen flux and on-line data analysis, and tabulated results are available at the end of an experiment. This sets a new standard for biomedical and clinical studies, combining high-resolution with instant diagnostic information. | Various respiratory states are analyzed with <0.4 million cells or <4 mg biopsy (muscle, liver) per assay. Complex substrate/inhibitor titration protocols are used to quantify functional properties of selected components of the respiratory chain. The electronic titration-injection micropump [[TIP2k]] is applied for kinetics as a modular component of the Oxygraph-2k. [[DatLab]] 4 provides on-line instrumental background corrections of oxygen flux and on-line data analysis, and tabulated results are available at the end of an experiment. This sets a new standard for biomedical and clinical studies, combining high-resolution with instant diagnostic information. | ||
|mipnetlab=AT_Innsbruck_Gnaiger E, AT_Innsbruck_Jansen-Duerr P, AT Innsbruck | |mipnetlab=AT_Innsbruck_Gnaiger E, AT_Innsbruck_Jansen-Duerr P, AT Innsbruck OROBOROS | ||
|discipline=Mitochondrial Physiology | |discipline=Mitochondrial Physiology | ||
}} | }} | ||
Line 18: | Line 18: | ||
|couplingstates=OXPHOS | |couplingstates=OXPHOS | ||
|instruments=Oxygraph-2k, TIP2k | |instruments=Oxygraph-2k, TIP2k | ||
|discipline=Mitochondrial Physiology | |discipline=Mitochondrial Physiology | ||
}} | }} |
Revision as of 06:56, 16 August 2013
Garedew A, HΓΌtter E, Haffner B, Gradl P, Gradl L, Jansen-DΓΌrr P, Gnaiger E (2005) High-resolution respirometry for the study of mitochondrial function in health and disease. The OROBOROS Oxygraph-2k. Proc 11th Congress Eur Shock Soc, Vienna, Austria (Redl H, ed) Medimond, Bologna: 107-111. |
Β» Weblink
Garedew A, Huetter E, Haffner B, Gradl P, Gradl L, Jansen-Duerr P, Gnaiger E (2005) Medimond
Abstract: High-resolution respirometry with mitochondria, cells or biopsies is based on the unique features of the OROBOROS Oxygraph-2k, combining instrumental design, electronics and task-specific DatLab software. Various respiratory states are analyzed with <0.4 million cells or <4 mg biopsy (muscle, liver) per assay. Complex substrate/inhibitor titration protocols are used to quantify functional properties of selected components of the respiratory chain. The electronic titration-injection micropump TIP2k is applied for kinetics as a modular component of the Oxygraph-2k. DatLab 4 provides on-line instrumental background corrections of oxygen flux and on-line data analysis, and tabulated results are available at the end of an experiment. This sets a new standard for biomedical and clinical studies, combining high-resolution with instant diagnostic information.
β’ O2k-Network Lab: AT_Innsbruck_Gnaiger E, AT_Innsbruck_Jansen-Duerr P, AT Innsbruck OROBOROS
Labels: MiParea: Respiration, Instruments;methods
Organism: Human
Preparation: Intact cells
Regulation: Uncoupler Coupling state: OXPHOS
HRR: Oxygraph-2k, TIP2k