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Difference between revisions of "Gnaiger 2012 Abstract Bioblast-Gentle Science"

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Since a [http://www.oroboros.at/?Mitopathways-group 2007 workshop in Schroecken] and the first [http://www.mitophysiology.org/?MiP-summerschool2007 MiPsummer school 2007], advanced SUIT protocols for sequential activation particularly of CI-, CI+II- and CII-linked respiration are explained in the '[[Gnaiger 2007 MitoPathways|Blue Book]]'. It is not in the spirit of Gentle Science, however, to receive enthusiastic feedback by Emails and then read in a publication (2008) "''protocols developed by us''" without citation.  
Since a [http://www.oroboros.at/?Mitopathways-group 2007 workshop in Schroecken] and the first [http://www.mitophysiology.org/?MiP-summerschool2007 MiPsummer school 2007], advanced SUIT protocols for sequential activation particularly of CI-, CI+II- and CII-linked respiration are explained in the '[[Gnaiger 2007 MitoPathways|Blue Book]]'. It is not in the spirit of Gentle Science, however, to receive enthusiastic feedback by Emails and then read in a publication (2008) "''protocols developed by us''" without citation.  


More than 800 publications refer to the OROBOROS Oxygraph-2k in the methods section, reflecting the increasing interest in mitochondrial physiology. Reference to original publications on the O2k and the concept of high-resolution respirometry, however, are rare compared to the frequently written phrase “as described before”.  
More than 800 publications refer to the OROBOROS Oxygraph-2k in the methods section, reflecting the increasing interest in mitochondrial physiology. Reference to original publications on the O2k and the concept of high-resolution respirometry, however, are rare compared to the frequently written phrase “''as described before''”.  


The Bioblast Conference should allow open exchange of results and ideas in an environment inspired by art, music and friendship. Gentle Science is an illusion worthwhile to maintain.
The Bioblast Conference should allow open exchange of results and ideas in an environment inspired by art, music and friendship. Gentle Science is an illusion worthwhile to maintain.

Revision as of 06:35, 23 October 2012

Gnaiger E (2012) Gentle Science and the Bioblast Conference. Mitochondr Physiol Network 17.12.

Link: MiPNet17.12 Bioblast Conference - Open Access

Gnaiger E (2012)

Event: Bioblast Conference on Mitochondrial Competence

Erich Gnaiger

The concept of Gentle Science has been a driving force for more than 20 years of continuous development of high-resolution respirometry, which was initiated in a FWF project (1989). Our first generation Oxygraph was commercially available in 1992. A series of applications was presented at a BTK conference in 1994, and a full description of the instrument, method and concept of high-resolution respirometry was published in 1995 [1], when the positive feedback from early users had confirmed the validity of our approach [2]. The concept of O2k-MultiSensor extensions was implemented in the second generation Oxygraph-2k, developed in close cooperation with Phlipp Gradl (WGT-Elektronik) and distributed since 2002.

The strategy of OROBOROS INSTRUMENTS included the close link between university and company, teaching and training with Open Access to our published [3] and unpublished scientific background information, detailed descriptions of O2k-Protocols on our website, and direct communication with our customers and O2k-users (Mitochondrial Physiology Network).

Conforming to the OROBOROS logo we are proud to receive highly positive O2k-Feedback, but also provide help with O2k-Troubleshooting in open discussions. For the development of mitochondrial physiology and functional diagnosis in mitochondrial medicine, standardization of methods and protocols is an important yet elusive topic. Our O2k-Workshops contribute to a more general understanding of the rationale for standardized protocols, while the Bioblast wiki website (launched in 2010) may help to develop a more consistent terminology in mitochondrial physiology.

Since a 2007 workshop in Schroecken and the first MiPsummer school 2007, advanced SUIT protocols for sequential activation particularly of CI-, CI+II- and CII-linked respiration are explained in the 'Blue Book'. It is not in the spirit of Gentle Science, however, to receive enthusiastic feedback by Emails and then read in a publication (2008) "protocols developed by us" without citation.

More than 800 publications refer to the OROBOROS Oxygraph-2k in the methods section, reflecting the increasing interest in mitochondrial physiology. Reference to original publications on the O2k and the concept of high-resolution respirometry, however, are rare compared to the frequently written phrase “as described before”.

The Bioblast Conference should allow open exchange of results and ideas in an environment inspired by art, music and friendship. Gentle Science is an illusion worthwhile to maintain.

  1. Gnaiger E, Steinlechner-Maran R, Méndez G, Eberl T, Margreiter R (1995) Control of mitochondrial and cellular respiration by oxygen. J Bioenerg Biomembr 27: 583-596.
  2. Gnaiger E (2001) Bioenergetics at low oxygen: dependence of respiration and phosphorylation on oxygen and adenosine diphosphate supply. Respir Physiol 128: 277-297.
  3. Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopisies of human muscle. Methods Mol Biol 810: 25-58.

Keywords: High-resolution respirometry, Gentle Science, Oxygraph-2k

O2k-Network Lab: AT Innsbruck OROBOROS, AT Innsbruck Gnaiger E


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HRR: Oxygraph-2k, MiPNet-Publication"MiPNet-Publication" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property. 



Affiliations and author contributions

OROBOROS INSTRUMENTS, Innsbruck, Austria; and D. Swarovski Research Laboratory, Department of Visceral, Transplant and Thoracic Surgery, Medical University of Innsbruck, Austria. Email: [email protected]

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