Difference between revisions of "Goo 2013 Clin Exp Pharmacol Physiol"
(Created page with "{{Publication |title=Goo S, Pham T, Han JC, Nielsen P, Taberner A, Hickey A, Loiselle D (2013) Multiscale measurement of cardiac energetics. Clin Exp Pharmacol Physiol 40:671-681...") |
|||
| Line 1: | Line 1: | ||
{{Publication | {{Publication | ||
|title=Goo S, Pham T, Han JC, Nielsen P, Taberner A, Hickey A, Loiselle D (2013) Multiscale measurement of cardiac energetics. Clin Exp Pharmacol Physiol 40:671-681. | |title=Goo S, Pham T, Han JC, Nielsen P, Taberner A, Hickey A, Loiselle D (2013) Multiscale measurement of cardiac energetics. Clin Exp Pharmacol Physiol 40:671-681. | ||
|info=[http://www.ncbi.nlm.nih.gov/pubmed/23745944 PMID: 23745944] | |info=[http://www.ncbi.nlm.nih.gov/pubmed/23745944 PMID: 23745944] | ||
|authors=Goo S, Pham T, Han JC, Nielsen P, Taberner A, Hickey A, Loiselle D | |authors=Goo S, Pham T, Han JC, Nielsen P, Taberner A, Hickey A, Loiselle D | ||
|year=2013 | |year=2013 | ||
|journal=Clin Exp Pharmacol Physiol | |journal=Clin Exp Pharmacol Physiol | ||
|abstract=Herein we describe our laboratories' experimental methods for interrogating cardiac energetics at the organ (whole heart), tissue (trabecula) and perforated fibre (mitochondrial) levels. In whole heart and trabecula experiments, we focus on measuring pressure-volume (force-length) work and oxygen consumption (heat production) from which mechanical efficiency is derived. In both preparations (i.e. across scales differing by three orders of magnitude) we find efficiency values of 10%-15%. Mitochondrial experiments invoke a trio of titration protocols to yield information on oxygen consumption, ATP flux, membrane potential, electron leak and reactive oxygen species production, the latter two of which index energy transfer inefficiencies. | |abstract=Herein we describe our laboratories' experimental methods for interrogating cardiac energetics at the organ (whole heart), tissue (trabecula) and perforated fibre (mitochondrial) levels. In whole heart and trabecula experiments, we focus on measuring pressure-volume (force-length) work and oxygen consumption (heat production) from which mechanical efficiency is derived. In both preparations (i.e. across scales differing by three orders of magnitude) we find efficiency values of 10%-15%. Mitochondrial experiments invoke a trio of titration protocols to yield information on oxygen consumption, ATP flux, membrane potential, electron leak and reactive oxygen species production, the latter two of which index energy transfer inefficiencies. | ||
|keywords=ATP flux, cardiac enthalpy production, cardiac heat production, mitochondrial membrane potential, mitochondrial proton leak, mitochondrial reactive oxygen species production | |keywords=ATP flux, cardiac enthalpy production, cardiac heat production, mitochondrial membrane potential, mitochondrial proton leak, mitochondrial reactive oxygen species production | ||
}} | }} | ||
{{Labeling | {{Labeling | ||
|area=Respiration | |||
|couplingstates=LEAK, OXPHOS, ETS | |||
|substratestates=CI, CI+II | |||
|instruments=Oxygraph-2k | |instruments=Oxygraph-2k | ||
}} | }} | ||
Revision as of 12:46, 3 February 2014
| Goo S, Pham T, Han JC, Nielsen P, Taberner A, Hickey A, Loiselle D (2013) Multiscale measurement of cardiac energetics. Clin Exp Pharmacol Physiol 40:671-681. |
Goo S, Pham T, Han JC, Nielsen P, Taberner A, Hickey A, Loiselle D (2013) Clin Exp Pharmacol Physiol
Abstract: Herein we describe our laboratories' experimental methods for interrogating cardiac energetics at the organ (whole heart), tissue (trabecula) and perforated fibre (mitochondrial) levels. In whole heart and trabecula experiments, we focus on measuring pressure-volume (force-length) work and oxygen consumption (heat production) from which mechanical efficiency is derived. In both preparations (i.e. across scales differing by three orders of magnitude) we find efficiency values of 10%-15%. Mitochondrial experiments invoke a trio of titration protocols to yield information on oxygen consumption, ATP flux, membrane potential, electron leak and reactive oxygen species production, the latter two of which index energy transfer inefficiencies. • Keywords: ATP flux, cardiac enthalpy production, cardiac heat production, mitochondrial membrane potential, mitochondrial proton leak, mitochondrial reactive oxygen species production
Labels: MiParea: Respiration
Coupling state: LEAK, OXPHOS, ETS"ETS" is not in the list (LEAK, ROUTINE, OXPHOS, ET) of allowed values for the "Coupling states" property.
HRR: Oxygraph-2k
