Difference between revisions of "Gregori 2002 Methods Cell Sci"
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|additional=Environmental Physiology; Toxicology | |additional=Environmental Physiology; Toxicology | ||
|discipline=Environmental Physiology; Toxicology | |discipline=Environmental Physiology; Toxicology | ||
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Revision as of 14:48, 27 November 2012
| Grégori G, Denis M, Lefèvre D, Beker B (2002) A flow cytometric approach to assess phytoplankton respiration. Methods Cell Sci 24: 99-106. |
Gregori G, Denis M, Lefevre D, Beker B (2002) Methods Cell Sci
Abstract: Microbial respiration in the ocean is considered as the major process representative of the organic matter biological oxidation.The corresponding metabolic CO2 production was estimated to be about 22 Pg C y-1 [24]. However, the in situ respiration rate is generally too low (by several orders of magnitude) to be accessible to the available direct measurement methods. Some fluorescent probes, such as DiOC6(3) (Molecular Probes, USA) have been shown to be very sensitive to changes in the proton electrochemical potential difference (Δ µ~H+), characterising mitochondrial and plasmic membranes bearing the cell respiratory system in eukaryotic and prokaryotic cells respectively [22, 37]. In mitochondria, Δ µ~H+ is linked to the flux of oxygen uptake by a linear relationship [32]. To our knowledge, no such relationship has been established in the case of whole marine cells. In the present work, we addressed the dark respiration rate of the Chlorophyceae Dunaliella tertiolecta (Butcher) in axenic cultures, both directly by using a highly sensitive oxygraph (Oroboros) and by staining cells with DiOC6(3). We found and standardized a linear relationship between oxygen uptake by D. tertiolecta and its green fluorescence inducedby DiOC6(3), enabling the determination by flow cytometry of the respiration rate of D. tertiolecta. • Keywords: Aquatic microorganisms, Cell respiration, DiOC6(3), Flow cytometry
• O2k-Network Lab: FR_Marseille_Denis M, FR_Marseille_Gregori G
Labels:
Organism: Bacteria"Bacteria" is not in the list (Human, Pig, Mouse, Rat, Guinea pig, Bovines, Horse, Dog, Rabbit, Cat, ...) of allowed values for the "Mammal and model" property.
Preparation: Intact Cell; Cultured; Primary"Intact Cell; Cultured; Primary" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property.
Coupling state: OXPHOS
HRR: Oxygraph-2k
Environmental Physiology; Toxicology
