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== Affiliations ==
Dept Biol Sc, Louisiana State Univ, Baton Rouge, USA. - shand@LSU.edu.
== Abstract continued ==
LEA proteins and trehalose stabilize liposomes that mimicked biological membranes when desiccated. Neither the cytoplasmic-localized AfrLEA2 nor the mitochondrial-targeted AfrLEA3m exhibits preferential protection of one compositional type of liposome over another. Matrix-resident AfrLEA3m is not more proficient at stabilizing IMM-like liposomes that contain cardiolipin than is AfrLEA2. When trehalose and LEA proteins are used in combination, IMM-like liposomes and ILPM liposomes are protected to a significantly greater degree than when dried with either protectant alone. Modeling of AfrLEA2 and AfrLEA3m as α-helices shows arrangements of charged amino acids that are consistent with other amphipathic proteins capable of direct interaction with lipid bilayers.
== Figures ==
[[Image:MiP2015 Hand Figure.JPG|left|650px]]
Figure 1. Carboxyfluorescein leakage from liposomes dried overnight and rehydrated in the presence of LEA proteins or a control protein (lysozyme). Data represents the mean ± SD of ''n'' = 6 samples.
Figure 2. (A) Two views of AfrLEA3m modeled as an α-helix. Charged amino acids are depicted in red (acidic: D or E) or blue (basic: H, K, or R). Hydrophobic residues (A, G, I, L, M, V, or W) are colored gray and hydrophilic residues (N, Q, S, T, or Y) are depicted as yellow. (B) The α-helical backbone (white) is depicted with the charged residues (colored as above) between positions 45-219. (C) End-on view of residues 149-240 with only the charged amino acids visible.
== References and acknowledgements ==
#Hand SC, Menze MA (2015) Molecular approaches for improving desiccation tolerance: insights from the brine shrimp ''Artemia franciscana''. Planta 242:379-88.
#Abazari A, Chakraborty N, Hand SC, Aksan A, Toner M (2014) A Raman micro-spectroscopy study of water and trehalose in spin-dried cells. Biophysical J. 107:2253-62.
#Boswell LC, Menze MA, Hand SC (2014) Group 3 LEA proteins from embryos of ''Artemia franciscana'': Structural properties and protective abilities during desiccation. Physiol Biochem Zool 87:640-51.
#Boswell LC, Hand SC (2014) Intracellular localization of group 3 LEA proteins in embryos of ''Artemia franciscana''. Tissue and Cell 46:514-19.
Supported by National Science Foundation grants IOS-0920254 and IOS-1457061/IOS-1456809
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