Difference between revisions of "Hirzel 2013 J Recept Signal Transduct Res"
(Created page with "{{Publication |title=Hirzel E, Lindinger PW, Maseneni S, Giese M, Rhein VV, Eckert A, Hoch M, KrĂ€henbĂŒhl S, Eberle AN (2013) Differential modulation of ROS signals and other mi...")  |
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{{Publication | {{Publication | ||
|title=Hirzel E, Lindinger PW, Maseneni S, Giese M, Rhein VV, Eckert A, Hoch M, KrĂ€henbĂŒhl S, Eberle AN (2013) Differential modulation of ROS signals and other mitochondrial parameters by the antioxidants MitoQ, resveratrol and curcumin in human adipocytes. J Recept Signal Transduct Res 33:304-312.  | |title=Hirzel E, Lindinger PW, Maseneni S, Giese M, Rhein VV, Eckert A, Hoch M, KrĂ€henbĂŒhl S, Eberle AN (2013) Differential modulation of ROS signals and other mitochondrial parameters by the antioxidants MitoQ, resveratrol and curcumin in human adipocytes. J Recept Signal Transduct Res 33:304-312. | ||
|info=[http://www.ncbi.nlm.nih.gov/pubmed/23914782 PMID: 2391782] | |info=[http://www.ncbi.nlm.nih.gov/pubmed/23914782 PMID: 2391782] | ||
|authors=Hirzel E, Lindinger PW, Maseneni S, Giese M, Rhein VV, Eckert A, Hoch M, KrĂ€henbĂŒhl S, Eberle AN | |authors=Hirzel E, Lindinger PW, Maseneni S, Giese M, Rhein VV, Eckert A, Hoch M, KrĂ€henbĂŒhl S, Eberle AN | ||
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|journal=J Recept Signal Transduct Res | |journal=J Recept Signal Transduct Res | ||
|abstract=Mitochondrial reactive oxygen species (ROS) have been demonstrated to play an important role as signaling and regulating molecules in human adipocytes. In order to evaluate the differential modulating roles of antioxidants, we treated human adipocytes differentiated from human bone marrow-derived mesenchymal stem cells with MitoQ, resveratrol and curcumin. The effects on ROS, viability, mitochondrial respiration and intracellular ATP levels were examined. MitoQ lowered both oxidizing and reducing ROS. Resveratrol decreased reducing and curcumin oxidizing radicals only. All three substances slightly decreased state III respiration immediately after addition. After 24âh of treatment, MitoQ inhibited both basal and uncoupled oxygen consumption, whereas curcumin and resveratrol had no effect. Intracellular ATP levels were not altered. This demonstrates that MitoQ, resveratrol and curcumin exert potent modulating effects on ROS signaling in human adipocyte with marginal effects on metabolic parameters. | |abstract=Mitochondrial reactive oxygen species (ROS) have been demonstrated to play an important role as signaling and regulating molecules in human adipocytes. In order to evaluate the differential modulating roles of antioxidants, we treated human adipocytes differentiated from human bone marrow-derived mesenchymal stem cells with MitoQ, resveratrol and curcumin. The effects on ROS, viability, mitochondrial respiration and intracellular ATP levels were examined. MitoQ lowered both oxidizing and reducing ROS. Resveratrol decreased reducing and curcumin oxidizing radicals only. All three substances slightly decreased state III respiration immediately after addition. After 24âh of treatment, MitoQ inhibited both basal and uncoupled oxygen consumption, whereas curcumin and resveratrol had no effect. Intracellular ATP levels were not altered. This demonstrates that MitoQ, resveratrol and curcumin exert potent modulating effects on ROS signaling in human adipocyte with marginal effects on metabolic parameters. | ||
|keywords=Adipocyte, antioxidant, curcumin, human mesenchymal stem cell, MitoQ, reactive oxygen species, resveratrol | |||
|mipnetlab=CH Basel Kraehenbuehl S | |mipnetlab=CH Basel Kraehenbuehl S | ||
}} | }} | ||
{{Labeling | {{Labeling | ||
|area=Respiration, Pharmacology;toxicology | |||
|organism=Human | |||
|model cell lines=Other cell lines | |||
|preparations=Intact cells | |||
|couplingstates=ROUTINE, ETS | |||
|substratestates=CI, CI+II | |||
|instruments=Oxygraph-2k | |instruments=Oxygraph-2k | ||
}} | }} | ||
Revision as of 11:32, 29 January 2014
| Hirzel E, Lindinger PW, Maseneni S, Giese M, Rhein VV, Eckert A, Hoch M, KrĂ€henbĂŒhl S, Eberle AN (2013) Differential modulation of ROS signals and other mitochondrial parameters by the antioxidants MitoQ, resveratrol and curcumin in human adipocytes. J Recept Signal Transduct Res 33:304-312. |
Hirzel E, Lindinger PW, Maseneni S, Giese M, Rhein VV, Eckert A, Hoch M, KrĂ€henbĂŒhl S, Eberle AN (2013) J Recept Signal Transduct Res
Abstract: Mitochondrial reactive oxygen species (ROS) have been demonstrated to play an important role as signaling and regulating molecules in human adipocytes. In order to evaluate the differential modulating roles of antioxidants, we treated human adipocytes differentiated from human bone marrow-derived mesenchymal stem cells with MitoQ, resveratrol and curcumin. The effects on ROS, viability, mitochondrial respiration and intracellular ATP levels were examined. MitoQ lowered both oxidizing and reducing ROS. Resveratrol decreased reducing and curcumin oxidizing radicals only. All three substances slightly decreased state III respiration immediately after addition. After 24âh of treatment, MitoQ inhibited both basal and uncoupled oxygen consumption, whereas curcumin and resveratrol had no effect. Intracellular ATP levels were not altered. This demonstrates that MitoQ, resveratrol and curcumin exert potent modulating effects on ROS signaling in human adipocyte with marginal effects on metabolic parameters. âą Keywords: Adipocyte, antioxidant, curcumin, human mesenchymal stem cell, MitoQ, reactive oxygen species, resveratrol
âą O2k-Network Lab: CH Basel Kraehenbuehl S
Labels: MiParea: Respiration, Pharmacology;toxicology
Organism: Human
Preparation: Intact cells
Coupling state: ROUTINE, ETS"ETS" is not in the list (LEAK, ROUTINE, OXPHOS, ET) of allowed values for the "Coupling states" property.
HRR: Oxygraph-2k
