Difference between revisions of "Horan 2012 J Gerontol A Biol Sci Med Sci"
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{{Publication | {{Publication | ||
|title=Horan MP, Pichaud N, Ballard | |title=Horan MP, Pichaud N, Ballard JWO (2012) Review: Quantifying mitochondrial dysfunction in complex diseases of aging. J Gerontol A Biol Sci Med Sci 67:1022-35. https://doi.org/10.1093/gerona/glr263 | ||
|info=[http://www.ncbi.nlm.nih.gov/pubmed?term=Review%3A%20Quantifying%20Mitochondrial%20Dysfunction%20in%20Complex%20Diseases%20of%20Aging PMID: 22459622] | |info=[http://www.ncbi.nlm.nih.gov/pubmed?term=Review%3A%20Quantifying%20Mitochondrial%20Dysfunction%20in%20Complex%20Diseases%20of%20Aging PMID: 22459622 Open Access] | ||
|authors=Horan MP, Pichaud N, Ballard | |authors=Horan MP, Pichaud N, Ballard JWO | ||
|year=2012 | |year=2012 | ||
|journal=J Gerontol A Biol Sci Med Sci | |journal=J Gerontol A Biol Sci Med Sci | ||
|abstract=There is accumulating evidence that mitochondrial respiratory malfunction is associated with aging-associated complex diseases. However, progress in our understanding of these diseases has been hampered by the sensitivity and throughput of systems employed to quantify dysfunction and inherent limitations of the biological systems studied. In this review, we describe and contrast two methodologies that have been developed for measuring mitochondrial function to address the need for improved sensitivity and increased throughput. We then consider the utility of each methodology in studying three biological systems: isolated mitochondria, cultured cells, and cell fibers and tissues. Finally, we discuss the application of each methodology in the study of mitochondrial dysfunction in Alzheimer's disease, type 2 diabetes mellitus, and aging-associated autophagy impairment and mitochondrial malfunction. We conclude that the methodologies are complementary, and researchers may need to examine multiple biological systems to unravel complex diseases of aging. | |abstract=There is accumulating evidence that mitochondrial respiratory malfunction is associated with aging-associated complex diseases. However, progress in our understanding of these diseases has been hampered by the sensitivity and throughput of systems employed to quantify dysfunction and inherent limitations of the biological systems studied. In this review, we describe and contrast two methodologies that have been developed for measuring mitochondrial function to address the need for improved sensitivity and increased throughput. We then consider the utility of each methodology in studying three biological systems: isolated mitochondria, cultured cells, and cell fibers and tissues. Finally, we discuss the application of each methodology in the study of mitochondrial dysfunction in Alzheimer's disease, type 2 diabetes mellitus, and aging-associated autophagy impairment and mitochondrial malfunction. We conclude that the methodologies are complementary, and researchers may need to examine multiple biological systems to unravel complex diseases of aging. | ||
|keywords= | |keywords=Oroboros vs. Seahorse | ||
|mipnetlab=AU Sydney Ballard JW, | |mipnetlab=AU Sydney Ballard JW, | ||
}} | }} | ||
== Continue the discussion == | |||
* [[O2k-pH ISE-Module|O2k and measurement of acidification rate: '''O2k-pH ISE-Module''']] | |||
* [[Talk:Rogers 2011 PLoS One|On isolated mitochondria]] | |||
:::* '''Further information''' | |||
::::» [[O2k specifications]] | |||
::::» [[Comparison of respirometric methods]] | |||
== Cited by == | |||
{{Template:Cited by Krako Jakovljevic 2021 BEC PD}} | |||
{{Labeling | {{Labeling | ||
|area=Respiration, Instruments;methods, mt-Medicine | |area=Respiration, Instruments;methods, mt-Medicine | ||
|diseases=Aging; senescence, Alzheimer's, Diabetes, Neurodegenerative | |diseases=Aging;senescence, Alzheimer's, Diabetes, Neurodegenerative | ||
|instruments=Oxygraph-2k | |instruments=Oxygraph-2k | ||
|additional=Comparison of respirometric methods, MitoFit2022QC | |||
}} | }} | ||
Latest revision as of 10:25, 5 May 2022
| Horan MP, Pichaud N, Ballard JWO (2012) Review: Quantifying mitochondrial dysfunction in complex diseases of aging. J Gerontol A Biol Sci Med Sci 67:1022-35. https://doi.org/10.1093/gerona/glr263 |
Horan MP, Pichaud N, Ballard JWO (2012) J Gerontol A Biol Sci Med Sci
Abstract: There is accumulating evidence that mitochondrial respiratory malfunction is associated with aging-associated complex diseases. However, progress in our understanding of these diseases has been hampered by the sensitivity and throughput of systems employed to quantify dysfunction and inherent limitations of the biological systems studied. In this review, we describe and contrast two methodologies that have been developed for measuring mitochondrial function to address the need for improved sensitivity and increased throughput. We then consider the utility of each methodology in studying three biological systems: isolated mitochondria, cultured cells, and cell fibers and tissues. Finally, we discuss the application of each methodology in the study of mitochondrial dysfunction in Alzheimer's disease, type 2 diabetes mellitus, and aging-associated autophagy impairment and mitochondrial malfunction. We conclude that the methodologies are complementary, and researchers may need to examine multiple biological systems to unravel complex diseases of aging. • Keywords: Oroboros vs. Seahorse
• O2k-Network Lab: AU Sydney Ballard JW
Continue the discussion
- Further information
Cited by
- Krako Jakovljevic N, Ebanks B, Katyal G, Chakrabarti L, Markovic I, Moisoi N (2021) Mitochondrial homeostasis in cellular models of Parkinson’s Disease. Bioenerg Commun 2021.2. https://doi.org/10.26124/bec:2021-0002
Labels: MiParea: Respiration, Instruments;methods, mt-Medicine
Pathology: Aging;senescence, Alzheimer's, Diabetes, Neurodegenerative
HRR: Oxygraph-2k
Comparison of respirometric methods, MitoFit2022QC
