Inorganic phosphate: Difference between revisions
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{{MitoPedia | {{MitoPedia | ||
|abbr= | |abbr=P<sub>i</sub> | ||
|description= | |description='''Inorgnic phosphate''' (Pi) is a salt of phosphoric acid. In solution near physiological pH, the species HPO<sub>4</sub><sup>2-</sup> and H<sub>2</sub>PO<sub>4</sub><sup>-</sup> dominate. | ||
|info=[[ | ''See also'': [[Phosphate carrier]] (Pic). | ||
|info=[[Scheibye-Knudsen 2009 Eur J Appl Physiol]] | |||
}} | }} | ||
{{MitoPedia topics | {{MitoPedia topics | ||
|mitopedia topic=Substrate and metabolite | |mitopedia topic=Substrate and metabolite | ||
}} | }} | ||
== Application in HRFR == | |||
:::: The P<sub>i</sub> concentration in mitochondrial respiration media should be saturating for measurement of OXPHOS capacity (10 mM in [[MiR05-Kit]]) with [[HRFR]]. Many media used in mitochondrial respirometric studies contain inorganic phosphate at lower concentraitons (2 mM), which may not be saturating respiration in all pathway control states. It is not easy to isolate mitochondria in buffers without phosphate to study [[LEAK]] respiration limited by the absence of phosphate. |
Revision as of 08:22, 26 September 2018
Description
Inorgnic phosphate (Pi) is a salt of phosphoric acid. In solution near physiological pH, the species HPO42- and H2PO4- dominate. See also: Phosphate carrier (Pic).
Abbreviation: Pi
Reference: Scheibye-Knudsen 2009 Eur J Appl Physiol
MitoPedia topics:
Substrate and metabolite
Application in HRFR
- The Pi concentration in mitochondrial respiration media should be saturating for measurement of OXPHOS capacity (10 mM in MiR05-Kit) with HRFR. Many media used in mitochondrial respirometric studies contain inorganic phosphate at lower concentraitons (2 mM), which may not be saturating respiration in all pathway control states. It is not easy to isolate mitochondria in buffers without phosphate to study LEAK respiration limited by the absence of phosphate.