Jacobs 2012 FASEB J

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Jacobs R, Siebenmann C, Hug M, Toigo M, Meinild AK, Lundby C (2012) Twenty-eight days at 3454-m altitude diminishes respiratory capacity but enhances efficiency in human skeletal muscle mitochondria. FASEB J 90:5192-200.

» PMID: 22968913 Open Access

Jacobs R, Siebenmann C, Hug M, Toigo M, Meinild AK, Lundby C (2012) FASEB J

Abstract: Modifications of skeletal muscle mitochondria following exposure to high altitude (HA) are generally studied by morphological examinations and biochemical analysis of expression. The aim of this study was to examine tangible measures of mitochondrial function following a prolonged exposure to HA. For this purpose, skeletal muscle biopsies were obtained from 8 lowland natives at sea level (SL) prior to exposure and again after 28 d of exposure to HA at 3454 m. High-resolution respirometry was performed on the muscle samples comparing respiratory capacity and efficiency. Exercise capacity was assessed at SL and HA. Respirometric analysis revealed that mitochondrial respiratory capacity diminished in Complex I- and Complex II-specific respiration in addition to a loss of maximal State-3 oxidative phosphorylation capacity from SL to HA, all independent from alterations in mitochondrial content. LEAK control coupling, respiratory control ratio, and oligomycin-induced LEAK respiration, all measures of mitochondrial efficiency, improved in response to HA exposure. SL respiratory capacities correlated with measures of exercise capacity near SL, whereas mitochondrial efficiency correlated best with exercise capacity following HA. This data demonstrate that 1 mo of exposure to HA reduces respiratory capacity in human skeletal muscle; however, the efficiency of electron transport improves. Keywords: High altitude (HA), Exercise capacity

O2k-Network Lab: CH Zurich Lundby C, US CO Colorado Springs Jacobs RA

Labels: MiParea: Respiration, mt-Biogenesis;mt-density, Exercise physiology;nutrition;life style 

Stress:Ischemia-reperfusion  Organism: Human  Tissue;cell: Skeletal muscle  Preparation: Permeabilized tissue 

Regulation: ADP, Coupling efficiency;uncoupling, Uncoupler  Coupling state: LEAK, OXPHOS, ET  Pathway: N, S  HRR: Oxygraph-2k