Difference between revisions of "Jacobs 2013 J Gerontol A Biol Sci Med Sci"
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|preparations=Permeabilized tissue | |preparations=Permeabilized tissue | ||
|enzymes=Complex I, Complex III | |enzymes=Complex I, Complex III | ||
|diseases=Aging; senescence | |diseases=Aging;senescence | ||
|couplingstates=LEAK, OXPHOS, ETS | |couplingstates=LEAK, OXPHOS, ETS | ||
|substratestates=CI, CII, ETF, CI | |substratestates=CI, CII, ETF, CI&II, ROX | ||
|instruments=Oxygraph-2k | |instruments=Oxygraph-2k | ||
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Revision as of 15:49, 11 February 2015
Jacobs RA, Diaz V, Soldini L, Haider T, Thomassen M, Nordsborg NB, Gassmann M, Lundby C (2013) Fast-twitch glycolytic skeletal muscle is predisposed to age-induced impairments in mitochondrial function. J Gerontol A Biol Sci Med Sci 68: 1010-1022. |
Jacobs RA, Diaz V, Soldini L, Haider T, Thomassen M, Nordsborg NB, Gassmann M, Lundby C (2013) J Gerontol A Biol Sci Med Sci
Abstract: The etiology of mammalian senescence is suggested to involve the progressive impairment of mitochondrial function; however, direct observations of age-induced alterations in actual respiratory chain function are lacking. Accordingly, we assessed mitochondrial function via high-resolution respirometry and mitochondrial protein expression in soleus, quadricep, and lateral gastrocnemius skeletal muscles, which represent type 1 slow-twitch oxidative muscle (soleus) and type 2 fast-twitch glycolytic muscle (quadricep and gastrocnemius), respectively, in young (10-12 weeks) and mature (74-76 weeks) mice. Electron transport through mitochondrial complexes I and III increases with age in quadricep and gastrocnemius, which is not observed in soleus. Mitochondrial coupling efficiency during respiration through complex I also deteriorates with age in gastrocnemius and shows a tendency (p = .085) to worsen in quadricep. These data demonstrate actual alterations in electron transport function that occurs with age and are dependent on skeletal muscle type. β’ Keywords: Senescence, slow-twitch oxidative muscle, type 2 fast-twitch glycolytic muscle
β’ O2k-Network Lab: CH Zurich Lundby C
Labels: MiParea: Respiration
Pathology: Aging;senescence
Organism: Mouse Tissue;cell: Skeletal muscle Preparation: Permeabilized tissue Enzyme: Complex I, Complex III
Coupling state: LEAK, OXPHOS, ETS"ETS" is not in the list (LEAK, ROUTINE, OXPHOS, ET) of allowed values for the "Coupling states" property.
HRR: Oxygraph-2k