Difference between revisions of "Jarolim 2004 FEMS Yeast Res"
| Line 1: | Line 1: | ||
{{Publication | {{Publication | ||
|title=Jarolim S, Millen J, Heeren G, Laun P, Goldfarb DS, Breitenbach M (2004) A novel assay for replicative lifespan in Saccharomyces cerevisiae. FEMS Yeast Research 5: 169. | |title=Jarolim S, Millen J, Heeren G, Laun P, Goldfarb DS, Breitenbach M (2004) A novel assay for replicative lifespan in Saccharomyces cerevisiae. FEMS Yeast Research 5: 169. | ||
|authors=Jarolim S, Millen J, Heeren G, Laun P, Goldfarb DS, Breitenbach M | |authors=Jarolim S, Millen J, Heeren G, Laun P, Goldfarb DS, Breitenbach M | ||
|year=2004 | |year=2004 | ||
|journal=FEMS Yeast Research | |journal=FEMS Yeast Research | ||
|abstract=The replicative lifespan of ''Saccharomyces cerevisiae'' is determined by both genetic and environmental factors. Many of the same factors determine the lifespan of metazoan animals. The lack of fast and reliable lifespan assays has limited the pace of yeast aging research. In this study we describe a novel strategy for assaying replicative lifespan in yeast, and apply it in a screening of mutants that are resistant to pro-oxidants. The assay reproduces the lifespan-shortening effects of deleting SIR2 and of growth in the presence of paraquat, a pro-oxidant. The lifespan-increasing activity of resveratrol is also reproduced. Compared to current assays, this new strategy promises to significantly increase the possible number of replicative-lifespan determinations. | |abstract=The replicative lifespan of ''Saccharomyces cerevisiae'' is determined by both genetic and environmental factors. Many of the same factors determine the lifespan of metazoan animals. The lack of fast and reliable lifespan assays has limited the pace of yeast aging research. In this study we describe a novel strategy for assaying replicative lifespan in yeast, and apply it in a screening of mutants that are resistant to pro-oxidants. The assay reproduces the lifespan-shortening effects of deleting SIR2 and of growth in the presence of paraquat, a pro-oxidant. The lifespan-increasing activity of resveratrol is also reproduced. Compared to current assays, this new strategy promises to significantly increase the possible number of replicative-lifespan determinations. | ||
|keywords= ''Saccharomyces cerevisiae'', Yeast, Longevity, Aging, Replicative lifespan | |keywords=''Saccharomyces cerevisiae'', Yeast, Longevity, Aging, Replicative lifespan | ||
|info=[http://www.ncbi.nlm.nih.gov/pubmed/15489200 PMID: 15489200] | |info=[http://www.ncbi.nlm.nih.gov/pubmed/15489200 PMID: 15489200] | ||
}} | }} | ||
Revision as of 10:42, 20 October 2010
| Jarolim S, Millen J, Heeren G, Laun P, Goldfarb DS, Breitenbach M (2004) A novel assay for replicative lifespan in Saccharomyces cerevisiae. FEMS Yeast Research 5: 169. |
Jarolim S, Millen J, Heeren G, Laun P, Goldfarb DS, Breitenbach M (2004) FEMS Yeast Research
Abstract: The replicative lifespan of Saccharomyces cerevisiae is determined by both genetic and environmental factors. Many of the same factors determine the lifespan of metazoan animals. The lack of fast and reliable lifespan assays has limited the pace of yeast aging research. In this study we describe a novel strategy for assaying replicative lifespan in yeast, and apply it in a screening of mutants that are resistant to pro-oxidants. The assay reproduces the lifespan-shortening effects of deleting SIR2 and of growth in the presence of paraquat, a pro-oxidant. The lifespan-increasing activity of resveratrol is also reproduced. Compared to current assays, this new strategy promises to significantly increase the possible number of replicative-lifespan determinations. • Keywords: Saccharomyces cerevisiae, Yeast, Longevity, Aging, Replicative lifespan
Labels:
Organism: Yeast; Fungi"Yeast; Fungi" is not in the list (Human, Pig, Mouse, Rat, Guinea pig, Bovines, Horse, Dog, Rabbit, Cat, ...) of allowed values for the "Mammal and model" property.
Regulation: Respiration; OXPHOS; ETS Capacity"Respiration; OXPHOS; ETS Capacity" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property.
HRR: Oxygraph-2k
