Jasinska 2023 MiPschool Obergurgl

Jasinska 2023 MiPschool Obergurgl

Modulation of mitochondrial large conductance potassium channels activity by infrared light.

Link: MiPschool Obergurgl 2023

Jasinska Joanna (2023)

Event: MiPschool Obergurgl 2023

Authors: Jasinska Joanna, Bednarczyk Piotr, Kalenik B, Kulawiak Bogusz, Wrzosek A, Szewczyk Adam

Recent studies point out that mitochondria are not only a source of ATP in the cell, but more and more data indicate their role related to Ca2+ buffering, production of reactive oxygen species (ROS) and activation of intracellular signaling pathways of necrosis and apoptosis. Recent studies clearly indicate that mitochondrial potassium channels (mitoK) present in the inner mitochondrial membrane play an important protective role in the ischemia-reperfusion processes of myocardial cell damage. These results were obtained using low molecular weight chemicals. Due to the lack of selective modulators of potassium channels, we opted for an alternative approach to modulate the activity of mitoK channels by changing the redox state of the respiratory chain, which we have demonstrated in previous studies. Some respiratory chain proteins are thought to absorb infrared (IR) light. Cytochrome c oxidase (COX) may be important in these mechanisms because it has four metal redox centers: binuclear CuA, CuB, heme a, and heme a3. All these metal centers are able to absorb light waves in the IR region. Data obtained in our laboratory indicate that COX may be functionally linked to mitochondrial high-conductance Ca2+-activated potassium channels (mitoBKCa) in the U87 cell line1. Using the patch-clamp technique with the illumination system, we exposed the mitoBKCa channel. We observed that in the presence of ferricyanide, channel activity was inhibited and that mitoBK channel activity could be restored by 820 nm illumination, suggesting that COX is involved in the modulation of mitoBK channel activity.

1. Szewczyk A and Bednarczyk P (2018) Modulation of the Mitochondrial Potassium Channel Activity by Infrared Light. https://doi.org/10.1016/j.bpj.2017.11.288

Affiliations and acknowledgements

Jasińska Joanna¹, Bednarczyk P², Kalenik B¹, Kulawiak B¹, Wrzosek A¹, Szewczyk A¹

1. Lab. of Intracellular Ion Channels, Nencki Institute of Experimental Biology PAS, Poland
2. Dept of Physics and Biophysics, Institute of Biology, Warsaw Univ of Life Sciences -SGGW, Poland

Corresponding author: [email protected]

Funding: This study was supported by the Polish National Science Center (grants No.2019/34/A/NZ1/ 00352 to AS).

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Event: E2