Kaya 2013 Biochim Biophys Acta

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Kaya L, Meissner B, Riedl MC, Muik M, Schwarzer C, Ferraguti F, Sarg B, Lindner H, Schweigreiter R, Knaus HG, Romanin C, Bandtlow CE (2013) Direct association of the reticulon protein RTN1A with the ryanodine receptor 2 in neurons. Biochim Biophys Acta 1833:1421-33.

» PMID: 23454728 Open Access

Kaya L, Meissner B, Riedl MC, Muik M, Schwarzer C, Ferraguti F, Sarg B, Lindner H, Schweigreiter R, Knaus HG, Romanin C, Bandtlow CE (2013) Biochim Biophys Acta

Abstract: RTN1A is a reticulon protein with predominant localization in the endoplasmic reticulum (ER). It was previously shown that RTN1A is expressed in neurons of the mammalian central nervous system but functional information remains sparse. To elucidate the neuronal function of RTN1A, we chose to focus our investigation on identifying possible novel binding partners specifically interacting with the unique N-terminus of RTN1A. Using a nonbiased approach involving GST pull-downs and MS analysis, we identified the intracellular calcium release channel ryanodine receptor 2 (RyR2) as a direct binding partner of RTN1A. The RyR2 binding site was localized to a highly conserved 150-amino acid residue region. RTN1A displays high preference for RyR2 binding in vitro and in vivo and both proteins colocalize in hippocampal neurons and Purkinje cells. Moreover, we demonstrate the precise subcellular localization of RTN1A in Purkinje cells and show that RTN1A inhibits RyR channels in [3H]ryanodine binding studies on brain synaptosomes. In a functional assay, RTN1A significantly reduced RyR2-mediated Ca2+ oscillations. Thus, RTN1A and RyR2 might act as functional partners in the regulation of cytosolic Ca2+ dynamics in neurons. Keywords: Reticulon, Brain, Protein–protein interaction, Calcium homeostasis


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Organism: Mouse, Rat  Tissue;cell: Nervous system