Klingenberg 1970 Eur J Biochem

Klingenberg M (1970) Localization of the glycerol-phosphate dehydrogenase in the outer phase of the mitochondrial inner membrane. Eur J Biochem 13:247-52.

» PMID: 5439930

Klingenberg M (1970) Eur J Biochem

Abstract: Evidence for the localization of the mitochondrial glycerol-phosphate dehydrogenase in the outer phase of the inner membrane is presented.

1. Glycerol-phosphate does not permeate through the inner membrane to the matrix space in a number of mitochondria which actively oxidize glycerol-phosphate.
2. The impermeability of the inner membrane to ferricyanide is demonstrated and this forms a basis for elucidating membrane localization of carriers.
3. In mitochondria from various tissues ferricyanide can interact directly with glycerolphosphate dehydrogenase but not with succinate dehydrogenase.
4. The oxidation of glycerol-phosphate, in contrast to that of succinate, is insensitive to substrate depletion brought about by uncouplers.
5. The results are interpreted to show that both the substrate and the acceptor-site of glycerol-phosphate dehydrogenase are exposed to the outer side of the inner membrane. Thus, for the operation of the glycerol-phosphate dehydrogenase, mitochondria do not require translocases for glycerol-phosphate and dihydroxyacetone-phosphate.

Labels: MiParea: Respiration 

Preparation: Isolated mitochondria  Enzyme: Complex II;succinate dehydrogenase  Regulation: Uncoupler  Coupling state: ET  Pathway: Gp 

Glycerol-phosphate dehydrogenase, Uncoupler