Difference between revisions of "Kraft 2004 Thesis Universität Halle-Wittenberg - Germany"
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{{Publication | {{Publication | ||
|title=Kraft A (2004) Langzeitstabilität der Mitochondrienfunktion permeabilisierter Muskelfasern in der Diagnostik mitochondrialer Enzephalomyopathien. Thesis Universität Halle-Wittenberg - Germany 74pp. | |title=Kraft A (2004) Langzeitstabilität der Mitochondrienfunktion permeabilisierter Muskelfasern in der Diagnostik mitochondrialer Enzephalomyopathien. Thesis Universität Halle-Wittenberg - Germany 74pp. | ||
|info=[http://sundoc.bibliothek.uni-halle.de/diss-online/04/04H182/prom.pdf 04H182] | |info=[http://sundoc.bibliothek.uni-halle.de/diss-online/04/04H182/prom.pdf 04H182] | ||
|authors=Kraft A | |authors=Kraft A | ||
|year=2004 | |year=2004 | ||
|journal=Thesis Universität Halle-Wittenberg - Germany | |journal=Thesis Universität Halle-Wittenberg - Germany | ||
|abstract=We optimized the skinned fiber technique and high resolution respirometry developed for the examination of mitochondrial function. Storage of skeletal muscle fibers in ice-cooled storage buffer for 36 hours did not caused a significant change in mitochondrial function as determined by respiration of complex I and II dependent substrates. However, stable mitochondrial function was only guaranteed if cooling was without interruption. The newly established multiple substrate-inhibitor titration provides the possibility to measure complex I dependent functional deficits. This parameter was shown to be independent of the mitochondrial content of skeletal muscle fibers. Furthermore, we showed that shipment of samples in ice-cooled storage buffer by mail did not significantly influence mitochondrial function measured by respirometry. | |abstract=We optimized the skinned fiber technique and high resolution respirometry developed for the examination of mitochondrial function. Storage of skeletal muscle fibers in ice-cooled storage buffer for 36 hours did not caused a significant change in mitochondrial function as determined by respiration of complex I and II dependent substrates. However, stable mitochondrial function was only guaranteed if cooling was without interruption. The newly established multiple substrate-inhibitor titration provides the possibility to measure complex I dependent functional deficits. This parameter was shown to be independent of the mitochondrial content of skeletal muscle fibers. Furthermore, we showed that shipment of samples in ice-cooled storage buffer by mail did not significantly influence mitochondrial function measured by respirometry. | ||
|keywords=skinned fiber technique, respirometry, mitochondria, mitochondrial function, substrate-inhibitor-titration, mitochondrial encephalomyopathy, shipment of samples | |keywords=skinned fiber technique, respirometry, mitochondria, mitochondrial function, substrate-inhibitor-titration, mitochondrial encephalomyopathy, shipment of samples | ||
}} | }} | ||
{{Labeling | {{Labeling | ||
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|preparations=Permeabilized tissue | |preparations=Permeabilized tissue | ||
|couplingstates=LEAK, OXPHOS | |couplingstates=LEAK, OXPHOS | ||
|substratestates=CI, CII, CI | |substratestates=CI, CII, CI&II, ROX | ||
|instruments=Oxygraph-2k | |instruments=Oxygraph-2k | ||
}} | }} |
Revision as of 16:14, 11 February 2015
Kraft A (2004) Langzeitstabilität der Mitochondrienfunktion permeabilisierter Muskelfasern in der Diagnostik mitochondrialer Enzephalomyopathien. Thesis Universität Halle-Wittenberg - Germany 74pp. |
» 04H182
Kraft A (2004) Thesis Universität Halle-Wittenberg - Germany
Abstract: We optimized the skinned fiber technique and high resolution respirometry developed for the examination of mitochondrial function. Storage of skeletal muscle fibers in ice-cooled storage buffer for 36 hours did not caused a significant change in mitochondrial function as determined by respiration of complex I and II dependent substrates. However, stable mitochondrial function was only guaranteed if cooling was without interruption. The newly established multiple substrate-inhibitor titration provides the possibility to measure complex I dependent functional deficits. This parameter was shown to be independent of the mitochondrial content of skeletal muscle fibers. Furthermore, we showed that shipment of samples in ice-cooled storage buffer by mail did not significantly influence mitochondrial function measured by respirometry. • Keywords: skinned fiber technique, respirometry, mitochondria, mitochondrial function, substrate-inhibitor-titration, mitochondrial encephalomyopathy, shipment of samples
Labels: MiParea: Respiration, Instruments;methods, mt-Medicine
Organism: Human
Tissue;cell: Skeletal muscle
Preparation: Permeabilized tissue
Coupling state: LEAK, OXPHOS
HRR: Oxygraph-2k