Krako 2013 J Alzheimers Dis: Difference between revisions
Bader Helga (talk | contribs) No edit summary |
Beno Marija (talk | contribs) No edit summary Β |
||
| (3 intermediate revisions by 2 users not shown) | |||
| Line 5: | Line 5: | ||
|year=2013 | |year=2013 | ||
|journal=J Alzheimers Dis | |journal=J Alzheimers Dis | ||
|abstract=The 7WD4 and 7PA2 cell lines, widely used as cellular models for | |abstract=The 7WD4 and 7PA2 cell lines, widely used as cellular models for Alzheimer's disease (AD), have been used to investigate the effects of amyloid-Ξ² protein precursor overexpression and amyloid-Ξ² (AΞ²) oligomer accumulation on mitochondrial function. Under standard culture conditions, both cell lines, compared to Chinese hamster ovary (CHO) control cells, displayed an ~5% decrease of O2 respiration as sustained by endogenous substrates. Functional impairment of the respiratory chain was found distributed among the protein complexes, though more evident at the level of complex I and complex IV. Measurements of ATP showed that its synthesis by oxidative phosphorylation is decreased in 7WD4 and 7PA2 cells by ~25%, this loss being partly compensated by glycolysis (Warburg effect). Compensation proved to be more efficient in 7WD4 than in 7PA2 cells, the latter cell line displaying the highest reactive oxygen species production. The strongest deficit was observed in mitochondrial membrane potential that is almost 40% and 60% lower in 7WD4 and 7PA2 cells, respectively, in comparison to CHO controls. All functional parameters point to a severe bioenergetic impairment of the AD cells, with the extent of mitochondrial dysfunction being correlated to the accumulation of AΞ² peptides and oligomers. | ||
the effects of amyloid- | |keywords=Alzheimer's disease; Amyloid-Ξ² oligomers; Bioenergetics; Mitochondrial dysfunction | ||
function. Under standard culture conditions, both cell lines, compared to Chinese hamster ovary (CHO) control cells, displayed | |mipnetlab=IT Rome Sarti P | ||
an | |||
found distributed among the protein complexes, though more evident at the level of complex I and complex IV. Measurements | |||
of ATP showed that its synthesis by oxidative phosphorylation is decreased in 7WD4 and 7PA2 cells by | |||
partly compensated by glycolysis (Warburg effect). Compensation proved to be more efficient in 7WD4 than in 7PA2 cells, the | |||
latter cell line displaying the highest reactive oxygen species production. The strongest deficit was observed in mitochondrial | |||
membrane potential that is almost 40% and 60% lower in 7WD4 and 7PA2 cells, respectively, in comparison to CHO controls. | |||
All functional parameters point to a severe bioenergetic impairment of the AD cells, with the extent of mitochondrial dysfunction | |||
being correlated to the accumulation of | |||
|keywords= | |||
|mipnetlab=IT | |||
}} | }} | ||
{{Labeling | {{Labeling | ||
|area=Respiration, Comparative MiP;environmental MiP | |area=Respiration, Comparative MiP;environmental MiP | ||
| | |diseases=Alzheimer's | ||
|tissues=CHO | |||
|preparations=Permeabilized cells | |preparations=Permeabilized cells | ||
|enzymes=TCA cycle and matrix dehydrogenases | |enzymes=TCA cycle and matrix dehydrogenases | ||
|topics=mt-Membrane potential | |topics=mt-Membrane potential | ||
|couplingstates=LEAK, OXPHOS | |couplingstates=LEAK, OXPHOS | ||
| | |pathways=N, S, CIV | ||
|instruments=Oxygraph-2k | |instruments=Oxygraph-2k | ||
}} | }} | ||
Latest revision as of 11:20, 21 January 2020
| Krako N, Magnifico MC, Arese M, Meli G, Forte E, Lecci A, Manca A, GiuffrΓ¨ A, Mastronicola D, Sarti P, Cattaneo A (2013) Characterization of mitochondrial dysfunction in the 7PA2 cell model of Alzheimerβs disease. J Alzheimers Dis 37:747-58. |
Krako N, Magnifico MC, Arese M, Meli G, Forte E, Lecci A, Manca A, Giuffre A, Mastronicola D, Sarti P, Cattaneo A (2013) J Alzheimers Dis
Abstract: The 7WD4 and 7PA2 cell lines, widely used as cellular models for Alzheimer's disease (AD), have been used to investigate the effects of amyloid-Ξ² protein precursor overexpression and amyloid-Ξ² (AΞ²) oligomer accumulation on mitochondrial function. Under standard culture conditions, both cell lines, compared to Chinese hamster ovary (CHO) control cells, displayed an ~5% decrease of O2 respiration as sustained by endogenous substrates. Functional impairment of the respiratory chain was found distributed among the protein complexes, though more evident at the level of complex I and complex IV. Measurements of ATP showed that its synthesis by oxidative phosphorylation is decreased in 7WD4 and 7PA2 cells by ~25%, this loss being partly compensated by glycolysis (Warburg effect). Compensation proved to be more efficient in 7WD4 than in 7PA2 cells, the latter cell line displaying the highest reactive oxygen species production. The strongest deficit was observed in mitochondrial membrane potential that is almost 40% and 60% lower in 7WD4 and 7PA2 cells, respectively, in comparison to CHO controls. All functional parameters point to a severe bioenergetic impairment of the AD cells, with the extent of mitochondrial dysfunction being correlated to the accumulation of AΞ² peptides and oligomers. β’ Keywords: Alzheimer's disease; Amyloid-Ξ² oligomers; Bioenergetics; Mitochondrial dysfunction
β’ O2k-Network Lab: IT Rome Sarti P
Labels: MiParea: Respiration, Comparative MiP;environmental MiP
Pathology: Alzheimer's
Tissue;cell: CHO
Preparation: Permeabilized cells
Enzyme: TCA cycle and matrix dehydrogenases
Regulation: mt-Membrane potential
Coupling state: LEAK, OXPHOS
Pathway: N, S, CIV
HRR: Oxygraph-2k
